LOCUS       sequence-254514-        4808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S::Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_A5801
FEATURES             Location/Qualifiers
     source          1..4808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     127..144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        671..1016
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     959..979
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1034..2686
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1100..1118)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2696..2944
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        3021..3125
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4193..4781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4682..4701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
       61 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      121 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc cacctgctca tgatcggcgc
      181 gccagatttg ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt
      241 acgcagcagg tatcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc
      301 ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag
      361 agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc
      421 ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat
      481 caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg
      541 gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca
      601 tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc
      661 aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt
      721 gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat
      781 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca
      841 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt
      901 caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact
      961 atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg
     1021 ggactcctgc aggatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
     1081 gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
     1141 tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta
     1201 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
     1261 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
     1321 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
     1381 cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat
     1441 tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac
     1501 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
     1561 taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat
     1621 gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg
     1681 cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
     1741 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
     1801 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag
     1861 ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc
     1921 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg
     1981 cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat
     2041 caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     2101 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     2161 ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat
     2221 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     2281 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga
     2341 ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc
     2401 catcttgctc caacacccca acatcttcga cgctggtgtc gcaggtcttc ccgacgatga
     2461 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     2521 agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     2581 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     2641 agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtgacgtc gacgatatga
     2701 agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa gtttgtgttt
     2761 ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat gaatgtaaga
     2821 tcacattata atgaataaac aaatgtttct ataatccatt gtgaatgttt tgttggatct
     2881 cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata tgattaaaga
     2941 taagccagag ctctggtgac ggacgtgggc aggtgtgatg aaaagcctta gggtcaggtg
     3001 gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt atttttctaa atacattcaa
     3061 atatgtatcc gctaatgaga caataaccct gataaatgct tcaattatat tgaaaaagga
     3121 agagtatgag tatccaacat ttccgtgtcg cccttattcc cttttttgcg gccttctgcc
     3181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3241 gtgcccgagt gggttacatt gaactggatc tcaacagcgg caaaatctta gagagttttc
     3301 gccccgaaga acgcttccca atgatgagca ctttcaaagt tctgctatgt ggcgcggtgt
     3361 tatcccgtat tgatgctggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3421 acttggttga gtattcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3481 aattatgtag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3541 cgattggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3661 cgatgcctgt agcaatggca acaactctcc gcaaactatt aactggcgaa ctacttactc
     3721 ttgcttcacg ccagcaactc attgactgga tggaggcgga caaagttgca ggaccacttc
     3781 tgcgctcggc acttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3961 gtgcctcact gattaagcat tggtaactgt cagacgaagg gaaataaata gtagcccgcc
     4021 tgatgtgcgg gctttttttt ggtggtggtt accagtgtcg cttagctacc gtaggtcctc
     4081 ttaggcggtc cgattagcga catagtcatc aatgccttga cggcaaaatc ccttaacgtg
     4141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4261 tttgtttgcc ggatcaagag ctactaactc tttttccgaa ggtaactggc ttcagcagag
     4321 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     4441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4501 ggtcgggctg aacggggggt tcgtgctcac agcccagctt ggagcgaacg acctacaccg
     4561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4801 ttttacgg
//
