LOCUS       sequence-254508-       10699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of DDX21 DEV mutation in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10699)
  AUTHORS   Xing YH, Yao RW, Zhang Y, Guo CJ, Jiang S, Xu G, Dong R, Yang L, 
            Chen LL
  TITLE     SLERT Regulates DDX21 Rings Associated with Pol I Transcription.
  JOURNAL   Cell. 2017 May 4;169(4):664-678.e16. doi: 
            10.1016/j.cell.2017.04.011.
  PUBMED    28475895
REFERENCE   2  (bases 1 to 10699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.04"; journalName: "Cell"; date: "2017-05-4- 4";
            volume: "169"; issue: "4"; pages: "664-678.e16"
COMMENT     Sequence Label: p23-DDX21 DEV
FEATURES             Location/Qualifiers
     source          1..10699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3437
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    5799..6366
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5816..6368
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5960..5977)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6187..6206
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6380..7075
                     /codon_start=1
                     /product="Zoanthus green fluorescent protein"
                     /label=ZsGreen1
                     /note="mammalian codon-optimized"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     misc_feature    7089..7677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7142..7162)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7560..7571)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7752..7985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8104..8122)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8190..8294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8295..8363,8364..9155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8513..8532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9326..9914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9815..9834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10068..10085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10325..10346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10361..10366,10367..10384,10385..10391)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10399..10415
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10404..10426
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10423..10439
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10423..10439
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10489..10508
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gaccttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atggactaca
     3421 aagacgatga cgataagccg ggaaaactcc gtagtgacgc tggtttggaa tcagacaccg
     3481 caatgaaaaa aggggagaca ctgcgaaagc aaaccgagga gaaagagaaa aaagagaagc
     3541 caaaatctga taagactgaa gagatagcag aagaggaaga aactgttttc cccaaagcta
     3601 aacaagttaa aaagaaagca gagccttctg aagttgacat gaattctcct aaatccaaaa
     3661 aggcaaaaaa gaaagaggag ccatctcaaa atgacatttc tcctaaaacc aaaagtttga
     3721 gaaagaaaaa ggagcccatt gaaaagaaag tggtttcttc taaaaccaaa aaagtgacaa
     3781 aaaatgagga gccttctgag gaagaaatag atgctcctaa gcccaagaag atgaagaaag
     3841 aaaaggaaat gaatggagaa actagagaga aaagccccaa actgaagaat ggatttcctc
     3901 atcctgaacc ggactgtaac cccagtgaag ctgccagtga agaaagtaac agtgagatag
     3961 agcaggaaat acctgtggaa caaaaagaag gcgctttctc taattttccc atatctgaag
     4021 aaactattaa acttctcaaa ggccgaggag tgaccttcct atttcctata caagcaaaga
     4081 cattccatca tgtttacagc gggaaggact taattgcaca ggcacggaca ggaactggga
     4141 agacattctc ctttgccatc cctttgattg agaaacttca tggggaactg caagacagga
     4201 agagaggccg tgcccctcag gtactggttc ttgcacctac aagagagttg gcaaatcaag
     4261 taagcaaaga cttcagtgac atcacaaaaa agctgtcagt ggcttgtttt tatggtggaa
     4321 ctccctatgg aggtcaattt gaacgcatga ggaatgggat tgatatcctg gttggaacac
     4381 caggtcgtat caaagaccac atacagaatg gcaaactaga tctcaccaaa cttaagcatg
     4441 ttgtcctgca tggagtggac cagatgttgg atatgggatt tgctgatcaa gtggaagaga
     4501 ttttaagtgt ggcatacaag aaagattctg aagacaatcc ccaaacattg cttttttctg
     4561 caacttgccc tcattgggta tttaatgttg ccaagaaata catgaaatct acatatgaac
     4621 aggtggacct gattggtaaa aagactcaga aaacggcaat aactgtggag catctggcta
     4681 ttaagtgcca ctggactcag agggcagcag ttattgggga tgtcatccga gtatatagtg
     4741 gtcatcaagg acgcactatc atcttttgtg aaaccaagaa agaagcccag gagctgtccc
     4801 agaattcagc tataaagcag gatgctcagt ccttgcatgg agacattcca cagaagcaaa
     4861 gggaaatcac cctgaaaggt tttagaaatg gtagttttgg agttttggtg gcaaccaatg
     4921 ttgctgcacg tgggttagac atccctgagg ttgatttggt tatacaaagc tctccaccaa
     4981 aggatgtaga gtcctacatt catcgatccg ggcggacagg cagagctgga aggacggggg
     5041 tgtgcatctg cttttatcag cacaaggaag aatatcagtt agtacaagtg gagcaaaaag
     5101 cgggaattaa gttcaaacga ataggtgttc cttctgcaac agaaataata aaagcttcca
     5161 gcaaagatgc catcaggctt ttggattccg tgcctcccac tgccattagt cacttcaaac
     5221 aatcagctga gaagctgata gaggagaagg gagctgtgga agctctggca gcagcactgg
     5281 cccatatttc aggtgccacg tccgtagacc agcgctcctt gatcaactca aatgtgggtt
     5341 ttgtgaccat gatcttgcag tgctcaattg aaatgccaaa tattagttat gcttggaaag
     5401 aacttaaaga gcagctgggc gaggagattg attccaaagt gaagggaatg gtttttctca
     5461 aaggaaagct gggtgtttgc tttgatgtac ctaccgcatc agtaacagaa atacaggaga
     5521 aatggcatga ttcacgacgc tggcagctct ctgtggccac agagcaacca gaactggaag
     5581 gaccacggga aggatatgga ggcttcaggg gacagcggga aggcagtcga ggcttcaggg
     5641 gacagcggga cggaaacaga agattcagag gacagcggga aggcagtaga ggcccgagag
     5701 gacagcgatc aggaggtggc aacaaaagta acagatccca aaacaaaggc cagaagcgga
     5761 gtttcagtaa agcatttggt caataataat ctagaggatc cctccccccc ccntaacgtt
     5821 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     5881 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     5941 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     6001 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     6061 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     6121 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     6181 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     6241 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     6301 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     6361 tgataatatg gccacacata tggcccagtc caagcacggc ctgaccaagg agatgaccat
     6421 gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg gcgagggcat
     6481 cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg gcggcccctt
     6541 gcccttcgcc gaggacatct tgtccgccgc cttcatgtac ggcaaccgcg tgttcaccga
     6601 gtacccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct acacctggga
     6661 ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca tcaccgtgag
     6721 cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact tccccgccga
     6781 cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga agatcatccc
     6841 cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc tgaaggacgg
     6901 tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc cccgcaagat
     6961 gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg acgccaagaa
     7021 ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc cctgaatcga
     7081 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7141 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7201 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7261 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7321 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7381 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7441 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7501 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7561 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7621 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7681 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7741 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     7801 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7861 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7921 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7981 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     8041 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     8101 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     8161 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8221 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8281 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8341 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8401 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8461 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8521 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8581 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8641 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8701 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8761 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8821 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8881 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8941 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9001 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9061 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9121 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9181 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9241 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9301 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9361 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9421 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9481 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9541 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9601 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9661 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9721 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9781 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9841 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9901 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9961 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10021 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10081 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10141 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    10201 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
    10261 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
    10321 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
    10381 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    10441 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10501 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10561 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10621 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10681 gtactacaaa cttagtagt
//
