LOCUS       sequence-25435-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Yao T, Song L, Xu W, DeMartino GN, Florens L, Swanson SK, Washburn 
            MP, Conaway RC, Conaway JW, Cohen RE
  TITLE     Proteasome recruitment and activation of the Uch37 deubiquitinating 
            enzyme by Adrm1.
  JOURNAL   Nat Cell Biol. 2006 Sep . 8(9):994-1002.
  PUBMED    16906146
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2006 Sep . 8(9):994-1002."
COMMENT     Sequence Label: pcDNA3-HA-Uch37
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             114..140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             144..170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcnttagcga gctctctggc tactagagaa cccactgctt actggcttat cgaaattaat
       61 acgactcact atagggagac ccaagcttgg taccgctagc cgccaccatg gcttacccat
      121 acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca ggatccatga
      181 cgggcaacgc cggggagtgg tgcctcatgg aaagcgaccc cggggtcttc accgagctca
      241 ttaaaggatt cggttgccga ggagcccaag tagaagaaat atggagttta gagcctgaga
      301 attttgaaaa attaaagcca gttcatgggt taatttttct tttcaagtgg cagccaggag
      361 aagaaccagc aggctctgtg gttcaggact cccgacttga cacgatattt tttgctaagc
      421 aggtaattaa taatgcttgt gctactcaag ccatagtgag tgtgttactg aactgtaccc
      481 accaggatgt ccatttaggc gagacattat cagagtttaa agaattttca caaagttttg
      541 atgcagctat gaaaggcttg gcactgagca attcagatgt gattcgacaa gtacacaaca
      601 gtttcgccag acagcaaatg tttgaatttg atacgaagac atcagcaaaa gaagaagatg
      661 cttttcactt tgtcagttat gttcctgtta atgggagact gtatgaatta gatggattaa
      721 gagaaggacc gattgattta ggtgcatgca nannaangtt ggcnncgcgn ncggcgcccn
      781 cnnaaaaaaa aangggatnc aaagtccngg ggngggnaat ncgntttttt tangg
//
