LOCUS       sequence-254484-        8048 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, 
            CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Wang X, Friesen E, Muller I, Lemieux M, Dukart R, Maia IB, Kalia S, 
            Schmitt-Ulms G
  TITLE     Rapid Generation of Human Neuronal Cell Models Enabling Inducible 
            Expression of Proteins-of-interest for Functional Studies.
  JOURNAL   Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. 
            eCollection 2020 May 5.
  PUBMED    33659578
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Bio 
            Protoc."; date: "2020-05-5"; volume: "10(9):e3615. doi"; pages: " 
            10.21769/BioProtoc.3615. eCollection 2020 May 5"
COMMENT     Sequence Label: AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP
FEATURES             Location/Qualifiers
     source          1..8048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(990..1023)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1026..1059
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    1064..1089
                     /label=SA
                     /note="splice acceptor site"
     CDS             1113..1166
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1176..1775
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1176..1195)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1672..1692
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    1778..1811
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    1814..2038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2045..2126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2264..2971)
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     primer_bind     complement(3012..3031)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(3093..3115)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3395..3672)
                     /label=chicken beta-actin promoter
     polyA_signal    3939..4060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3950..3969)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(4183..4896,4897..4899,4900..4902))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4186..4896,4897..4899,4900..4902))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4228..4249)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4577..4596
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4836..4857
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4899..4908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6276..6285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6317..6341)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6358..6672)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     primer_bind     complement(6361..6385)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6432..6450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6468..6486
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6503..6521
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6539..6557
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6575..6593
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6610..6628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6646..6664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    complement(6689..6722)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(join(7087..7878,7879..7947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7710..7729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaactgtc atggcatctt ccaggggtcc gagagctcag ctagtcttct tcctccaacc
      841 cgggccccta tgtccacttc aggacagcat gtttgctgcc tccagggatc ctgtgtcccc
      901 gagctgggac caccttatat tcccagggcc ggttaatgtg gctctggttc tgggtacttt
      961 tatctgtccc ctccacccca cagtggggca taacttcgta tagcatacat tatacgaacg
     1021 gtaaagaagt tcctattctc tagaaagtat aggaacttcg cttctgacct cttctcttcc
     1081 tcccacaggg cctcgagaga tctggcagcg gagagggcag aggaagtctt ctaacatgcg
     1141 gtgacgtgga ggagaatccc ggccctaggc tcgagatgac cgagtacaag cccacggtgc
     1201 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     1261 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     1321 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     1381 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     1441 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     1501 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     1561 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     1621 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     1681 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     1741 gcacctggtg catgacccgc aagcccggtg cctgatcgaa gttcctattc tctagaaagt
     1801 ataggaactt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     1861 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     1921 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     1981 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatgggt
     2041 cgaggcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2101 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2161 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     2221 ctgattatga tcctctagag atatcgtcga caagcttatc gatttacccg gggagcatgt
     2281 caaggtcaaa atcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg
     2341 cgtcggtcgg cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt
     2401 ccaggccgaa aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtc
     2461 tcagaagtgg gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat
     2521 gctcctgttc ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca
     2581 gtgcgttctc cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt
     2641 cgtactgttt ctctgttggg cgggtgccga gatgcacttt agccccgtcg cgatgtgaga
     2701 ggagagcaca gcggtatgac ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt
     2761 ccagggggca gaagtgggta tgatgcctgt ccagcatctc gattggcagg gcatcgagca
     2821 gggcccgctt gttcttcacg tgccagtaca gggtaggctg ctcaactccc agcttttgag
     2881 cgagtttcct tgtcgtcagg ccttcgatac cgacaccatt gagtaattcc agagctccgt
     2941 ttatgacttt gctcttgtcc agcctagaca tggtggcact agtccaattc tttgccaaaa
     3001 tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca
     3061 tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc
     3121 ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagc cgctcccccc
     3181 gcacaaaggg ccctcccgga gcccctcaag gctttcacgc agccacagaa aagaaacaag
     3241 ccgtcattaa accaagcgct aattacagcc cggaggagaa gggccgtccc gcccgctcac
     3301 ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg
     3361 gggcacgggg cgaaggcagc gtcgcagcga ctcccgcccg ccgcgcgctt cgctttttat
     3421 agggccgccg ccgccgccgc ctcgccataa aaggaaactt tcggagcgcg ccgctctgat
     3481 tggctgccgc cgcacctctc cgcctcgccc cgccccgccc ctcgccccgc cccgccccgc
     3541 ctggcgcgcg cccccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa
     3601 ataaatacaa aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg
     3661 ggctcacctc gaccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     3721 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     3781 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     3841 gtaaatagtc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     3901 acgtcattat tgacgtcaat ggtacgatct tcctagctta agatacattg atgagtttgg
     3961 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4021 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4081 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4141 caaatgtggt atggctgatt atgatctaga gtcgcggccg ctttacttgt acagctcgtc
     4201 catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt
     4261 ctcgttgggg tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag
     4321 cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc
     4381 gtcctcgatg ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc
     4441 catgatatag acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc
     4501 gtcctccttg aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa
     4561 cttcacctcg gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg
     4621 gacgtagcct tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta
     4681 gcggctgaag cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg
     4741 cagcttgccg gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc
     4801 gccctcgccg gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat
     4861 gggcaccacc ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtggatc
     4921 ccgggcccgc ggtaccgtcg actgcagaat tcttatcaaa ccctgcttgg ccagggaggc
     4981 agacacctcg tcagctagcg tggcgagctg gggcgagtct accatgtcga tgctgccggt
     5041 ggaggagaca ttgctgagat gccgtggaga cgtgtcccca gacaccactg gcgacttgta
     5101 cacgatctcc gccccgtggt ctgtcttggc tttggcgttc tcgcggaagg tcagcttgtg
     5161 ggtttcaatc tttttatttc ctccgccagg gacgtgggtg atattgtcca gggacccaat
     5221 cttcgactgg actctgtcct tgaagtcaag cttctcagat tttacttcca cctggccacc
     5281 tcctggttta tgatggatgt tgcctaatga gccacacttg gaggtcacct tgctcaggtc
     5341 aactggtttg tagactattt gcacactgcc gcctcccagg acgtgtttga tattatcctt
     5401 tgagccacac ttggactgga cgttgctaag atccagcttc ttattaatta tctgcacctt
     5461 cccgcctccc ggctggtgct tcaggttctc agtggagccg atcttggact tgacattctt
     5521 caggtctggc atgggcacgg gggctgtctg caggcggctc ttggcggaag acggcgactt
     5581 gggtggagta cggaccactg ccaccttctt gggctcccgg gtgggtgggg ttggaaggga
     5641 cggggtgcgg gagcggctgc cgggagtgcc tggggagccg gggctgctgt agccgctgcg
     5701 atcccctgat tttggaggtt caccagagct gggtggtgtc tttggagcgg gcggggtttt
     5761 tgctggaatc ctggtggcgt tggcctggcc cttctggcct ggaggggctg ctccccgcgg
     5821 tgtggcgatc ttcgttttac catcagcccc cttggctttt ttgtcatcgc ttccagtccc
     5881 gtctttgctt ttactgacca tgcgagcttg ggtcacgtga ccagcagctt cgtcttccag
     5941 gctgggggtg tctccaatgc ctgcttcttc agctgtggtt ccttctggga tctccgtgtg
     6001 gggctgcgcg gcagcctgct tgccgggagc tccctcatcc actaagggtg ctgtcacatc
     6061 ttccgctgtt ggagtgctct tagcatcaga ggtttcagag cccggttcct cagatccgtc
     6121 ctcagtgggg gtctgcaggg gagattcttt caggccagcg tccgtgtcac cctcttggtc
     6181 ttggtgcatg gtgtagcccc cctgatcttt cctgtccccc aacccgtacg tcccagcgtg
     6241 atcttccatc acttcgaact cctggcgggg ctcagccatg gtggcgctga ggaccggtcc
     6301 aacctgaaaa ccgcggagcc tgcttttttg tacaaacttg ttgatcggaa ttctccaggc
     6361 gatctgacgg ttcactaaac gagctctgct tatataggcc tcccaccgta cacgcctacc
     6421 tcgacatacg ttctctatca ctgataggga gtaaactcga catacgttct ctatcactga
     6481 tagggataaa ctcgacatac gttctctatc actgataggg agtaaactcg acatacgttc
     6541 tctatcactg atagggagta aactcgacat acgttctcta tcactgatag ggagtaaact
     6601 cgacatcgtt ctctatcact gatagggagt aaactcgaca tacgttctct atcactgata
     6661 gggagtaaac tcgacagtac taagctttat aacttcgtat aggatacttt atacgaagtt
     6721 atactaggga caggattggt gacagaaaag ccccatcctt aggcctcctc cttcctagtc
     6781 tcctgatatt gggtctaacc cccacctcct gttaggcaga ttccttatct ggtgacacac
     6841 ccccatttcc tggagccatc tctctccttg ccagaacctc taaggtttgc ttacgatgga
     6901 gccagcatca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6961 ctaaatacat tcaaatatgt atccgctcat gagattatca aaaaggatct tcacctagat
     7021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7201 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     7381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     7441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     7501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7981 tcagggttat tgtctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8041 agaccccg
//
