LOCUS       sequence-254360-        5813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS-driven expression of Positron in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdelfattah AS, Valenti R, Zheng J, Wong A, Podgorski K, Koyama M, 
            Kim DS, Schreiter ER
  TITLE     A general approach to engineer positive-going eFRET voltage 
            indicators.
  JOURNAL   Nat Commun. 2020 Jul 10;11(1):3444. doi: 10.1038/s41467-020-17322-1.
  PUBMED    32651384
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-10"; volume: "11(1):3444. doi"; pages: " 
            10.1038/s41467-020-17322-1"
COMMENT     Sequence Label: pT2-Tbait-UAS-Positron-ST
FEATURES             Location/Qualifiers
     source          1..5813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(17..28,29..191)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        635..653
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    2911..3045
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2935..2954)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2989..3008
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(3453..3640,3641..3652)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3686..3702)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3686..3702)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3739..3757)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3778..3794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3778..3794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3791..3813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3802..3818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3826..3832,3833..3850,3851..3856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4009..4026)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4180..4768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4260..4279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4939..5730,5731..5799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5562..5581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggcatatggt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta
       61 caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact
      121 tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt
      181 aaaagcaaga aagaaaacta gagattcttg tttaagcttt taatctcaaa aaacattaaa
      241 tgaaatgcat acaaggtttt atcctgcttt agaactgttt gtatttaatt atcaaactat
      301 aagacagaca atctaatgcc agtacacgct actcaaagtt gtaaaacctc agatttaact
      361 tcagtagaag ctgattctca aaattgttag tgtcaagcct agctcttttg gggctgaaaa
      421 gcaatcctgc agtgctgaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag
      481 tcttgataga gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa
      541 cacaggccag atgggccctc gacggtatcg ataagaaaac tagagattct tgtttaagct
      601 tgaggctgct gtcagggagc tcatggactt cgagaattaa ccctcactaa agggaatacg
      661 aagttatccg gagtactgtc ctccgacact agaggtcgga gtactgtcct ccgacgcaag
      721 gcggagtact gtcctccgac gcaaggtcgg agtactgtcc tccgacacta gaggtcggag
      781 tactgtcctc cgccatactt atccggagta ctgtcctccg acactagagg tcggagtact
      841 gtcctccgac gcaaggcgga gtactgtcct ccgacgcaag gtcggagtac tgtcctccga
      901 cactagaggt cggagtactg tcctccgcca tacgtagcag ggtagactgt agagggtata
      961 taatggaacc catcgcgtct cagcctcact ttgagctcct ccacacgaat tcgccatcat
     1021 ggctgacgtg gaaaccgaga ccggtatgat tgcacagtgg attgtctttg ctattatggc
     1081 tgctgctgct attgcttttg gagtggctgt gcactttcgg ccttcagagc tgaagagcgc
     1141 atactatatc aacattgcca tctgcactat cgccgctacc gcttactatg caatggccgt
     1201 gaactaccag gacctgacaa tgaatggtga aaggcaggtg gtctacgcaa gatatattga
     1261 ctgggtgctg accacaccac tgctcctgct caacctcatc gtcatgacca agatgggcgg
     1321 agtgatgatt tcttgggtca tcggcgcaga cattttcatg atcgtgtttg gtattctggg
     1381 cgccttcgag gatgaacaca agttcaaatg ggtgtacttt atcgctggat gtgtgatgca
     1441 ggcagtcctg acatacggga tgtataacgc cacttggaaa gacgatctga agaaaagccc
     1501 cgagtaccat agctcctatg tcagtctgct cgtcttcctg tcaatcctct gggtgtttta
     1561 tcctgtcgtg tgggctttcg ggtctggtag tggcgtgctg tccgtcgaca atgtggccat
     1621 tctcatggga atcctggatg tgctcgctaa gccactgttt ggaatggggt gcctcattgc
     1681 ccatgagact atcttcaaga tcggtaccgg ctttccattc gacccccatt atgtggaagt
     1741 cctgggcgag cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt
     1801 cctgcacggt aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc
     1861 gacccatcgc tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct
     1921 gggttatttc ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct
     1981 ggaagaggtc gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa
     2041 gcgcaatcca gagcgcgtca aaggtattgc atttatggag ttcatccacc ctatcccgac
     2101 ctgggacgaa tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt
     2161 cggccgcaag ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt
     2221 cgtccgcccg ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaatcctgt
     2281 tgaccgcgag ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa
     2341 catcgtcgcg ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct
     2401 gctgttctgg ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa
     2461 aagcctgcct aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga
     2521 caacccggac ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg
     2581 cgagccaacc actaagagca ggatcaccag cgagggcgag tacatccccc tggaccagat
     2641 cgacatcaac gtgttctgct acgagaacga ggtgcaaagt cagcctatcc tgaacacaaa
     2701 ggaaatggct ccacagtcta agcctcccga agagcttgag atgtccagta tgccaagtcc
     2761 cgtggctccc ctccctgcca ggactgaagg agtgattgac atgaggagta tgtcatctat
     2821 tgatagcttc atctcttgcg caacagattt ccccgaggct actcgattct aaactcgagc
     2881 ctctagaact atagtgagtc gtattacgta gatccagaca tgataagata cattgatgag
     2941 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     3001 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     3061 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt cgcggccgcg
     3121 gatccagatc gatctgcgaa gatacggcca cgggtgctct tgatcctgtg gctgattttg
     3181 gactgtgctg ctcgcagctg ctgatgaatc acatacttcc tccattttct tccactgatt
     3241 gactgttata atttccctaa tttccaggtc aaggtgctgt gcattgtggt aatagatgtg
     3301 acatgacgtc acttccaaag gaccaatgaa catgtctgac caatttcata taatgtgaaa
     3361 acgattttca taggcagaat aaataacatt taaattaaac tgggcatcag cgcaattcaa
     3421 ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt
     3481 tgactgtaaa taaaattgta aggagtaaaa agtacttttt tttctaaaaa aatgtaatta
     3541 agtaaaagta aaagtattga tttttaattg tactcaagta aagtaaaaat ccccaaaaat
     3601 aatacttaag tacagtaatc aagtaaaatt actcaagtac tttacacctc tggttcttga
     3661 ccccctacct tcagcaagcc cagcagatcc actagttcta gagcggccgc caccgcggtg
     3721 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     3781 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3841 aagcataaag tgtaaagcct ggggtgccta atgagtgagc caactcacat taattgcgtt
     3901 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3961 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     4021 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4081 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4141 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4201 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4261 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4321 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4381 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4441 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4501 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4561 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4621 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4681 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4741 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4801 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4861 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4921 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4981 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5041 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5101 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5161 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5221 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5281 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5341 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5401 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5461 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5521 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5581 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5641 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5701 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5761 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccggt acc
//
