LOCUS       sequence-254299-       13339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mouse EPHB2 and GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Cortina C, Palomo-Ponce S, Iglesias M, Fernandez-Masip JL, Vivancos 
            A, Whissell G, Huma M, Peiro N, Gallego L, Jonkheer S, Davy A, 
            Lloreta J, Sancho E, Batlle E
  TITLE     EphB-ephrin-B interactions suppress colorectal cancer progression by
            compartmentalizing tumor cells.
  JOURNAL   Nat Genet. 2007 Nov;39(11):1376-83. Epub 2007 Sep 30.
  PUBMED    17906625
REFERENCE   2  (bases 1 to 13339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Genet."; date: "2007-11"; volume: "39(11)"; pages: "1376-83. Epub 
            2007 Sep 30"
COMMENT     Sequence Label: FUW-ubiquitin-EphB2-SV40-GFP (B2G)
FEATURES             Location/Qualifiers
     source          1..13339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2379..3590
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3427..3446
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     enhancer        6647..6838
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     promoter        6688..6985
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        6786..6996
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6855..6990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      7007..7016
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7013..7015,7016..7018,7019..7732)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7013..7015,7016..7018,7019..7729)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7058..7079)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7319..7338)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7666..7687
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7794..8382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7847..7867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8265..8276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8385..8401)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8386..8402)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8907..9087
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9113..9130)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9119..9343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9389..9817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9476..9495)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9686..9707
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9831..10160
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10011..10146
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        10208..10255
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10274..10648
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10778..10899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10815..10834)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10869..10888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10948..10964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10948..10964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10961..10983)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10972..10988
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10996..11002,11003..11020,11021..11026))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11041..11062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11179..11196)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11350..11938)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11430..11449)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12109..12900,12901..12969))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12732..12751
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12970..13074)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13149..13168)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaaggccgg cctgcagcgg cctccgcgcc gggttttggc
     2401 gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc acgtcagacg aagggcgcag
     2461 cgagcgtcct gatccttccg cccggacgct caggacagcg gcccgctgct cataagactc
     2521 ggccttagaa ccccagtatc agcagaagga cattttagga cgggacttgg gtgactctag
     2581 ggcactggtt ttctttccag agagcggaac aggcgaggaa aagtagtccc ttctcggcga
     2641 ttctgcggag ggatctccgt ggggcggtga acgccgatga ttatataagg acgcgccggg
     2701 tgtggcacag ctagttccgt cgcagccggg atttgggtcg cggttcttgt ttgtggatcg
     2761 ctgtgatcgt cacttggtga gtagcgggct gctgggctgg ccggggcttt cgtggccgcc
     2821 gggccgctcg gtgggacgga agcgtgtgga gagaccgcca agggctgtag tctgggtccg
     2881 cgagcaaggt tgccctgaac tgggggttgg ggggagcgca gcaaaatggc ggctgttccc
     2941 gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg tcgttgaaac aaggtggggg
     3001 gcatggtggg cggcaagaac ccaaggtctt gaggccttcg ctaatgcggg aaagctctta
     3061 ttcgggtgag atgggctggg gcaccatctg gggaccctga cgtgaagttt gtcactgact
     3121 ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta tggcggtgcc gttgggcagt
     3181 gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt gacgtcaccc gttctgttgg
     3241 cttataatgc agggtggggc cacctgccgg taggtgtgcg gtaggctttt ctccgtcgca
     3301 ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg acaggcgccg gacctctggt
     3361 gaggggaggg ataagtgagg cgtcagtttc tttggtcggt tttatgtacc tatcttctta
     3421 agtagctgaa gctccggttt tgaactatgc gctcggggtt ggcgagtgtg ttttgtgaag
     3481 ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt cagtgttaga
     3541 ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac aggatccacc
     3601 atgggagccc gggtccccgt tctgcccggg ctggatggct cattctgctg gctgctgctg
     3661 ctgccgctgc tagccgccgt ggaagaaacc ctgatggact ctacgacagc aacggctgag
     3721 ctgggctgga tggtacatcc cccatcaggg tgggaagagg tgagcggcta cgacgagaac
     3781 atgaacacta tccgtaccta ccaggtgtgc aatgtctttg agtcaagcca gaacaactgg
     3841 ctgcggacca aattcatccg gcgccgtggc gcccaccgta tccacgtgga gatgaagttc
     3901 tcggtgcgtg actgcagcag cattcccagc gtgccgggct cctgcaagga gaccttcaac
     3961 ctctactact atgaggctga ttttgactta gccaccaaaa cctttcccaa ctggatggag
     4021 aatccgtggg tgaaggtgga caccatcgcg gccgatgaga gcttctctca ggtggacctg
     4081 ggtggccgcg tcatgaaaat caacactgag gtgcgaagct tcggtcctgt gtcccgcaat
     4141 ggtttctacc tggccttcca ggactacggc ggctgtatgt ccctcattgc tgtgcgcgtc
     4201 ttctaccgga agtgcccccg aatcatccag aatggtgcca tcttccagga gacactatcg
     4261 ggggctgaga gcacttcgct ggtggcagct cggggcagct gcatcgccaa tgctgaagaa
     4321 gtggacgtgc ccatcaaact ctactgtaac ggggacggcg aatggctggt gcccatcggt
     4381 cgctgcatgt gcaaggcggg cttcgaggct gtggagaacg gcaccgtctg ccgaggttgt
     4441 ccatcaggaa ccttcaaggc caaccaaggg gacgaagcct gcacccactg tcccatcaac
     4501 agccgcacca cctctgaggg tgccaccaac tgtgtatgcc gcaacggcta ctacagggcc
     4561 gacctggacc ccttagacat gccttgcaca accatcccct ctgcgcccca ggctgtgatc
     4621 tccagcgtca acgagacatc cctcatgcta gagtggaccc caccccgaga ctccgggggt
     4681 cgcgaggatc ttgtttacaa catcatctgc aagagctgtg gctccggccg gggcgcatgc
     4741 acgcgctgcg gggacaacgt gcagtacgcg ccccgccagc tgggcctgac tgagccgcgc
     4801 atctacatca gtgacctgct ggcacacacg cagtacacct tcgagatcca ggccgtgaat
     4861 ggtgtgaccg accagagtcc cttctcacct cagttcgcct ctgtgaacat caccaccaac
     4921 caagcagcac catcggccgt gtccatcatg caccaggtga gccgcactgt ggacagcatc
     4981 accctgtcgt ggtcccagcc agaccagccc aacggtgtga tcctggacta cgagctgcag
     5041 tactatgaga aggagctcag tgagtacaac gccacggcca taaaaagccc caccaacaca
     5101 gtcactgtgc agggcctcaa agccggcgcc atctatgtct tccaggtgcg ggcacgcacc
     5161 gttgcaggct atgggcgcta cagtggcaag atgtacttcc aaaccatgac agaagccgag
     5221 taccagacca gcatcaagga aaagctaccc ctcatcgttg gctcctccgc cgccggctta
     5281 gtcttcctca tcgctgtggt cgtcattgcc atcgtatgta acagacgggg gtttgagcgt
     5341 gccgactcag agtacacgga caagctacag cactacacca gcggacacat gaccccaggc
     5401 atgaagatct atatagatcc tttcacctat gaagatccta atgaggcagt gcgggagttt
     5461 gccaaggaaa ttgacatctc ctgtgtcaag attgagcagg tgattggagc aggggaattt
     5521 ggtgaggtct gcagtggcca tttgaagctg ccaggcaaga gagagatctt tgtagccatc
     5581 aagaccctca agtcaggata cacggagaaa cagcgccggg acttcctgag tgaggcatcc
     5641 atcatgggcc agttcgacca ccccaatgtc atccatctgg aaggggttgt caccaagagc
     5701 acacctgtca tgatcatcac tgaattcatg gagaatggat ctctggactc cttcctccgg
     5761 caaaatgatg ggcagttcac agtcatccaa ctggtgggca tgctgagggg cattgcagcc
     5821 ggcatgaagt acctggcgga catgaactac gtgcaccgtg accttgctgc tcgaaacatc
     5881 ctcgtcaaca gtaacctggt gtgtaaggtg tctgactttg ggctctcacg cttcctggag
     5941 gatgacacgt ctgaccccac ctataccagc gctctgggtg ggaagatccc catccgttgg
     6001 acggcaccgg aagccatcca gtaccggaaa ttcacctcgg ccagtgatgt gtggagctat
     6061 ggcatcgtca tgtgggaggt gatgtcctac ggggaacgac cctactggga catgaccaat
     6121 caagacgtaa tcaacgccat tgaacaggac tacagactac ctccgcccat ggactgccct
     6181 agcgccctgc accagctcat gctggactgc tggcagaagg accgcaacca ccggcccaag
     6241 ttcggccaga ttgtcaacac gctggacaag atgatccgaa accccaacag cctcaaagcc
     6301 atggcacccc tgtcctctgg catcaacctg ccactgctgg accgcacgat accggactac
     6361 accagcttta acacagtgga tgagtggcta gaggccatca agatgggcca gtacaaggag
     6421 agctttgcca acgccggctt cacctctttc gacgttgtat ctcagatgat gatggaggac
     6481 attctccgcg ttggggtcac tctagctggc caccagaaaa aaatcctgaa cagtatccag
     6541 gtgatgcggg cccagatgaa ccagatccag tctgtagagg tttgaaccgg tgcgcagcac
     6601 catggcctga aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga
     6661 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6721 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     6781 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     6841 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6961 agaagtagtg aggaggcttt tttggaggcc taggcttttg cagctagcca ccatggtgag
     7021 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     7081 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     7141 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     7201 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     7261 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7321 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7381 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7441 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7501 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     7561 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     7621 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7681 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aactcgagca
     7741 tgcatctaga gggccctttt tggcgcgccg aattcgatat caagcttatc gataatcaac
     7801 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7861 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7921 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7981 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     8041 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     8101 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     8161 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     8221 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     8281 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     8341 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     8401 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     8461 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     8521 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     8581 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     8641 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     8701 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     8761 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     8821 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     8881 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8941 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9001 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9061 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     9121 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     9181 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     9241 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     9301 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     9361 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     9421 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9481 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9541 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9601 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9661 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9721 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9781 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     9841 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9901 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9961 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10021 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10081 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10141 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    10201 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
    10261 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
    10321 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
    10381 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
    10441 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
    10501 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
    10561 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
    10621 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
    10681 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10741 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    10801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    10921 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    10981 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    11041 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    11101 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    11161 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    11221 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    11281 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    11341 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    11401 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    11461 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    11521 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    11581 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    11641 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    11701 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11761 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    11821 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11881 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11941 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12001 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    12061 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    12121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    12181 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    12241 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    12301 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    12361 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    12421 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    12481 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    12541 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    12601 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    12661 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12721 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12781 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12841 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12901 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12961 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    13021 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    13081 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    13141 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    13201 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    13261 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
    13321 cgggccagat atacgcgtt
//
