LOCUS       sequence-25452-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC452
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..41)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(63..83)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(105..125)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(147..167)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    243..290
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    315..335
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     gap             662..672
                     /estimated_length=11
ORIGIN
        1 ttactcnttt tttctaccgg taccttacgc ttcttctttg gagcagtcat tttttctgcc
       61 ggtaccttac gcttcttctt tggagcagtc attttttctg ccggtacctt acgcttcttc
      121 tttggagcag tcattttttc tgccggtacc ttacgcttct tctttggagc agtcattttt
      181 tctgagctcc tgaaaatgtt ctatgttatg ttagtatcat tcgaaacata cccggggatc
      241 ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc agagcggcta
      301 gcccagtgaa ttatcaacta tgtataataa agttgaataa aattgtgtaa gatgacgcta
      361 taaaaaatga cgagttgtga gccaagtagg agttgagtaa tgaaaagaaa atgacagatg
      421 taactgaccc tttaccatct aaaaaaggaa tcgtgtgccg tcccaacagc cacctgcttc
      481 acacatgtta cccttctact gacattgtcg agacaaagtc ggtaaataat tggaacatga
      541 tgatattgct cataagttta tagatggaaa gggatttcgt tgttattttg caagaaaaag
      601 ggaatttgat gtggtttctt anttttnnaa atttgaatnn naannnnaan nannnntncc
      661 cnnnnnnnnn nnannnnnnn nccatgntcc tngntttncg aanngncaan ttttgnttgn
      721 ttgaagacat gcaaacagna catgatcact aacnagagta agttttattn g
//
