LOCUS       sequence-25432-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: MSCV P2Gm p53
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           62..183
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           211..225
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           253..379
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(489..509)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctgtacaagt agcggccgca agccttgtta agtgctcgct tcggcagcca catatactat
       61 gttgaatgag gcttcagtac tttacagaat cgttgcctgc acatcttgga aacacttgct
      121 gggattactt cttcaggtta acccaacaga aggctcgaga aggtatattg ctgttgacag
      181 tgagcgccca ctacaagtac atgtgtaata gtgaagccac agatgtatta cacatgtact
      241 tgtagtggat gcctactgcc tcggaattca aggggctact ttaggagcaa ttatcttgtt
      301 tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg aattaaaatg
      361 gtataaatta aatcactttt ttcaattgga agactaatgc gtttaaactg tcgacctgca
      421 gccaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
      481 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
      541 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
      601 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
      661 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
      721 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc cgctgctgga
      781 caggggctcg gctgtgggca ctgacattcc gtggggtgtc
//
