LOCUS       sequence-25433-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: pLB2 CAG P2Gm
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           63..184
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           211..225
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           253..379
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(518..538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 agctgtacnn gtagcggccg caagccttgt taagtgctcg cttcggcagc acatatacta
       61 tgttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
      121 tgggattact tcttcaggtt aacccaacag aaggctcgag aaggtatatt gctgttgaca
      181 gtgagcgagc tcccgtgaat tggaatccta gtgaagccac agatgtagga ttccaattca
      241 gcgggagcct gcctactgcc tcggaattca aggggctact ttaggagcaa ttatcttgtt
      301 tactaaaact gaataccttg ctatctcttt gatacatttt tacaaagctg aattaaaatg
      361 gtataaatta aatcactttt ttcaattgga agactaatgc gtttaaacca catgtttaag
      421 ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt
      481 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
      541 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
      601 cctccttgta taaatcctg
//
