LOCUS       sequence-254377-       12454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing CreLite, driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12454)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 12454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pLenti-CreLite
FEATURES             Location/Qualifiers
     source          1..12454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        1950..2235
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        2237..2514
                     /label=chicken beta-actin promoter
     intron          2515..2742
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     protein_bind    2747..2771
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2781..2801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5631..5687
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5691..5711
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6285..6309)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        6369..6672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6673..6876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6827..6847
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6904..7602
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             7612..7665
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7666..8265
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7666..7685)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8162..8182
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(8266..8286)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8299..8887
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8352..8372)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8770..8781)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8975..9208
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9280..9401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9317..9336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9371..9390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9441..9576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9596..9615)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9597..9615)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9625..9642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9625..9641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9634..9656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9783..10238
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9870..9889)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10080..10101
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10264..10368
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10369..10437,10438..11229)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10587..10606)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11400..11988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11889..11908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12142..12159
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12276..12297
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12312..12317,12318..12335,12336..12342)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12350..12366
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12355..12377
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12374..12390
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12374..12390
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12409..12429
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12411..12429
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgc gttacataac ttacggtaaa tggcccgcct
     1981 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
     2041 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     2101 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     2161 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     2221 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     2281 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     2341 gcgggggggg ggggggggcg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
     2401 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     2461 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     2521 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     2581 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     2641 tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg ggtattaatg
     2701 tttaattacc tggagcacct gcctgaaatc actttttttc aggttggcaa gtttgtacaa
     2761 aaaagcaggc tgccaccatg cccaagaaga agaggaaggt ggtttccgga gtcgggggta
     2821 gtggcggtgg ccgtggcggt ggccgtggcg gagaagaaga accgtcgtca agtcacactc
     2881 ctaataaccg aagaggagga gaacaagctc aatcgtcggg aacgaaatct ctcagaccaa
     2941 gaagcaacac tgaatcaatg agcaaagcaa ttcaacagta caccgtcgac gcaagactcc
     3001 acgccgtttt cgaacaatcc ggcgaatcag ggaaatcatt cgactactca caatcactca
     3061 aaacgacgac gtacggttcc tctgtacctg agcaacagat cacagcttat ctctctcgaa
     3121 tccagcgagg tggttacatt cagcctttcg gatgtatgat cgccgtcgat gaatccagtt
     3181 tccggatcat cggttacagt gaaaacgcca gagaaatgtt agggattatg cctcaatctg
     3241 ttcctactct tgagaaacct gagattctag ctatgggaac tgatgtgaga tctttgttca
     3301 cttcttcgag ctcgattcta ctcgagcgtg ctttcgttgc tcgagagatt accttgttaa
     3361 atccggtttg gatccattcc aagaatactg gtaaaccgtt ttacgccatt cttcatagga
     3421 ttgatgttgg tgttgttatt gatttagagc cagctagaac tgaagatcct gcgctttcta
     3481 ttgctggtgc tgttcaatcg cagaaactcg cggttcgtgc gatttctcag ttacaggctc
     3541 ttcctggtgg agatattaag cttttgtgtg acactgtcgt ggaaagtgtg agggacttga
     3601 ctggttatga tcgtgttatg gtttataagt ttcatgaaga tgagcatgga gaagttgtag
     3661 ctgagagtaa acgagatgat ttagagcctt atattggact gcattatcct gctactgata
     3721 ttcctcaagc gtcaaggttc ttgtttaagc agaaccgtgt ccgaatgata gtagattgca
     3781 atgccacacc tgttcttgtg gtccaggacg ataggctaac tcagtctatg tgcttggttg
     3841 gttctactct tagggctcct catggttgtc actctcagta tatggctaac atgggatcta
     3901 ttgcgtcttt agcaatggcg gttataatca atggaaatga agatgatggg agcaatgtag
     3961 ctagtggaag aagctcgatg aggctttggg gtttggttgt ttgccatcac acttcttctc
     4021 gctgcatacc gtttccgcta aggtatgctt gtgagttttt gatgcaggct ttcggtttac
     4081 agttaaacat ggaattgcag ttagctttgc aaatgtcaga gaaacgcgtt ttgagaacgc
     4141 agacactgtt atgtgatatg cttctgcgtg actcgcctgc tggaattgtt acacagagtc
     4201 ccagtatcat ggacttagtg aaatgtgacg gtgcagcatt tctttaccac gggaagtatt
     4261 acccgttggg tgttgctcct agtgaagttc agataaaaga tgttgtggag tggttgcttg
     4321 cgaatcatgc ggattcaacc ggattaagca ctgatagttt aggcgatgcg gggtatcccg
     4381 gtgcagctgc gttaggggat gctgtgtgcg gtatggcagt tgcatatatc acaaaaagag
     4441 actttctttt ttggtttcga tctcacactg cgaaagaaat caaatgggga ggcgctaagc
     4501 atcatccgga ggataaagat gatgggcaac gaatgcatcc tcgttcgtcc tttcaggctt
     4561 ttcttgaagt tgttaagagc cggagtcagc catgggaaac tgcggaaatg gatgcgattc
     4621 actcgctcca gcttattctg agagactctt ttaaagaatc tggtggttct gccggtggct
     4681 ccggttctgg ctccagcggt ggcagctctg gtgcgtccgg cacgggtact gcgggtggca
     4741 ctggcagcgg ttccggtact ggctctggca accggaaatg gtttcccgca gaacctgaag
     4801 atgttcgcga ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc
     4861 aacatttggg ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca
     4921 gcaatgctgt ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac
     4981 gtgcaaaaca ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg
     5041 aaaatagcga tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca
     5101 ccctgttacg tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg
     5161 gtgggagaat gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag
     5221 agaaggcact tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg
     5281 tagctgatga tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc
     5341 catctgccac cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc
     5401 gattgattta cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca
     5461 gtgcccgtgt cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca
     5521 tgcaagctgg tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata
     5581 gtgaaacagg ggcaatggtg cgcctgctgg aagatggcga tggaagcgga gctactaact
     5641 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg cccaagaaga
     5701 agaggaaggt gatgttctta ccaaccgatt attgttgcag gttaagcgat caagagtata
     5761 tggagcttgt gtttgagaat ggccagattc ttgcaaaggg ccaaagatcc aacgtttctc
     5821 tgcataatca acgtaccaaa tcgatcatgg atttgtatga ggcagagtat aacgaggatt
     5881 tcatgaagag tatcatccat ggtggtggtg gtgccatcac aaatctcggg gacacgcagg
     5941 ttgttccaca aagtcatgtt gctgctgccc atgaaacaaa catgttggaa agcaataaac
     6001 atgttgacgg tggttctgcc ggtggctccg gttctggctc cagcggtggc agctctggtg
     6061 cgtccggcac gggtactgcg ggtggcactg gcagcggttc cggtactggc tctggcctga
     6121 ctgtgcacca aaacctgcct gccctccctg tggatgccac ctctgatgaa gtcaggaaga
     6181 acctgatgga catgttcagg gacaggcagg ccttctctga acacacctgg aagatgctcc
     6241 tgtctgtgtg cagatcctgg gctgcctggt gcaagctgaa ctgaacccag ctttcttgta
     6301 caaagtggta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     6361 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     6421 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     6481 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     6541 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     6601 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     6661 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     6721 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     6781 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     6841 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatcgcc
     6901 accatgagcg agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg
     6961 gacaaccatc acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag
     7021 accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct
     7081 actagcttcc tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc
     7141 ttcaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg
     7201 ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc
     7261 aagatcagag gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc
     7321 tgggaggcct tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac
     7381 atggccctga agctcgtggg cgggagccat ctgatcgcaa acgccaagac cacatataga
     7441 tccaagaaac ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg
     7501 gaaagaatca aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc
     7561 agatactgcg acctccctag caaactgggg cacaagctta atggaagcgg agagggcagg
     7621 ggaagtcttc taacatgcgg ggacgtggag gaaaatcccg gccccatgac cgagtacaag
     7681 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     7741 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     7801 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     7861 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     7921 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     7981 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     8041 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     8101 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     8161 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     8221 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacaact ttattataca
     8281 tagttgatca attccgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     8341 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     8401 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     8461 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     8521 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     8581 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     8641 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     8701 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     8761 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     8821 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     8881 tccccgcatc gggaattccc gcggttcgct ttaagaccaa tgacttacaa ggcagctgta
     8941 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     9001 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     9061 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9121 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9181 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     9241 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     9301 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9361 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     9421 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     9481 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     9541 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     9601 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     9661 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     9721 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     9781 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     9841 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9901 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9961 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
    10021 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
    10081 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
    10141 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
    10201 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
    10261 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
    10321 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
    10381 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
    10441 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
    10501 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
    10561 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
    10621 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
    10681 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
    10741 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
    10801 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
    10861 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
    10921 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
    10981 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
    11041 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
    11101 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
    11161 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
    11221 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
    11281 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
    11341 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
    11401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
    11461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
    11521 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
    11581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    11641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    11701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    11761 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaagag
    11821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    11881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    11941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    12001 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
    12061 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
    12121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
    12181 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    12241 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    12301 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    12361 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
    12421 cactaaaggg aacaaaagct ggagctgcaa gctt
//
