LOCUS       sequence-254334-       11035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-ON inducible lentivirus expressing mcherry-labeled DN 
            KASH-Delta-PPPL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11035)
  AUTHORS   Zhang Q, Narayanan V, Mui KL, O'Bryan CS, Anderson RH, Kc B, Cabe 
            JI, Denis KB, Antoku S, Roux KJ, Dickinson RB, Angelini TE, 
            Gundersen GG, Conway DE, Lele TP
  TITLE     Mechanical Stabilization of the Glandular Acinus by Linker of 
            Nucleoskeleton and Cytoskeleton Complex.
  JOURNAL   Curr Biol. 2019 Aug 2. pii: S0960-9822(19)30866-8. doi: 
            10.1016/j.cub.2019.07.021.
  PUBMED    31402305
REFERENCE   2  (bases 1 to 11035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2019 Aug 2. pii: S0960-9822(19)30866-8. doi: 
            10.1016/j.cub.2019.07.021."
COMMENT     Sequence Label: pinducer 20 DN-KASH-Delta-PPPL
FEATURES             Location/Qualifiers
     source          1..11035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2230..2248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2314..2332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2398..2416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2440..2458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2491..2529
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2526..2550
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2659..2683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2692..2701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2698..3405
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2843..2861)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3111..3130
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(3608..3632)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3685..3711
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3797..5008
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4845..4864
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5067..5684,5685..5690,5691..5726,5727..5729,5730..5765,
                     5766..5768,5769..5804,5805..5813)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5865..6438
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6032..6049)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6259..6278
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6454..7248
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6454..7248
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6508..6527)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7118..7137
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7425..8013
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7478..7498)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7896..7907)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8088..8321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8440..8458)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8526..8630
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8631..8699,8700..9491)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8849..8868)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9662..10250
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10151..10170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10404..10421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10661..10682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10697..10702,10703..10720,10721..10727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10735..10751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10740..10762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10759..10775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10759..10775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10825..10844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga ggcctatata
     2521 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2581 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt
     2641 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gcttcaagct tgccaccatg
     2701 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     2761 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     2821 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     2881 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     2941 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     3001 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     3061 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     3121 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     3181 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     3241 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     3301 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     3361 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagggagg aggaggagga
     3421 ggaggaggac gcggcttcct gttcagagtc ctccgagcag ctcttcccct tcagcttctc
     3481 ctgctcctcc tcatcgggct tgcctgcctt gtaccaatgt cagaggaaga ctacagctgt
     3541 gccctctcca acaactttgc ccggtcattc caccccatgc tcagatacac gaatggctga
     3601 gaattcgacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     3661 agtctagagg gcccgcggtt cgaataccca tacgacgtcc cagactacgc ttagtaatga
     3721 ttaattaaac tagaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagt
     3781 aacccaccca agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     3841 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     3901 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     3961 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     4021 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     4081 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     4141 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     4201 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     4261 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     4321 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     4381 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     4441 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     4501 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     4561 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     4621 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     4681 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     4741 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     4801 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     4861 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     4921 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     4981 ctggccgttt ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc
     5041 gtaccggtcc tgcagtcgaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg
     5101 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     5161 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     5221 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     5281 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     5341 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     5401 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     5461 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     5521 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     5581 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     5641 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     5701 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     5761 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     5821 aggatccgcg gccgcactag aggaattccg cccctctccc cccccccctc tccctccccc
     5881 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     5941 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     6001 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     6061 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     6121 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     6181 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     6241 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     6301 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     6361 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     6421 tgaaaaacac gatgataagc ttaccggtcc accatgattg aacaagatgg attgcacgca
     6481 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     6541 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     6601 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg
     6661 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6721 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6781 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6841 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6901 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6961 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc
     7021 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     7081 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     7141 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     7201 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgatg tacaagtaaa
     7261 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7321 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7381 ttagtccctc ccaattcgat atcaagctta tcgcgataga tcctaatcaa cctctggatt
     7441 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7501 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7561 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7621 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7681 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7741 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7801 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     7861 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7921 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7981 cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta
     8041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8101 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8281 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8341 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt
     8401 aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     8461 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     8521 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8581 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8641 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8761 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8821 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     8881 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9181 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9241 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9481 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9541 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9601 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9661 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9721 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9781 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9841 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9901 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9961 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10021 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10081 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10141 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10201 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10261 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10321 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10381 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10441 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
    10501 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    10561 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
    10621 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    10681 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    10741 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
    10801 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10861 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    10921 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    10981 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
