LOCUS       sequence-254323-       13588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13588)
  AUTHORS   Luo JH, Fu ZY, Losi G, Kim BG, Prybylowski K, Vissel B, Vicini S
  TITLE     Functional expression of distinct NMDA channel subunits tagged with 
            green fluorescent protein in hippocampal neurons in culture.
  JOURNAL   Neuropharmacology. 2002 Mar . 42(3):306-18.
  PUBMED    11897109
REFERENCE   2  (bases 1 to 13588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuropharmacology. 2002 Mar . 42(3):306-18."
COMMENT     Sequence Label: pEGFP-NR2A
FEATURES             Location/Qualifiers
     source          1..13588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1206..1215
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1212..1214,1215..1217,1218..1928)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1212..1214,1215..1217,1218..1928)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1257..1278)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1518..1537)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1865..1886
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(9547..9565)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9548..9565)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     intron          9649..9714
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9844..9864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10136..10270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10173..10192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10227..10246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11121..11256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11751..11770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     rep_origin      complement(11883..12341)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12020..12041)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12235..12254
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(12465..12483)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12551..12655
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12656..12724,12725..13516)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12874..12893)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcc gttgccgtcg
      721 caggagcgct cagaggaccg gcagtcgctg tccggagtgg aacagaaagc tgagccaggg
      781 ctctagaaga gaggctcctg aggtgctgtg cctgagcatg gggctggatg aggtctgaga
      841 gtcgcggcgg cagcaatcag ccctggagat gaccaggggt ggccactgct gagaactatg
      901 tggagagagg ctgcgagccc tgctgcagag cctccggctg ggatagccgc cccccgtggg
      961 ggtgatgcgg acagcgcggg acagccaggg gagcgcgcgg gggccgcagc atgcgggaac
     1021 ccgctaaacc tggtggctgc tgaggcggcc gagatgctcg tgcgcgcagc acgccccatt
     1081 gcatcctcca ccttctccgg ctacagggac cctaagtggc gaccatgggc agattgggct
     1141 actggacctt gctggtattg ccggcccttc tggtctggcg cgatccggcg cagaccgccg
     1201 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1261 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1321 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     1381 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     1441 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1501 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1561 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1621 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     1681 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     1741 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1801 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1861 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     1921 tgtacaagtc cgcggcggcg gagaagggtc ctccagcgct gaacattgcg gtgctgctgg
     1981 gtcacagcca cgacgtgaca gaacgcgaac ttcgaaatct gtggggccca gagcaggcaa
     2041 ccggcttgcc cctggatgtg aacgtggtgg cgttattgat gaaccgcact gaccctaaga
     2101 gcctcatcac gcatgtgtgc gacctcatgt ccggggcgcg catccacggc ttggtgtttg
     2161 gagatgacac ggaccaggag gctgtggccc agatgctgga ttttatctcc tcacagactt
     2221 ttatccccat cttgggcatt catgggggtg catctatgat catggctgac aaggatccga
     2281 catccacgtt cttccagttt ggagcctcca tccagcagca agccacagtt atgctgaaga
     2341 tcatgcagga ctacgactgg cacgtcttct ccctggtcac caccatcttc cctggctacc
     2401 gagacttcat cagctttatc aagacaacag tggacaacag ctttgtgggc tgggatatgc
     2461 agaacgtgat cacactggac acctccttcg aggacgccaa gacgcaggtc cagctgaaga
     2521 agatccattc ttctgtcatc ctgctctact gctccaaaga cgaggctgtc ctcatcctga
     2581 gcgaggctcg ctccttcggc ctcactggct atgatttctt ctggattgtc cccagtttgg
     2641 tgtctgggaa cacagagctc atccccaaag agtttccatc aggtctcatt tcagtctctt
     2701 atgacgactg ggactacagc ctggaggcaa gagtgagaga cggtcttggg atcttaacca
     2761 ctgccgcatc ctccatgttg gagaagttct cctacattcc tgaggccaag gccagctgct
     2821 atgggcaggc agagaagcca gagaccccgc tacacaccct gcaccaattc atggtcaatg
     2881 tgacttggga tggcaaggac ttgtccttca ctgaggaagg ttaccaggtg caccccaggc
     2941 ttgtggtgat cgtgctgaac aaggaccggg agtgggaaaa ggtgggcaag tgggagaacc
     3001 agacgctgag cctgaggcac gctgtgtggc caaggtacaa gtccttttct gactgcgagc
     3061 cagatgacaa ccacctcagc attgtcacct tggaggaagc ccccttcgtc atcgtagagg
     3121 acatagaccc cctgactgag acctgtgtga ggaacacggt gccctgtcgg aagttcgtca
     3181 agatcaacaa ttcaaccaac gaagggatga atgtgaagaa atgctgcaag gggttctgca
     3241 tcgacatcct caagaagctg tccagaactg tgaagttcac ctatgacctc tacctggtga
     3301 ccaatgggaa gcatgggaaa aaggttaaca atgtgtggaa tggaatgata ggtgaagtgg
     3361 tctatcaacg agcagtcatg gctgtgggct cactcaccat caatgaggag cgttcggaag
     3421 tggtggactt ctcggtgccc tttgtggaga caggaatcag cgtcatggtc tccaggagta
     3481 atggcactgt ctccccttct gctttcctcg aacccttcag tgcctccgtc tgggtgatga
     3541 tgtttgtgat gctgctcatc gtctcagcca ttgctgtctt cgtttttgaa tacttcagtc
     3601 ctgttggata caacagaaac ttagccaaag ggaaagctcc ccacgggcct tcttttacta
     3661 ttggaaaagc tatatggctc ctctggggcc tggtcttcaa caattctgtg cctgtccaga
     3721 atcctaaagg cacaaccagc aagatcatgg tgtcagtgtg ggccttcttt gctgtcatct
     3781 tcctggccag ttacacagcc aacctggctg ccttcatgat ccaggaggag tttgtggacc
     3841 aagtgactgg cctcagtgac aagaagttcc agagacctca tgactattct ccacctttcc
     3901 gatttgggac ggtacccaat ggaagtacag agaggaatat tcgtaacaac tacccgtata
     3961 tgcaccagta catgaccaga ttcaaccaga ggggagtgga ggatgccttg gtcagcttga
     4021 aaaccgggaa gttggacgct ttcatctatg acgcagccgt cttgaactac aaggccggga
     4081 gggatgaagg ctgtaaactg gtgaccattg ggagcgggta catctttgct accacaggct
     4141 atggaattgc gctgcagaag ggctcaccct ggaagaggca gattgacctc gctctgctcc
     4201 agtttgttgg tgatggtgag atggaggagc tggagacact gtggcttacg ggcatctgcc
     4261 acaacgagaa gaatgaggtg atgagtagcc agctggacat cgataacatg gcgggcgtgt
     4321 tctacatgct ggctgcagcc atggccctca gcctcatcac cttcatctgg gagcacctct
     4381 tctactggaa gctgcgcttc tgcttcacag gcgtgtgctc tgaccggccc gggctgctct
     4441 tctccatcag caggggcatc tatagttgca tccatggggt acacattgaa gaaaagaaga
     4501 aatctccaga tttcaatctg actggatcac agagcaacat gctaaagctt cttcggtcag
     4561 ctaaaaacat ctccaatatg tccaacatga actcctcaag aatggactca cctaaaagag
     4621 ctactgactt cattcaaaga gggtcactta ttgtggacat ggtttcagac aagggaaatt
     4681 tgatatattc agacaacaga tcctttcaag ggaaggacag tatatttgga gacaacatga
     4741 atgaactcca aacatttgtg gccaacaggc acaaggataa tctcagtaac tatgtgtttc
     4801 aaggacagca tcctctcact ctcaatgagt ccaaccctaa cacagtagag gtggctgtca
     4861 gcactgaatc caaagggaac tcccgacccc ggcagctttg gaagaaatcc atggagtctc
     4921 tacgccagga ttctctaaac cagaacccag tctcccagag ggatgagaag actgcagaga
     4981 atcggaccca ctcgctaaag agtcctaggt atcttccaga agaggtagcc cactctgaca
     5041 tttcagaaac ctcaagccgg gccacatgcc acagggagcc agataacaat aagaaccaca
     5101 agaccaagga taacttcaaa cggtcaatgg cctctaagta tcccaaggac tgtagcgatg
     5161 ttgaccgcac ctacatgaaa accaaagcaa gttctcccag ggataagatc tataccattg
     5221 atggtgagaa ggagcccagc ttccacttag atcctcctca gtttgttgag aatataaccc
     5281 tgcctgagaa tgtgggcttc ccagatacct accaagatca caatgagaac ttccgcaagg
     5341 gggactccac actgcccatg aacaggaacc cattacataa tgaagacggg cttcccaaca
     5401 atgaccaata taaactctat gccaagcact ttaccttgaa agacaagggt tccccacaca
     5461 gtgagggcag tgatcgatac cggcagaact ccacacattg cagaagctgc ctttcgaatc
     5521 tgcccaccta ctcaggccac tttaccatga ggtctccttt caagtgtgat gcctgtctgc
     5581 ggatggggaa tctctatgac attgatgaag accagatgct tcaggagaca ggtaacccag
     5641 ctactcggga ggaggtctac cagcaggact ggtcacagaa caacgccctc cagttccaga
     5701 agaacaagct aaggattaac cgacagcact cctatgataa cattctggac aaacccagag
     5761 agatagacct tagcaggccc tcccggagca taagcctcaa ggacagggaa cggctactgg
     5821 agggcaactt gtatgggagc ctgttcagtg tcccctcaag caaactcttg gggaacaaaa
     5881 gctccctttt cccccaaggt ctggaggaca gcaagaggag caagtctctc ttgccagacc
     5941 acgcctccga taatcctttc ctccacacgt atggggatga ccaacgctta gttatcggga
     6001 gatgtccctc ggacccttac aaacactcat tgccatcaca ggcggtaaat gacagctatc
     6061 ttcggtcatc cttgaggtca acagcatcat attgctccag ggacagtcgg ggccacagtg
     6121 atgtgtatat ttcagagcat gttatgcctt atgctgcaaa taagaatacc atgtactcta
     6181 cccccagggt tttaaattcc tgcagcaata gacgagtgta caagaaaatg cctagtatcg
     6241 aatctgatgt ttaagatctt ccatcagtat ttatctataa ggaaacatat agaatggcca
     6301 acattataga gggtaaatgt tggatgtccg atagcaccct actaggagga agagggtaca
     6361 gggaggtact ttttgttggc tcttttgcac atggctccat gccataatct tccactcaag
     6421 gaatcttgtg agatatgtgc tgagcacagc atataccacg taggtgaatc cttaaccaaa
     6481 aacaaataaa tacacatggg caagtctccc agacatggcg actgggcacg gcggcaataa
     6541 tggtgcatca gacggcgatg gtgacattgt ggttgcctat attccaaatt ccatgaagat
     6601 caatccacca tgtaacttcc cccatcagaa atgcctcaca gtttctctaa tacagaataa
     6661 gcaatatggt atgcatgtaa gtctgacgca gacaataaga cagttaagaa tgcatctgca
     6721 ctgtagtaag attgacatgt gcaaggatta agaagtttgg cttgtaacag ttctcagatt
     6781 tcttgttatg ccttccatga caaccctgga tcagtccccc ttaaactcca ggttcgcccc
     6841 cccccccaaa gaatagcaaa tcagcgtgtt tgtggcaccc acactacttt cattgtaggc
     6901 ttcccccaag acacacctgg aacaaatagc cccagggact ctcaggtgga gaactgtaag
     6961 aatctctttg gatgttaatg tgtgtgtatc tctcaccctc agcttcattg gtcttgttcg
     7021 gagctgcata aacactttta tgtcttcgat atctaagtgt ggatcttctg tctatgacat
     7081 aatggctgtt gtagttaatc ttgaagactc atgtacgtaa gtctgcattt cctcccttct
     7141 ggtgatgact cagggttgta tagtatctct tacctgaccc ctcccagagt aacgtaactt
     7201 gttctgtccc cagacagcca tgatggtgtc taactagctg tgtgttgtgt tcctagagtt
     7261 gttaatgtgg tgacatgtgc cagcagccat gtgtaatctg taagaagtcc catgctatct
     7321 gtgtgtccaa ggctagcatg gttttacatt tatgtccctc aaagtcagga aactcttaga
     7381 caattatttc tatacaaacc tcctcaaatc aaaagccttc agacatcagc cacttgtgtg
     7441 tcccccccca agcacccttc caactgtgga taaactcagt aaaacaaggg atttctatcc
     7501 tctgaaatgt acaccttctt ggaggtgaat ttcttcccca gcaccaacag aaaccaatga
     7561 gaagtcttca acgcatttgt catttctgag ctgcctgcat tgtcctattc ttggcccact
     7621 gagcctgtgc atatatgaca gaacagatac tcattagatt aaaaaaaaga aaaactggag
     7681 ccaatgaggg gaatattcgt gcatggaaaa tgtcttagca cataattttt tatcacattt
     7741 ttaaaaagga ctcaaaaggt agttatgata ccttattaga caaggacatt cacaaaagca
     7801 gaaaataata tgaattaaac attttatatg cttcttttct agcaattagt atatttccaa
     7861 tgcattattg tccttgaaat agccaggctg ctgaccgtgg tatttcgcag gagactttag
     7921 ttggttgggt aactctctcc catgaaggat tctaaccatt tcttgttttt cctcttactg
     7981 aatcttggct tgggttcttg gagaaaggga tcgcacgaca ggttgaaatt tcatccctgt
     8041 tcttttcttc gtggtgttct attttatagg tgggattgtc caactacaag ttaagcaggg
     8101 aaggttctct tagagcttgg ggctgcttca gaaggagcta gcagagggaa agttcatttg
     8161 acctacagtg ttgcagaatg acaaaaagtg atgcaataca ccggaggacc agaggtggag
     8221 tggcagaggg ggtgaggatc tctcttcctg tgctcttgca gaacacagaa ggaggctggg
     8281 gtggagctag agggctgagg tcacaggact agggtggagc cagcggtctg gggcggagct
     8341 agaaggctga ggtcacagga ctagggtgga gccagtggtc tggggcggag ctagaaggct
     8401 ggggcggagc tagaagttga taagctttaa agtcacgttt tattccttgg acagagccat
     8461 cctttccttc cccaagtccc acagctgggg aaatgtgcat tagagaacct agaacagtct
     8521 ccctgatttt tgatcttaaa tttgagcttc agtgtgtgta gtgtcctttc acccctttta
     8581 ctttatttcc ccataggatt ccacacctgt attcctcctg ctaggactgc tgttgtgtcc
     8641 tcacagaact gactcttaag cctccctgga atacagaatc atgacttgtc acaaatctga
     8701 ggagcattct gctttcccgg attgtcctca aagcccaggg tgctgttgaa tgtcccacat
     8761 tttagtgaga gtacaaatgg catttatact gcctcctcaa aaccctctct ggaaaattca
     8821 ggatcatttc tgctattaac tctgtacaaa agaagctaaa atgaggcttt tggaagaatc
     8881 agtcacataa tgggaaagaa tacgtttgtt ttaacaccaa gatatttaca aaaataagtc
     8941 atggtagtta ggggctgtgt ggcaaacatt tgcttttccc tctcctctaa tttgctgcct
     9001 cttgcacatg ataacttcaa agaagaaaac gaggaggtca cgttccaagt gagaggtaat
     9061 catgtgggct tgtttgaagg aaagccaagt gagatccatc cttttcaaaa ctaaccccga
     9121 gggtggcaaa tggcacctac ggccggtgat gagtactgag caagaggcct tggcctgggt
     9181 atcacttaga gggttcccac actaaaccca acctctgagt ccttagactt acagatgcta
     9241 agaagccagt gtctcttaac tcaaaggcct gatctttccc tggactaagt cagggcagct
     9301 gccctgggca gttgtcttgg caattctttt gttttcctca ttttggaaag gaccagactg
     9361 gtcctcagat gtcaatactt gaaagcaaac agccatgcat ctgtccttgc acctgaaccg
     9421 gtgcaaatgc tgtgcacaag agaagctccc agagcatgac cttcactcgg ccgcgcgaca
     9481 gcaacggaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg
     9541 ccctattcta tagtgtcacc taaatgctag aggatctttg tgaaggaacc ttacttctgt
     9601 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     9661 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     9721 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     9781 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     9841 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     9901 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     9961 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc
    10021 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    10081 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    10141 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    10201 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10261 tgtctggatc gatcccgcca tggtatcaac gccatatttc tatttacagt agggacctct
    10321 tcgttgtgta ggtaccgctg tattcctagg gaaatagtag aggcaccttg aactgtctgc
    10381 atcagccata tagcccccgc tgttcgattt acaaacacag gcacagtact gacaaaccca
    10441 tacacctcct ctgaaatacc catagttgct agggctgtct ccgaactcat tacaccctcc
    10501 aaagtcagag ctgtaatttc gccatcaagg gcagcgaggg cttctccaga taaaatagct
    10561 tctgccgaga gtcccgtaag ggtagacact tcagctaatc cctcgatgag gtctactaga
    10621 atagtcagtg cggctcccat tttgaaaatt cacttacttg atcagcttca gaagatggcg
    10681 gagggcctcc aacacagtaa ttttcctccc gactcttaaa atagaaaatg tcaagtcagt
    10741 taagcaggaa gtgactaact gaccgcagct ggccgtgcga catcctcttt taattagttg
    10801 ctaggcaact gccctccaga gggcagtgtg gttttgcaag aggaagcaaa aagcctctcc
    10861 acccaggcct agaatgtttc cacccaatca ttactatgac aacagctgtt ttttttagta
    10921 ttaagcagag gccgggggcc cctggcctcc gcttactctg gagaaaaaga agagaggcat
    10981 tgtagaggct tccagaggca acttgtcaaa acaggactgc ttctatttct gtcacactgt
    11041 ctggccctgt cacaaggtcc agcacctcca tacccccttt aataagcagt ttgggaacgg
    11101 gtgcgggtct tactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    11161 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
    11221 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaattc
    11281 ggcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    11341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    11401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    11461 cctacatacc tcgctctgct gaagccagtt accagtggct gctgccagtg gcgataagtc
    11521 gtgtcttacc gggttggact caagagatag ttaccggata aggcgcagcg gtcgggctga
    11581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    11641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcaagcta gcttctagct
    11881 agaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
    11941 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
    12001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    12061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc cgcccactac gtgaaccatc
    12121 acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
    12181 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
    12241 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
    12301 caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt gctttgacga
    12361 gcacgtataa cgtgctttcc tcgttagaat cagagcggga gctaaacagg aggccgatta
    12421 aagggatttt agacaggaac ggtacgccag ctggacccga aagggcctcg tgatacgcct
    12481 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    12541 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    12601 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    12661 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    12721 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    12781 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    12841 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
    12901 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    12961 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    13021 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    13081 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    13141 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
    13201 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    13261 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    13321 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    13381 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    13441 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    13501 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    13561 acttcatttt taatttctct agcgcgtt
//
