LOCUS       sequence-254515-        2577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtU6::sgRNA targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM100
FEATURES             Location/Qualifiers
     source          1..2577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..171
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1239..1827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1728..1747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1981..1998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_RNA        2502..2577
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tttttttccc ggtgacgcag gtgatgaaaa gccttagggt caggtggcac ttttcgggga
       61 aatgtgcgat gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgcta
      121 atgagacaat aaccctgata aatgcttcaa ttatattgaa aaaggaagag tatgagtatc
      181 caacatttcc gtgtcgccct tattcccttt tttgcggcct tctgccttcc tgtttttgct
      241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc ccgagtgggt
      301 tacattgaac tggatctcaa cagcggcaaa atcttagaga gttttcgccc cgaagaacgc
      361 ttcccaatga tgagcacttt caaagttctg ctatgtggcg cggtgttatc ccgtattgat
      421 gctgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
      481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgtagtgct
      541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
      601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      721 atggcaacaa ctctccgcaa actattaact ggcgaactac ttactcttgc ttcacgccag
      781 caactcattg actggatgga ggcggacaaa gttgcaggac cacttctgcg ctcggcactt
      841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc tcgcggtatc
      901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
      961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1021 aagcattggt aactgtcaga cgaagggaaa taaatagtag cccgcctgat gtgcgggctt
     1081 ttttttggtg gtggttacca gtgtcgctta gctaccgtag gtcctcttag gcggtccgat
     1141 tagcgacata gtcatcaatg ccttgacggc aaaatccctt aacgtgagtt ttcgttccac
     1201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1321 caagagctac taactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1381 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1501 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1561 gggggttcgt gctcacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1621 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1861 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1921 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1981 agcgagtcag tgagcgagga agcggaagag cgcccacctg ccaggggacc catggcttcg
     2041 ttgaacaacg gaaactcgac ttgccttccg cacaatacat catttcttct tagctttttt
     2101 tcttcttctt cgttcataca gttttttttt gtttatcagc ttacattttc ttgaaccgta
     2161 gctttcgttt tcttcttttt aactttccat tcggagtttt tgtatcttgt ttcatagttt
     2221 gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa tttgattgaa taaaacatct
     2281 tcattcttaa gatatgaaga taatcttcaa aaggcccctg ggaatctgaa agaagagaag
     2341 caggcccatt tatatgggaa agaacaatag tatttcttat ataggcccat ttaagttgaa
     2401 aacaatcttc aaaagtccca catcgcttag ataagaaaac gaagctgagt ttatatacag
     2461 ctagagtcga agtagtgatt gttggtagta gcgactccat ggttttagag ctagaaatag
     2521 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgc
//
