LOCUS       sequence-25445-e         587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC91
FEATURES             Location/Qualifiers
     source          1..587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..49)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(71..91)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(113..133)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(155..175)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    214..261
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cttctccttt actcattttt tctaccggta ccttacgctt cttctttgga gcagtcattt
       61 tttctgccgg taccttacgc ttcttctttg gagcagtcat tttttctgcc ggtaccttac
      121 gcttcttctt tggagcagtc attttttctg ccggtacctt acgcttcttc tttggagcag
      181 tcattttttc tgagctcggt acccggggat cttgaagttc ctattccgaa gttcctattc
      241 tctagaaagt ataggaactt cagagcgctt ttgaagctag aactagtgga tccacgagag
      301 cgtcggatat tttacctgga aataaattat tttatgatga aagttaaata caataagata
      361 caacgggaaa cgtaagaaaa attcaataaa atcaattgta cacgtaggtt ttcaagcaaa
      421 tttaagaata caattttaca aagtcctaaa tgtcattttt cctgctcaaa cttttgactg
      481 aaatgcataa aaattcgctg ataaaaacaa gcagaatacg ccaaaatagt tgcaaattat
      541 aaaatttaca gaattaatac aaaaaattcg atattttact caaaaaa
//
