LOCUS       sequence-25430-e         634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: MSCV FLIPi P2G_Thy1.1 PTEN
FEATURES             Location/Qualifiers
     source          1..634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(20..53)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     ncRNA           complement(126..252)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(280..294)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(322..443)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 tacaagtagt ctagaagcca taacttcgta tagtacacat tatacgaagt tatgtttatg
       61 catacctgga ggaaaaaaaa aaggggagag tcaggggttt aaacgcatta gtcttccaat
      121 tgaaaaaagt gatttaattt ataccatttt aattcagctt tgtaaaaatg tatcaaagag
      181 atagcaaggt attcagtttt agtaaacaag ataattgctc ctaaagtagc cccttgaatt
      241 ccgaggcagt aggcaaggtg aaactatact ttacaaatac atctgtggct tcactatttg
      301 taaagtatag tttcaccgcg ctcactgtca acagcaatat accttctcga gccttctgtt
      361 gggttaacct gaagaagtaa tcccagcaag tgtttccaag atgtgcaggc aacgattctg
      421 taaagtactg aagcctcatt caacatagta tatgtgctgc cgaagcgagc acttaacaag
      481 gcttgcggcc atttaaatgc tgctgctgcg gccgcccact cacctgcaat tgtcacagag
      541 aaatgaagtc cagggcttgg aggagggaga gggaaagcag cagcagcagc atccaggatg
      601 tgttctgaac caccaggctt atgccgccac actt
//
