LOCUS       sequence-254342-       13606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13606)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 13606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: AcGFP-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..13606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1269
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     protein_bind    1336..1360
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10176..10200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10334..10455
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10371..10390)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10425..10444
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(10462..10917)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10599..10620)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10811..10830
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10944..11048
                     /gene="bla"
                     /label=AmpR promoter
     promoter        11050..11407
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11050..11241
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11073..11093)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      11258..11393
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11442..12236
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11442..12236
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11496..11515)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12106..12125
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(12424..12443)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12468..12515
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12844..13432
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13333..13352
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgcc gagctgttca ccggcatcgt gcccatcctg
      601 atcgagctga atggcgatgt gaatggccac aagttcagcg tgagcggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg
      721 ccctggccca ccctggtgac caccctgagc tacggcgtgc agtgcttctc acgctacccc
      781 gatcacatga agcagcacga cttcttcaag agcgccatgc ctgagggcta catccaggag
      841 cgcaccatct tcttcgagga tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag
      901 ggcgataccc tggtgaatcg catcgagctg accggcaccg atttcaagga ggatggcaac
      961 atcctgggca ataagatgga gtacaactac aacgcccaca atgtgtacat catgaccgac
     1021 aaggccaaga atggcatcaa ggtgaacttc aagatccgcc acaacatcga ggatggcagc
     1081 gtgcagctgg ccgaccacta ccagcagaat acccccatcg gcgatggccc tgtgctgctg
     1141 cccgataacc actacctgtc cacccagagc gccctgtcca aggaccccaa cgagaagcgc
     1201 gatcacatga tctacttcgg cttcgtgacc gccgccgcca tcacccacgg catggatgag
     1261 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg
     1321 taccgcgggc ccatcacaag tttgtacaaa aaagcaggct ccgaattcgc ccttatggct
     1381 agtggcagct gtcaggggtg cgaagaggac gaggaaactc tgaagaagtt gatagtcagg
     1441 ctgaacaatg tccaggaagg aaaacagata gaaacgctgg tccaaatcct ggaggatctg
     1501 ctggtgttca cgtactccga gcacgcctcc aagttatttc aaggcaaaaa tatccatgtg
     1561 cctctgttga tcgtcttgga ctcctatatg agagtcgcga gtgtgcagca ggtgggttgg
     1621 tcacttctgt gcaaattaat agaagtctgt ccaggtacaa tgcaaagctt aatgggaccc
     1681 caggatgttg gaaatgattg ggaagtcctt ggtgttcacc aattgattct taaaatgcta
     1741 acagttcata atgccagtgt aaacttgtca gtgattggac tgaagacctt agatctcctc
     1801 ctaacttcag gtaaaatcac cttgctgata ttggatgaag aaagtgatat tttcatgtta
     1861 atttttgatg ccatgcactc atttccagcc aatgatgaag tccagaaact tggatgcaaa
     1921 gctttacatg tgctgtttga gagagtctca gaggagcaac tgactgaatt tgttgagaac
     1981 aaagattata tgatattgtt aagtgcgtta acaaatttta aagatgaaga ggaaattgtg
     2041 cttcatgtgc tgcattgttt acattcccta gcgattcctt gcaataatgt ggaagtcctc
     2101 atgagtggca atgtcaggtg ttataatatt gtggtggaag ctatgaaagc attccctatg
     2161 agtgaaagaa ttcaagaagt gagttgctgt ttgctccata ggcttacatt aggtaatttt
     2221 ttcaatatcc tggtattaaa cgaagtccat gagtttgtgg tgaaagctgt gcagcagtac
     2281 ccagagaatg cagcattgca gatctcagcg ctcagctgtt tggccctcct cactgagact
     2341 attttcttaa atcaagattt agaggaaaag aatgagaatc aagagaatga tgatgagggg
     2401 gaagaagata aattgttttg gctggaagcc tgttacaaag cattaacgtg gcatagaaag
     2461 aacaagcacg tgcaggaggc cgcatgctgg gcactaaata atctccttat gtaccaaaac
     2521 agtttacatg agaagattgg agatgaagat ggccatttcc cagctcatag ggaagtgatg
     2581 ctctccatgc tgatgcattc ttcatcaaag gaagttttcc aggcatctgc gaatgcattg
     2641 tcaactctct tagaacaaaa tgttaatttc agaaaaatac tgttatcaaa aggaatacac
     2701 ctgaatgttt tggagttaat gcagaagcat atacattctc ccgaagtggc tgaaagtggc
     2761 tgtaaaatgc taaatcatct ttttgaagga agcaacactt ccctggatat aatggcagca
     2821 gtggtcccca aaatactaac agttatgaaa cgtcatgaga catcattacc agtgcagctg
     2881 gaggcgcttc gagctatttt acattttata gtgcctggca tgccagaaga atccagggag
     2941 gatacagaat ttcatcataa gctaaatatg gttaaaaaac agtgtttcaa gaatgatatt
     3001 cacaaactgg tcctagggaa ggtgcgaata agcggggaaa ttcttctcgg ctgactcagt
     3061 catttcattt cttcatgttt gagccgattt tttctcccgt aaatgccttg aatcagccta
     3121 tttagaccgt ttcttcgcca tttaaggcgt tatccccagt ttttagtgag atctctccca
     3181 ctgacgtatc atttggtccg cccgaaacag gttggccagc gtgaataaca tcgccagttg
     3241 gttatcgttt ttcagcaacc ccttgtatct ggctttcacg aagccgaact gtcgcttgat
     3301 gatgcgaaat gggtgctcca ccctggcccg gatgctggct ttcatgtatt cgatgttgat
     3361 ggccgttttg ttcttgcgtg gatgctgttt caaggttctt accttgccgg ggcgctcggc
     3421 gatcagccag tccacatcca cctcggccag ctcctcgcgc tgtggcgccc cttggtagcc
     3481 ggcatcggct gagacaaatt gctcctctcc atgcagcaga ttacccagct gattgaggtc
     3541 atgctcgttg gccgcggtgg tgaccaggct gtgggtcagg ccactcttgg catcgacacc
     3601 aatgtgggcc ttcatgccaa agtgccactg attgcctttc ttggtctgat gcatctccgg
     3661 atcgcgttgc tgctctttgt tcttggtcga gctgggtgcc tcaatgatgg tggcatcgac
     3721 caaggtgcct tgagtcatca tgacgcctgc ttcggccagc cagcgattga tggtcttgaa
     3781 caattggcgg gccagttgat gctgctccag caggtggcgg aaattcatga tggtggtgcg
     3841 gtccggcaag gcgctatcca gggataaccg ggcaaacaga cgcatggagg cgatttcgta
     3901 cagagcatct tccatcgcgc catcgctcag gttgtaccaa tgctgcatgc agtgaatgcg
     3961 tagcatggtt tccagcggat aaggtcgccg gccattacca gccttggggt aaaacggctc
     4021 gatgacttcc accatgtttt gccatggcag aatctgctcc atgcgggaca agaaaatctc
     4081 ttttctggtc tgacggcgct tactgctgaa ttcactgtcg gcgaaggtaa gttgatgact
     4141 catgatgaac cctgttctat ggctccagat gacaaacatg atctcatatc agggacttgt
     4201 tcgcaccttc cctagcagct ttgaacaggt tcattggaaa tcctgggatt cagaaatgtg
     4261 gattaaaagt aatttcttct attgtacatt ttcctgatgc attagagatg ttatccctgg
     4321 aaggtgctat ggattcagtg cttcacacac tgcagatgta tccagatgac caagaaattc
     4381 agtgtctggg tttaagtctt ataggatact tgattacaaa gaagaatgtg ttcataggaa
     4441 ctggacatct gctggcaaaa attctggttt ccagcttata ccgatttaag gatgttgctg
     4501 aaatacagac taaaggattt cagacaatct tagcaatcct caaattgtca gcatcttttt
     4561 ctaagctgct ggtgcatcat tcatttgact tagtaatatt ccatcaaatg tcttccaata
     4621 tcatggaaca aaaggatcaa cagtttctaa acctctgttg caagtgtttt gcaaaagtag
     4681 ctatggatga ttacttaaaa aatgtgatgc tagagagagc gtgtgatcag aataacagca
     4741 tcatggttga atgcttgctt ctattgggag cagatgccaa tcaagcaaag gagggatctt
     4801 ctttaatttg tcaggtatgt gagaaagaga gcagtcccaa attggtggaa ctcttactga
     4861 atagtggatc tcgtgaacaa gatgtacgaa aagcgttgac gataagcatt gggaaaggtg
     4921 acagccagat catcagcttg ctcttaagga ggctggccct ggatgttgcc aacaatagca
     4981 tttgccttgg aggattttgt ataggaaaag ttgaaccttc ttggcttggt cctttatttc
     5041 cagataagac ttctaattta aggaaacaaa caaatatagc atctacacta gcaagaatgg
     5101 tgatcagata tcagatgaaa agtgctgtgg aagaaggaac agcctcaggc agcgatggaa
     5161 atttttctga agatgtgctg tctaaatttg atgaatggac ctttattcct gactcttcta
     5221 tggacagtgt gtttgctcaa agtgatgacc tggatagtga aggaagtgaa ggctcatttc
     5281 ttgtgaaaaa gaaatctaat tcaattagtg taggagaatt ttaccgagat gccgtattac
     5341 agcgttgctc accaaatttg caaagacatt ccaattcctt ggggcccatt tttgatcatg
     5401 aagatttact gaagcgaaaa agaaaaatat tatcttcaga tgattcactc aggtcatcaa
     5461 aacttcaatc ccatatgagg cattcagaca gcatttcttc tctggcttct gagagagaat
     5521 atattacatc actagacctt tcagcaaatg aactaagaga tattgatgcc ctaagccaga
     5581 aatgctgtat aagtgttcat ttggagcatc ttgaaaagct ggagcttcac cagaatgcac
     5641 tcacgagctt tccacaacag ctatgtgaaa ctctgaagag tttgacacat ttggacttgc
     5701 acagtaataa atttacatca tttccttctt atttgttgaa aatgagttgt attgctaatc
     5761 ttgatgtctc tcgaaatgac attggaccct cagtggtttt agatcctaca gtgaaatgtc
     5821 caactctgaa acagtttaac ctgtcatata accagctgtc ttttgtacct gagaacctca
     5881 ctgatgtggt agagaaactg gagcagctca ttttagaagg aaataaaata tcagggatat
     5941 gctccccctt gagactgaag gaactgaaga ttttaaacct tagtaagaac cacatttcat
     6001 ccctatcaga gaactttctt gaggcttgtc ctaaagtgga gagtttcagt gccagaatga
     6061 attttcttgc tgctatgcct ttcttgcctc cttctatgac aatcctaaaa ttatctcaga
     6121 acaaattttc ctgtattcca gaagcaattt taaatcttcc acacttgcgg tctttagata
     6181 tgagcagcaa tgatattcag tacctaccag gtcccgcaca ctggaaatct ttgaacttaa
     6241 gggaactctt atttagccat aatcagatca gcatcttgga cttgagtgaa aaagcatatt
     6301 tatggtctag agtagagaaa ctgcatcttt ctcacaataa actgaaagag attcctcctg
     6361 agattggctg tcttgaaaat ctgacatctc tggatgtcag ttacaacttg gaactaagat
     6421 cctttcccaa tgaaatgggg aaattaagca aaatatggga tcttcctttg gatgaactgc
     6481 atcttaactt tgattttaaa catataggat gtaaagccaa agacatcata aggtttcttc
     6541 aacagcgatt aaaaaaggct gtgccttata accgaatgaa acttatgatt gtgggaaata
     6601 ctgggagtgg taaaaccacc ttattgcagc aattaatgaa aaccaagaaa tcagatcttg
     6661 gaatgcaaag tgccacagtt ggcatagatg tgaaagactg gcctatccaa ataagagaca
     6721 aaagaaagag agatctcgtc ctaaatgtgt gggattttgc aggtcgtgag gaattctata
     6781 gtactcatcc ccattttatg acgcagcgag cattgtacct tgctgtctat gacctcagca
     6841 agggacaggc tgaagttgat gccatgaagc cttggctctt caatataaag gctcgcgctt
     6901 cttcttcccc tgtgattctc gttggcacac atttggatgt ttctgatgag aagcaacgca
     6961 aagcctgcat gagtaaaatc accaaggaac tcctgaataa gcgagggttc cctgccatac
     7021 gagattacca ctttgtgaat gccaccgagg aatctgatgc tttggcaaaa cttcggaaaa
     7081 ccatcataaa cgagagcctt aatttcaaga tccgagatca gcttgttgtt ggacagctga
     7141 ttccagactg ctatgtagaa cttgaaaaaa tcattttatc ggagcgtaaa aatgtgccaa
     7201 ttgaatttcc cgtaattgac cggaaacgat tattacaact agtgagagaa aatcagctgc
     7261 agttagatga aaatgagctt cctcacgcag ttcactttct aaatgaatca ggagtccttc
     7321 ttcattttca agacccagca ctgcagttaa gtgacttgta ctttgtggaa cccaagtggc
     7381 tttgtaaaat catggcacag attttgacag tgaaagtgga aggttgtcca aaacacccta
     7441 agggaattat ttcgcgtaga gatgtggaaa aatttctttc aaaaaaaagg aaatttccaa
     7501 agaactacat gtcacagtat tttaagctcc tagaaaaatt ccagattgct ttgccaatag
     7561 gagaagaata tttgctggtt ccaagcagtt tgtctgacca caggcctgtg atagagcttc
     7621 cccattgtga gaactctgaa attatcatcc gactatatga aatgccttat tttccaatgg
     7681 gattttggtc aagattaatc aatcgattac ttgagatttc accttacatg ctttcaggga
     7741 gagaacgagc acttcgccca aacagaatgt attggcgaca aggcatttac ttaaattggt
     7801 ctcctgaagc ttattgtctg gtaggatctg aagtcttaga caatcatcca gagagtttct
     7861 taaaaattac agttccttct tgtagaaaag gctgtattct tttgggccaa gttgtggacc
     7921 acattgattc tctcatggaa gaatggtttc ctgggttgct ggagattgat atttgtggtg
     7981 aaggagaaac tctgttgaag aaatgggcat tatatagttt taatgatggc gaagaacatc
     8041 aaaaaatctt acttgatgac ttgatgaaga aagcagagga aggagatctc ttagtaaatc
     8101 cagatcaacc aaggctcacc attccaatat ctcagattgc ccctgacttg attttggctg
     8161 acctgcctag aaatattatg ttgaataatg atgagttgga atttgaacaa gctccagagt
     8221 ttctcctagg tgatggcagt tttggatcag tttaccgagc agcctatgaa ggagaagaag
     8281 tggctgtgaa gatttttaat aaacatacat cactcaggct gttaagacaa gagcttgtgg
     8341 tgctttgcca cctccaccac cccagtttga tatctttgct ggcagctggg attcgtcccc
     8401 ggatgttggt gatggagtta gcctccaagg gttccttgga tcgcctgctt cagcaggaca
     8461 aagccagcct cactagaacc ctacagcaca ggatcgcact ccacgtagct gatggtttga
     8521 gatacctcca ctcagccatg attatatacc gagacctgaa accccacaat gtgctgcttt
     8581 tcacactgta tcccaatgct gccatcatcg caaagattgc tgactacggc attgctcagt
     8641 actgctgtag aatggggata aaaacatcag agggcacacc agggtttcgt gcacctgaag
     8701 ttgccagagg aaatgtcatt tataaccaac aggctgatgt ttattcattt ggtttactac
     8761 tctatgacat tttgacaact ggaggtagaa tagtagaggg tttgaagttt ccaaatgagt
     8821 ttgatgaatt agaaatacaa ggaaaattac ctgatccagt taaagaatat ggttgtgccc
     8881 catggcctat ggttgagaaa ttaattaaac agtgtttgaa agaaaatcct caagaaaggc
     8941 ctacttctgc ccaggtcttt gacattttga attcagctga attagtctgt ctgacgagac
     9001 gcattttatt acctaaaaac gtaattgttg aatgcatggt tgctacacat cacaacagca
     9061 ggaatgcaag catttggctg ggctgtgggc acaccgacag aggacagctc tcatttcttg
     9121 acttaaatac tgaaggatac acttctgagg aagttgctga tagtagaata ttgtgcttag
     9181 ccttggtgca tcttcctgtt gaaaaggaaa gctggattgt gtctgggaca cagtctggta
     9241 ctctcctggt catcaatacc gaagatggga aaaagagaca taccctagaa aagatgactg
     9301 attctgtcac ttgtttgtat tgcaattcct tttccaagca aagcaaacaa aaaaattttc
     9361 ttttggttgg aaccgctgat ggcaagttag caatttttga agataagact gttaagctta
     9421 aaggagctgc tcctttgaag atactaaata taggaaatgt cagtactcca ttgatgtgtt
     9481 tgagtgaatc cacaaattca acggaaagaa atgtaatgtg gggaggatgt ggcacaaaga
     9541 ttttctcctt ttctaatgat ttcaccattc agaaactcat tgagacaaga acaagccaac
     9601 tgttttctta tgcagctttc agtgattcca acatcataac agtggtggta gacactgctc
     9661 tctatattgc taagcaaaat agccctgttg tggaagtgtg ggataagaaa actgaaaaac
     9721 tctgtggact aatagactgc gtgcactttt taagggaggt aatggtaaaa gaaaacaagg
     9781 aatcaaaaca caaaatgtct tattctggga gagtgaaaac cctctgcctt cagaagaaca
     9841 ctgctctttg gataggaact ggaggaggcc atattttact cctggatctt tcaactcgtc
     9901 gacttatacg tgtaatttac aacttttgta attcggtcag agtcatgatg acagcacagc
     9961 taggaagcct taaaaatgtc atgctggtat tgggctacaa ccggaaaaat actgaaggta
    10021 cacaaaagca gaaagagata caatcttgct tgaccgtttg ggacatcaat cttccacatg
    10081 aagtgcaaaa tttagaaaaa cacattgaag tgagaaaaga attagctgaa aaaatgagac
    10141 gaacatctgt tgagtaagag agaagggcga attcgaccca gctttcttgt acaaagtggt
    10201 gatgggatcc accggatcta gataactgat cataatcagc cataccacat ttgtagaggt
    10261 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    10321 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    10381 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10441 catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
    10501 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
    10561 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    10621 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    10681 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    10741 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    10801 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    10861 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
    10921 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    10981 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11041 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
    11101 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    11161 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    11221 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    11281 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    11341 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    11401 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
    11461 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    11521 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
    11581 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
    11641 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    11701 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    11761 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    11821 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    11881 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    11941 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
    12001 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    12061 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    12121 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    12181 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    12241 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    12301 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    12361 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
    12421 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
    12481 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
    12541 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
    12601 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
    12661 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
    12721 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    12781 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    12841 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    12901 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    12961 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    13021 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    13081 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    13141 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    13201 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    13261 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    13321 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    13381 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    13441 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    13501 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
    13561 aatcaattac ggggtcatta gttcatagcc catatatgga gttccg
//
