LOCUS       sequence-254519-        4149 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S::IPT::pinII terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM112
FEATURES             Location/Qualifiers
     source          1..4149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        1692..1796
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2864..3452
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3353..3372
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3606..3623
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactcctgc
      361 aggatggatc tgcgtctaat tttcggtcca acttgcacag gaaagacgtc gaccgcgata
      421 cgtcttgccc agcagactgg ccttccagtc ctttcgctcg atcgggtcca atgctgtcct
      481 caactgtcaa ccggaagcgg acgaccaaca gtggaagaac tgaaaggaac gacccgtcta
      541 taccttgaag atcggcctct ggtgaagggt atcatcgcag ccaagcaagc tcacgaaagg
      601 ctgatcgggg aagtgtacaa ttatgaggcc cacggcgggc ttattcttga gggaggatct
      661 atctcgttgc tcaggtgcat ggcgcaaagc agttattgga gtaccgattt tcgttggcat
      721 attattcgcc acaagttagc agacgaggag acattcatga acgcggccaa ggccagagtt
      781 aggcagatgt tgcgccctgc tgtaggccca tctattattc aagagttggt tcatctttgg
      841 aatgagcctc ggctgaggcc catactgaaa gagatcgacg gatatcgata tgccatgtta
      901 tttgctagcc agaaccagat cacacccgat atgctattgc agcttgaccc agatatggag
      961 ggtgagttga ttcatggaat cgctcaggag tatctcatcc atgcgcgccg gcaggagcag
     1021 gaattccctc cagtgagcgt ggtcgctttc gaaggattcg aaggtccacc gttcggaatg
     1081 tgctagctcg agccctagac ttgtccatct tctggattgg ccaagttaat taatgtatga
     1141 aataaaagga tgcacacata gtgacatgct aatcactata atgtgggcat caaagttgtg
     1201 tgttatgtgt aattactaat tatctgaata agagaaagag atcatccata tttcttatcc
     1261 taaatgaatg tcacgtgtct ttataattct ttgatgaacc agatgcattt tattaaccaa
     1321 ttccatatac atataaatat taatcatata taattaatat caattgggtt agcaaaacaa
     1381 atctagtcta ggtgtgtttt gctaattatt gggggatagt gcaaaaagaa atctacgttc
     1441 tcaataattc agatagaaaa cttaataaag tgagataatt tacatagatt gcttttatcc
     1501 tttgatatat gtgaaaccat gcatgatata aggaaaatag atagagaaat aattttttac
     1561 atcgttgaat atgtaaacaa tttaattcaa gaagctagga atataaatat tgaggagttt
     1621 atgattagag ctcagtgact agcaggtgat gaaaagcctt agggtcaggt ggcacttttc
     1681 ggggaaatgt gcgatgaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1741 cgctaatgag acaataaccc tgataaatgc ttcaattata ttgaaaaagg aagagtatga
     1801 gtatccaaca tttccgtgtc gcccttattc ccttttttgc ggccttctgc cttcctgttt
     1861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcccgag
     1921 tgggttacat tgaactggat ctcaacagcg gcaaaatctt agagagtttt cgccccgaag
     1981 aacgcttccc aatgatgagc actttcaaag ttctgctatg tggcgcggtg ttatcccgta
     2041 ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2101 agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgta
     2161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgattggag
     2221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2341 tagcaatggc aacaactctc cgcaaactat taactggcga actacttact cttgcttcac
     2401 gccagcaact cattgactgg atggaggcgg acaaagttgc aggaccactt ctgcgctcgg
     2461 cacttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2641 tgattaagca ttggtaactg tcagacgaag ggaaataaat agtagcccgc ctgatgtgcg
     2701 ggcttttttt tggtggtggt taccagtgtc gcttagctac cgtaggtcct cttaggcggt
     2761 ccgattagcg acatagtcat caatgccttg acggcaaaat cccttaacgt gagttttcgt
     2821 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2881 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2941 cggatcaaga gctactaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3001 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3061 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3121 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3181 gaacgggggg ttcgtgctca cagcccagct tggagcgaac gacctacacc gaactgagat
     3241 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3301 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3361 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3421 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3481 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3541 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3601 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc acctgcaacg tcccggcgcg
     3661 ccagatttgc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3721 cgcagcaggt atcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3781 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3841 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3901 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     3961 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4021 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4081 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4141 aagggcaat
//
