LOCUS       sequence-254363-        2041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty Backbone for golden gate cloning compatible with Cermak., et 
            al Toolkit. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_C'0000a
FEATURES             Location/Qualifiers
     source          1..2041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     118..135
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        245..349
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1417..2005
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1906..1925
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
       61 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
      121 gagtcagtga gcgaggaagc ggaagagcgc ccacctgcaa cgccggggcg cgccgatatc
      181 gagctctccc actagcaggt gatgaaaagc cttagggtca ggtggcactt ttcggggaaa
      241 tgtgcgatga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctaat
      301 gagacaataa ccctgataaa tgcttcaatt atattgaaaa aggaagagta tgagtatcca
      361 acatttccgt gtcgccctta ttcccttttt tgcggccttc tgccttcctg tttttgctca
      421 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc gagtgggtta
      481 cattgaactg gatctcaaca gcggcaaaat cttagagagt tttcgccccg aagaacgctt
      541 cccaatgatg agcactttca aagttctgct atgtggcgcg gtgttatccc gtattgatgc
      601 tgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc
      661 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gtagtgctgc
      721 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa
      781 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
      841 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
      901 ggcaacaact ctccgcaaac tattaactgg cgaactactt actcttgctt cacgccagca
      961 actcattgac tggatggagg cggacaaagt tgcaggacca cttctgcgct cggcacttcc
     1021 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     1081 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     1141 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     1201 gcattggtaa ctgtcagacg aagggaaata aatagtagcc cgcctgatgt gcgggctttt
     1261 ttttggtggt ggttaccagt gtcgcttagc taccgtaggt cctcttaggc ggtccgatta
     1321 gcgacatagt catcaatgcc ttgacggcaa aatcccttaa cgtgagtttt cgttccactg
     1381 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1441 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1501 agagctacta actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1561 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1621 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1681 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1741 gggttcgtgc tcacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1801 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1861 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1921 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     1981 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2041 c
//
