LOCUS       sequence-25434-e         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Stern P, Astrof S, Erkeland SJ, Schustak J, Sharp PA, Hynes RO
  TITLE     A system for Cre-regulated RNA interference in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13895-900.
  PUBMED    18779577
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Sep 16. 105(37):13895-900."
COMMENT     Sequence Label: pLB2 Ubi-FLIP FF
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           61..182
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           209..223
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           251..377
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    407..440
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ctgtacaagt agcggccgca agccttgtta agtgctcgct tcggcagcac atatactatg
       61 ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa acacttgctg
      121 ggattacttc ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc tgttgacagt
      181 gagcgagctc ccgtgaattg gaatcctagt gaagccacag atgtaggatt ccaattcagc
      241 gggagcctgc ctactgcctc ggaattcaag gggctacttt aggagcaatt atcttgttta
      301 ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt
      361 ataaattaaa tcactttttt caattggaag actaatgcgt ttaaacataa cttcgtataa
      421 tgtgtactat acgaagttat ggcttctaga ctccagagaa atgaagtcca gggcttggag
      481 gagggagagg gaaagcagca gcagcagcat ccaggatgtg ttctgaacca gcaggcttat
      541 gccgccacac ttgaccagct tgtctctata cacactgata cttttattgg agctcatggg
      601 attcgcgccc gagacgcgaa gctcacaaaa gtagtcgccc tcatccttgg tggtgaagtt
      661 ggctagggta aggaccttga tatagggctg gttggagagg gtgacgcggg agcggtacgt
      721 gtgctc
//
