LOCUS       sequence-25431-e         666 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: MSCV P2Gm FF
FEATURES             Location/Qualifiers
     source          1..666
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           77..198
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           225..239
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           267..393
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(503..523)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tctcggcatg gacgagctgt acaagtagcg gccgcaagcc ttgttaagtg ctcgcttcgg
       61 cagcacatat actatgttga atgaggcttc agtactttac agaatcgttg cctgcacatc
      121 ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct cgagaaggta
      181 tattgctgtt gacagtgagc gagctcccgt gaattggaat cctagtgaag ccacagatgt
      241 aggattccaa ttcagcggga gcctgcctac tgcctcggaa ttcaaggggc tactttagga
      301 gcaattatct tgtttactaa aactgaatac cttgctatct ctttgataca tttttacaaa
      361 gctgaattaa aatggtataa attaaatcac ttttttcaat tggaagacta atgcgtttaa
      421 actgtcgacc tgcagccaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
      481 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
      541 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
      601 cctggttgct gtctctttat gaggagttgg ggcccgttgt caggcaacgt ggcgtggtgt
      661 gcactg
//
