LOCUS       sequence-254370-        6961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian, Zebrafish, or Xenopus expression of PhyB-Delta-CreC, one 
            half of the CreLite system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pCS2+PhyB-Delta-CreC
FEATURES             Location/Qualifiers
     source          1..6961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             124..144
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3010..3144
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3034..3053)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3088..3107
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3257..3277)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3257..3275)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3296..3312)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3296..3312)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3309..3331)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3320..3336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3344..3350,3351..3368,3369..3374))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3389..3410
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3527..3544)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3698..4286)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3778..3797)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4457..5248,5249..5317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5080..5099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5318..5422)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5448..5903)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5585..5606)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5797..5816
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5977..6961
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcc tcgaggatat cgccgccacc
      121 atgcccaaga agaagaggaa ggtggtttcc ggagtcgggg gtagtggcgg tggccgtggc
      181 ggtggccgtg gcggagaaga agaaccgtcg tcaagtcaca ctcctaataa ccgaagagga
      241 ggagaacaag ctcaatcgtc gggaacgaaa tctctcagac caagaagcaa cactgaatca
      301 atgagcaaag caattcaaca gtacaccgtc gacgcaagac tccacgccgt tttcgaacaa
      361 tccggcgaat cagggaaatc attcgactac tcacaatcac tcaaaacgac gacgtacggt
      421 tcctctgtac ctgagcaaca gatcacagct tatctctctc gaatccagcg aggtggttac
      481 attcagcctt tcggatgtat gatcgccgtc gatgaatcca gtttccggat catcggttac
      541 agtgaaaacg ccagagaaat gttagggatt atgcctcaat ctgttcctac tcttgagaaa
      601 cctgagattc tagctatggg aactgatgtg agatctttgt tcacttcttc gagctcgatt
      661 ctactcgagc gtgctttcgt tgctcgagag attaccttgt taaatccggt ttggatccat
      721 tccaagaata ctggtaaacc gttttacgcc attcttcata ggattgatgt tggtgttgtt
      781 attgatttag agccagctag aactgaagat cctgcgcttt ctattgctgg tgctgttcaa
      841 tcgcagaaac tcgcggttcg tgcgatttct cagttacagg ctcttcctgg tggagatatt
      901 aagcttttgt gtgacactgt cgtggaaagt gtgagggact tgactggtta tgatcgtgtt
      961 atggtttata agtttcatga agatgagcat ggagaagttg tagctgagag taaacgagat
     1021 gatttagagc cttatattgg actgcattat cctgctactg atattcctca agcgtcaagg
     1081 ttcttgttta agcagaaccg tgtccgaatg atagtagatt gcaatgccac acctgttctt
     1141 gtggtccagg acgataggct aactcagtct atgtgcttgg ttggttctac tcttagggct
     1201 cctcatggtt gtcactctca gtatatggct aacatgggat ctattgcgtc tttagcaatg
     1261 gcggttataa tcaatggaaa tgaagatgat gggagcaatg tagctagtgg aagaagctcg
     1321 atgaggcttt ggggtttggt tgtttgccat cacacttctt ctcgctgcat accgtttccg
     1381 ctaaggtatg cttgtgagtt tttgatgcag gctttcggtt tacagttaaa catggaattg
     1441 cagttagctt tgcaaatgtc agagaaacgc gttttgagaa cgcagacact gttatgtgat
     1501 atgcttctgc gtgactcgcc tgctggaatt gttacacaga gtcccagtat catggactta
     1561 gtgaaatgtg acggtgcagc atttctttac cacgggaagt attacccgtt gggtgttgct
     1621 cctagtgaag ttcagataaa agatgttgtg gagtggttgc ttgcgaatca tgcggattca
     1681 accggattaa gcactgatag tttaggcgat gcggggtatc ccggtgcagc tgcgttaggg
     1741 gatgctgtgt gcggtatggc agttgcatat atcacaaaaa gagactttct tttttggttt
     1801 cgatctcaca ctgcgaaaga aatcaaatgg ggaggcgcta agcatcatcc ggaggataaa
     1861 gatgatgggc aacgaatgca tcctcgttcg tcctttcagg cttttcttga agttgttaag
     1921 agccggagtc agccatggga aactgcggaa atggatgcga ttcactcgct ccagcttatt
     1981 ctgagagact cttttaaaga atctggtggt tctgccggtg gctccggttc tggctccagc
     2041 ggtggcagct ctggtgcgtc cggcacgggt actgcgggtg gcactggcag cggttccggt
     2101 actggctctg gcaaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
     2161 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
     2221 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
     2281 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
     2341 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
     2401 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
     2461 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
     2521 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
     2581 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
     2641 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
     2701 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
     2761 aaggatgact ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc
     2821 gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg
     2881 accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg
     2941 gtgcgcctgc tggaagatgg cgattaggcg gcctcgagcc tctagaacta tagtgagtcg
     3001 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3061 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     3121 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     3181 cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg
     3241 tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     3301 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     3361 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     3421 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     3481 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3541 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3601 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3661 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3721 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3781 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3841 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3901 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3961 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4021 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4081 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     4141 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     4201 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     4261 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     4321 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     4381 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     4441 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     4501 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4561 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4621 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4681 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4741 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4801 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4861 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4921 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4981 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5041 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5101 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     5161 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     5221 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     5281 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     5341 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     5401 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     5461 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     5521 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     5581 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     5641 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     5701 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     5761 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     5821 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     5881 taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg
     5941 gcgatcggtg cgggcctctt cgctattacg ccagtcgacc atagccaatt caatatggcg
     6001 tatatggact catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg
     6061 tatatggact cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg
     6121 gacttggcac catgccaatt caatatggcg gacttggcac tgtgccaact ggggaggggt
     6181 ctacttggca cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat
     6241 tgaattggca tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata
     6301 ggcaatatcc aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat
     6361 tggccctatg ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc
     6421 ccaatgggcg gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc
     6481 ccattgacgt caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct
     6541 attgacgtat atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc
     6601 aatgcccatt gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc
     6661 cattcatatc cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc
     6721 agtacatcaa tatctattaa tagtaacttg gcaagtacat tactattgga aggacgccag
     6781 ggtacattgg cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca
     6841 agtacatccc cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac
     6901 gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa
     6961 c
//
