LOCUS       sequence-254517-        4157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S::IPT::pinII terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM107
FEATURES             Location/Qualifiers
     source          1..4157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1346..1450
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2518..3106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3007..3026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3260..3277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4100..4120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        4109..4155
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 cgggggactc ctgcaggatg gatctgcgtc taattttcgg tccaacttgc acaggaaaga
       61 cgtcgaccgc gatacgtctt gcccagcaga ctggccttcc agtcctttcg ctcgatcggg
      121 tccaatgctg tcctcaactg tcaaccggaa gcggacgacc aacagtggaa gaactgaaag
      181 gaacgacccg tctatacctt gaagatcggc ctctggtgaa gggtatcatc gcagccaagc
      241 aagctcacga aaggctgatc ggggaagtgt acaattatga ggcccacggc gggcttattc
      301 ttgagggagg atctatctcg ttgctcaggt gcatggcgca aagcagttat tggagtaccg
      361 attttcgttg gcatattatt cgccacaagt tagcagacga ggagacattc atgaacgcgg
      421 ccaaggccag agttaggcag atgttgcgcc ctgctgtagg cccatctatt attcaagagt
      481 tggttcatct ttggaatgag cctcggctga ggcccatact gaaagagatc gacggatatc
      541 gatatgccat gttatttgct agccagaacc agatcacacc cgatatgcta ttgcagcttg
      601 acccagatat ggagggtgag ttgattcatg gaatcgctca ggagtatctc atccatgcgc
      661 gccggcagga gcaggaattc cctccagtga gcgtggtcgc tttcgaagga ttcgaaggtc
      721 caccgttcgg aatgtgctag ctcgagccct agacttgtcc atcttctgga ttggccaagt
      781 taattaatgt atgaaataaa aggatgcaca catagtgaca tgctaatcac tataatgtgg
      841 gcatcaaagt tgtgtgttat gtgtaattac taattatctg aataagagaa agagatcatc
      901 catatttctt atcctaaatg aatgtcacgt gtctttataa ttctttgatg aaccagatgc
      961 attttattaa ccaattccat atacatataa atattaatca tatataatta atatcaattg
     1021 ggttagcaaa acaaatctag tctaggtgtg ttttgctaat tattggggga tagtgcaaaa
     1081 agaaatctac gttctcaata attcagatag aaaacttaat aaagtgagat aatttacata
     1141 gattgctttt atcctttgat atatgtgaaa ccatgcatga tataaggaaa atagatagag
     1201 aaataatttt ttacatcgtt gaatatgtaa acaatttaat tcaagaagct aggaatataa
     1261 atattgagga gtttatgatt agagctctcc cactagcagg tgatgaaaag ccttagggtc
     1321 aggtggcact tttcggggaa atgtgcgatg aacccctatt tgtttatttt tctaaataca
     1381 ttcaaatatg tatccgctaa tgagacaata accctgataa atgcttcaat tatattgaaa
     1441 aaggaagagt atgagtatcc aacatttccg tgtcgccctt attccctttt ttgcggcctt
     1501 ctgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     1561 gttgggtgcc cgagtgggtt acattgaact ggatctcaac agcggcaaaa tcttagagag
     1621 ttttcgcccc gaagaacgct tcccaatgat gagcactttc aaagttctgc tatgtggcgc
     1681 ggtgttatcc cgtattgatg ctgggcaaga gcaactcggt cgccgcatac actattctca
     1741 gaatgacttg gttgagtatt caccagtcac agaaaagcat cttacggatg gcatgacagt
     1801 aagagaatta tgtagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     1861 gacaacgatt ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     1921 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     1981 caccacgatg cctgtagcaa tggcaacaac tctccgcaaa ctattaactg gcgaactact
     2041 tactcttgct tcacgccagc aactcattga ctggatggag gcggacaaag ttgcaggacc
     2101 acttctgcgc tcggcacttc cggctggctg gtttattgct gataaatctg gagccggtga
     2161 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     2221 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     2281 gataggtgcc tcactgatta agcattggta actgtcagac gaagggaaat aaatagtagc
     2341 ccgcctgatg tgcgggcttt tttttggtgg tggttaccag tgtcgcttag ctaccgtagg
     2401 tcctcttagg cggtccgatt agcgacatag tcatcaatgc cttgacggca aaatccctta
     2461 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2521 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2581 ggtggtttgt ttgccggatc aagagctact aactcttttt ccgaaggtaa ctggcttcag
     2641 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2701 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2761 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2821 gcagcggtcg ggctgaacgg ggggttcgtg ctcacagccc agcttggagc gaacgaccta
     2881 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2941 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3001 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3061 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3121 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3181 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3241 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccacctgc
     3301 aacgccggcg taatatggcg cgccagattt gccttttcaa tttcagaaag aatgctaacc
     3361 cacagatggt tagagaggct tacgcagcag gtatcatcaa gacgatctac ccgagcaata
     3421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
     3481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
     3541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
     3601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
     3661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
     3721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
     3781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     3841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     3901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     3961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     4021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     4081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     4141 ttcatttgga gagaaca
//
