LOCUS       sequence-254286-       11626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11626)
  AUTHORS   Dukkipati A, Park HH, Waghray D, Fischer S, Garcia KC
  TITLE     BacMam system for high-level expression of recombinant soluble and 
            membrane glycoproteins for structural studies.
  JOURNAL   Protein Expr Purif. 2008 Dec;62(2):160-70. Epub 2008 Aug 22.
  PUBMED    18782620
REFERENCE   2  (bases 1 to 11626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-12"; volume: "62(2)"; pages: "160-70. Epub
            2008 Aug 22"
COMMENT     Sequence Label: pVLAD6
FEATURES             Location/Qualifiers
     source          1..11626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2208..2225
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_recomb     2659..3364
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             2659..3247
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     3'UTR           2904..3276
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     enhancer        3987..4366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4367..4570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4521..4541
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4567..4591
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4706..4838
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4821..4840
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4883..4901
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4955..4978
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4988..5023
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5024..5047
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     misc_feature    5051..5639
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5104..5124)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5522..5533)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5651..5772
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5662..5681)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5716..5735
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(5906..5997)
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(5964..5981)
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     misc_recomb     6001..6828
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             6061..6666
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     CDS             complement(6704..6828)
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     primer_bind     complement(10001..10018)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10001..10017)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10010..10032)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10226..10245)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10345..10367
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10405..10423)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10491..10595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10596..10664,10665..11456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10814..10833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gaattggccg ccggttagca
     1021 acttgtatgt taacacgccg acggcgtacc agtcaaacga acgtgcatag ttgtggtgtc
     1081 gaattttttc cggactaaaa tactccaacg tgccgtcgtg cacgctaagt gagttttcgt
     1141 gtttgcacaa tccgtaatcg caaacataca cgcgatcaag tgcttcgaaa tataagacat
     1201 tttcgagttt tatgtcgttg tgtatgaaat tgtgcttgtg caaatcgttg agcgcttcac
     1261 acagctgtct aataatattg ctaacaagtt ggtacgaaag ctcgccttta atttgtagtg
     1321 tttcaaataa atcgggacag tcaatgtagt ccatcacgat cacttggttg ttgatggaac
     1381 cgtgattaaa atagattttt ataaaatttg ggtggtcgct cattaactgg tgaactttta
     1441 tttcgtcagc gttaaaattg tgcgccgaaa ttgttttctg caaatacaat ttgctagtgg
     1501 gcttgtgcga taaaatagat attttgccaa agcgcccgtt gattatttta taccgagttt
     1561 tgacgttttt gcagttttcg taaaattgta ccagcgtttg cagcgtagca tttgtggtgg
     1621 ccatgacgaa tcgtagatat gaatctgtac aatcttattt attcaataat aggaacaaca
     1681 aaattgatgc gcatcaattt tttgaacgtg tcgatactgc ggaagcgcaa attatcaaaa
     1741 acaacattta cgacaacaca gtggtgctga acagagatgt tcttttaaat attttaaagc
     1801 ttgccaacga cgtttttgac aacaaagcat acatgtacgt cgacgacagc gaagtgtctc
     1861 gccactacaa tgctgtagta aaaatgaaaa ggcttgttat cggcgtgcga gcttgagaca
     1921 atctctttac aatacaattg cttacattga gcggttgtta aatatcggca cggtaaacga
     1981 tagcgaaatt acaatgctta tagcagattt ttacgatttg tattccaatt ataatattga
     2041 attgccacca ccccaagcgc tgcctcgaag cagacgacct tccgttgtgc agccagcggc
     2101 gcctgcgccg gtgcccacaa tcgtgcgcga acaaactaaa ccagaacaaa ttataccggc
     2161 ggcaccgccg ccaccacctt ctcccgtgcc taacattcca gcgcctccac caccaccacc
     2221 accatcgatg tctgaattgc cgcccgctcc accaatgccg acggaacctc aacccgctgc
     2281 acctttagac gacagacaac aattgttgga agctattaga aacgaaaaaa atcgcactcg
     2341 tctcagaccg gtcaaaccaa aaacggcgcc cgaaaccagt acaatagttg aggtgccgac
     2401 tgtgttgcct aaagagacat ttgagcctaa accgccgtct gcatcaccgc caccacctcc
     2461 gcctccgcct ccgccgccag ccccgcctgc gcctccaccg atggtagatt tatcatcagc
     2521 tccaccaccg ccgccattag tagatttgcc gtctgaaatg ttaccaccgc ctgcaccatc
     2581 gctttctaac gtgttgtctg aattaaaatc gggcacagtt agattgaaac ccgcccaaaa
     2641 acgcccgcaa tcagaaataa ttccaaaaag ctcaactaca aatttgatcg cggacgtgtt
     2701 agccgacaca attaataggc gtcgtgtggc tatggcaaaa tcgtcttcgg aagcaacttc
     2761 taacgacgag ggttgggacg acgacgataa tcggcctaat aaagctaaca cgcccgatgt
     2821 taaatatgtc caagctttat ttaacgtgtt tacgtcgagt caattgtaca ctaacgacag
     2881 tgatgaaaga aatacaaaag cgcataatat tttgaacgac gtcgaacctt tattacaaaa
     2941 caaaacacaa acgaatatcg acaaagctag attgctgcta caagatttgg caagttttgt
     3001 ggcgttgagc gaaaatccat tagatagtcc agccatcggt tcggaaaaac aacccttgtt
     3061 tgaaactaat cgaaacctat tttacaaatc tattgaggat ttaatattta aattcagata
     3121 taaagacgct gaaaatcatt tgattttcgc tctaacatac caccctaaag attataaatt
     3181 taatgaatta ttaaaatacg tacaacaatt gtctgtaaat caacaacgca cagaatctag
     3241 cgcttaataa atgtactaat aacaatgtat cgtgttttaa tacgccggac cagtgaacag
     3301 aggtgcgtct ggtgcaaact cctttacttt gaacaccagg gaaacttcaa ggagaatttc
     3361 ctcctcttca gcagagtcgg taccgatgta aacgatgggc ttgtagaagt tctcccagat
     3421 gacacgatcg atgaactgtt cgaacgagtt ggtgtactca gagtgaaggt tcattattgg
     3481 gcagccgccg cccttcttag ccaggctgat gcggtactcg ttgttgctgc ccacccatga
     3541 aggctcgacg atcctaatca cgtcatgagg tacatagtcg gggtcgcaac gcagagcgtg
     3601 ttgggccagg aatttgtaac aacggttggg tctagtggga cgcatgttga caacaaggaa
     3661 aacatccatc acttcttggt cgttaacaat ggggaagctg tcttccatga agcgggtcca
     3721 agtttccctg tagaactctt ttcctttcca tccaacgaca agcttcatcg tgtcgggttt
     3781 aacattacgg atttccttga agagagtgag tttttggttc ttgccgggtc ccaggaaagg
     3841 atcagatctt caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     3901 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     3961 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     4021 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     4081 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     4141 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4201 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga
     4261 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt
     4321 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     4381 gcagtacacc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     4441 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     4501 taataacccc gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     4561 aagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca
     4621 cagttaaatt gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag
     4681 aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga
     4741 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     4801 tggtcttact gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta
     4861 cagctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg agaattcacg
     4921 cgtggtacct ctagagtcga cccgggcggc cgcattggaa gttctgtttc aagggcctgg
     4981 ggccgcagaa gatcaagttg atccaagatt gattgatggc aagcatcacc atcaccatca
     5041 ccatcactag aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5101 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5161 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5221 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5281 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5341 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5401 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     5461 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5521 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5581 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5641 tgagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     5701 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     5761 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     5821 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaggatccg cgcccgatgg
     5881 tgggacggta tgaataatcc ggaatattta taggtttttt tattacaaaa ctgttacgaa
     5941 aacagtaaaa tacttattta tttgcgagat ggttatcatt ttaattatct ccatgatatc
     6001 agcaactata tattgataga catttccagt ttgtgatatt agtttgtgcg tctcattaca
     6061 atggctgtta tttttaacaa caaacaactg ctcgcagaca atagtataga aaagggaggt
     6121 gaactgtttt tgtttaacgg ttcgtacaac attttggaaa gttatgttaa tccggtgctg
     6181 ctaaaaaatg gtgtaattga actagaagaa gctgcgtact atgccggcaa catattgtac
     6241 aaaaccgacg atcccaaatt cattgattat ataaatttaa taattaaagc aacacactcc
     6301 gaagaactac cagaaaatag cactgttgta aattacagaa aaactatgcg cagcggtact
     6361 atacacccca ttaaaaaaga catatatatt tatgacaaca aaaaatttac tctatacgat
     6421 agatacatat atggatacga taataactat gttaattttt atgaggagaa aaatgaaaaa
     6481 gagaaggaat acgaagaaga agacgacaag gcgtctagtt tatgtgaaaa taaaattata
     6541 ttgtcgcaaa ttaactgtga atcatttgaa aatgatttta aatattacct cagcgattat
     6601 aactacgcgt tttcaattat agataatact acaaatgttc ttgttgcgtt tggtttgtat
     6661 cgttaataaa aaacaaattt gacatttata attgttttat tattcaataa ttacaaatag
     6721 gattgagacc cttgcagttg ccagcaaacg gacagagctt gtcgacagtt ttcatttttt
     6781 ggcagtttgg tggtttatac acatcttggg atttgattaa aagatgcatg acttcaccca
     6841 cacacttgga gtcgtggttg tagaaatgcg tcagagcctt gattaaacat cggttttcac
     6901 acttggcaca cgaaaccacg tttgcaatgt aacagtttag tataaacctc ttcctgtaca
     6961 taccgccgcg cggtacattg ttttctttca aatcgttcaa tatttttttt atacacggag
     7021 gcatattaat cttgttgttc agcatgtcga cgacattttt ctttccttct acgcattctt
     7081 tcatacaaat gtttctcttg ctgcgttttt taaaattgga atttttaatt tcattaatag
     7141 tgggcggagc cgttaacagc atgtacaatc gcagtccgga aattttgacc aaaaccccgc
     7201 cattgtagaa taccgtgtat ggagttaggg tcaatttatc aataaaatca tcgggatcaa
     7261 ttaaatatgt agctttcttg tctaaacatg acgctctaat gagcggactc cacacgttat
     7321 acgatgcatt cgccatactt cgcggcttct cgcaccagcg gtcctgattc gactaacggt
     7381 cgattggatg gcttaatagt tttaacgtca ccgacatact cggtaatttt gtagtcggca
     7441 gttcttttgg gcgtgatcgt tgtcactacg ggggtcatgt catcgcttcg taaaaactta
     7501 ttacaatcaa agctcgtgcc ggaacgcgtg cacagatcgt gcacgttgcg tcgcaagcta
     7561 tttaattcgc gctttatttg atctctatca tagtcgtgtt gagatgcgac aacgggcgca
     7621 ttgttaattc ttgacgtgcg atacataata tattattgga taaagttgca ttaaatgaaa
     7681 ctaacttaca agatggctag tttgttaaaa tacgcgctgc gcttgactcg ggaatacaaa
     7741 gaaaacatta ttccacactt tgatcacttg actcgattgc gcgatttaat cgacggcatg
     7801 attaaaagcg aggatgtaca acgttttaat cgcactaatc gcaatgattt aatttcggct
     7861 tgcatgcaaa tcaacgttcg gacgtacatg cccaacgcca cgatagatat gcgcaaacaa
     7921 cccaactgta tatattttcg aatttgccaa tattgccact tggaggccga cgtgccttcg
     7981 cccgacgatc attcggtgta cagatacttg tgcgtcgcgt gcggcacgcc gctggtcatc
     8041 gaccacccgc tcgacgtgtt cggccacacg gaggaaggcg tcaacgaact gctcgaggtg
     8101 cagcgagtca acgcgggcgg ggagttgtag gcgtcataac tatttattaa ataagataat
     8161 ttaaaaaatc gccgttaata tgcaaatcaa aactgtacta ttggcttttg cgatgtttgc
     8221 cgcgttgaac gcgcaacacg ttttagccgc ttgcgccgag accggcgccg tctgcgttca
     8281 taacgacgag tgttgtagcg gcgcatgttc ccccatattt aattattgct taccacaata
     8341 agtttagttt gcgttaaaaa acaatagttg ttgcaaaatc gacacacgtg tatcaaaatg
     8401 gctcgcgtta aaattggaga atttaaattt ggagaagata cgttcaacct gcggtacgtg
     8461 ctagagcgcg accaacaggt ccggttcgtg gccaaggacg tcgctaacag tttaaaatat
     8521 acagtttgtg ataaagctat acgtgttcat gtagacaata aatacaaatc gttatttgag
     8581 cagaccatcc aaaatggggg ccctacctct aacagcgtcg tgaaaagggg cgacccgctg
     8641 tatttgcagc cgcatacagt gctcattact aaatccggcg tgattcagct gattatgaag
     8701 tccaaattgc cttacgccat agaattacaa gaatggcttt tagaagaagt aattcctcaa
     8761 gtgctctgca ccggcaagta cgatcccgcg atcaaacaac gggaggagga gagcaaacag
     8821 ttggtgacta agctgattgc gacattcacc gagcacacaa acgcgctgca agcggtggtg
     8881 gcgcaaaaaa ccgaggaact tgttaaaaaa caagagttta ttgaacgcat cgttgccatc
     8941 aaggacaagc agatcgaggc caaagatttg caggtcacgc gcgtcatgac tgatctaaac
     9001 cgcatgtaca ccggcttcca ggaaaccatg caaaagaaag acgaaataat gcagaaaaaa
     9061 gacgcgcagg tcaccgattt agttgccaaa gtggtggatt tgtcggatcg cgcggttcaa
     9121 taccccgcgg acaagcgcaa acatccggtg ttgtgcgtga cgcgcgacgg cactacgttt
     9181 acggctatta ccggccaaaa gacgtacgtg gaaaaccaaa agcataaacg taacatcaac
     9241 gttgccaaca ttgtggtgga gaatatccgg cctaatccta ccgtcgattg gaacaacgcc
     9301 actgatcggc tacaagctaa acggagcaag cgaagcatag ttttggttcg ctggaagaag
     9361 cgcaacaatt tgaaaatagg ataaagtatt tgttaaagaa tgcaaataat attaattaaa
     9421 ttaataaatc ttgaacgtaa ttttgtcttt caattttgtg acctctggct ttttcgaatc
     9481 tatctatggc ttcctgcggc gcaataccca gggtgtgcat taaatatctg cacaccatgt
     9541 aaccggtgcg attaataccg tgtgtgcagt gcacgcccac caacatgccg ggacactttt
     9601 ctgtaaattc ttttaccgtg tcaataaatt cttgaactat gctttcaggc ggcaaagtct
     9661 ggccaggtac ttgaattttt ttgtataaca ggcccgcccg caaaaaatgc acaccatcat
     9721 aatatttaga cgtgttggtt aaatcgatta ttgctccaat actagggttt tgttttacta
     9781 tctgttctgc ggtccacata tcttcttcgc tagtcacgta cgcaaacaac tcgggccgca
     9841 aaggtgtttt aaaacatatc agattagaat cttttataac ttggccgcat tgtaaatagt
     9901 tgtgccaacg cgcgggaaac attttacacg tgctttcaat cttatctcat aaacgcaact
     9961 aaatatgatc cttcaactcg ctttacgagc caagcttggc actggccgtc gttttacaac
    10021 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    10081 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10141 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
    10201 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
    10261 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
    10321 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
    10381 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
    10441 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10501 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10561 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10621 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10681 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10741 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10801 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
    10861 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10921 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10981 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    11041 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    11101 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    11161 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    11221 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    11281 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    11341 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    11401 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    11461 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    11521 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    11581 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
