LOCUS       sequence-25450-e         575 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC220
FEATURES             Location/Qualifiers
     source          1..575
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..46)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(68..88)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(110..130)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(152..172)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    248..295
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    305..329
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctcctttact cattttttct accggtacct tacgcttctt ctttggagca gtcatttttt
       61 ctgccggtac cttacgcttc ttctttggag cagtcatttt ttctgccggt accttacgct
      121 tcttctttgg agcagtcatt ttttctgccg gtaccttacg cttcttcttt ggagcagtca
      181 ttttttctga gctcctgaaa atgttctatg ttatgttagt atcattcgaa acatacccgg
      241 ggatcttgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc
      301 atcaaccact ttgtacaaga aagctgggta ataaaattgt gtaagatgac gctataaaaa
      361 atgacgagct gtgagccaag taggagttga gtaatgaaaa gaaaatgaca gatgtaactg
      421 accctttacc atctaaaaaa ggaatcgtgt gccgtcccaa cagccacctg cttcacacat
      481 gttacccttc tactgacatt gtcgagacaa agtcggtaaa taattggaac atgatgatat
      541 tgctcataag tttatagatg gaaagagatt tcgtt
//
