LOCUS       sequence-254520-        5440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  35S::AFR5::35S terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pRN104
FEATURES             Location/Qualifiers
     source          1..5440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        650..995
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     938..958
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     polyA_signal    3395..3569
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        3638..3742
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4810..5398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5299..5318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
       61 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
      121 gtgagcgagg aagcggaaga gcgcccacct gcaacgtccc gcagatttgc cttttcaatt
      181 tcagaaagaa tgctaaccca cagatggtta gagaggctta cgcagcaggt atcatcaaga
      241 cgatctaccc gagcaataat ctccaggaaa tcaaatacct tcccaagaag gttaaagatg
      301 cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata tttctcaaga
      361 tcagaagtac tattccagta tggacgattc aaggcttgct tcacaaacca aggcaagtaa
      421 tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg gagtcaaaga
      481 ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc atacagagtc
      541 tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac gacacacttg
      601 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc
      661 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta
      721 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa
      781 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga
      841 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg
      901 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct
      961 ctatataagg aagttcattt catttggaga gaacacgggg gactatgatg gcttcattgt
     1021 cttgtgttga agacaagatg aaaacaagtt gtttggttaa tggtggagga actataacaa
     1081 caacaacatc tcaatctacc ttgcttgaag agatgaagct gttgaaagac cagtcaggta
     1141 caagaaagcc ggtaataaac tcggagctat ggcacgcttg tgcaggccct ttggtgtgtc
     1201 tccctcaagt tgggagctta gtgtattact tctcacaagg tcatagcgag caggttgctg
     1261 tttcaaccag aagatcagca acaacacaag ttcctaatta tccgaacctt ccatctcagt
     1321 tgatgtgtca agtccataat gttactcttc atgctgacaa agacagtgac gaaatctatg
     1381 ctcagatgag tcttcaacct gttcactctg agagagatgt gttccctgta ccagactttg
     1441 gaatgctgag aggaagtaag cacccgactg agtttttctg caaaacactt actgcaagtg
     1501 acacaagcac acatggaggt ttctcagtgc cacgtagagc tgcagagaag ctatttccac
     1561 cattggacta ctcagcacag ccgccaacgc aagagcttgt agttcgagat cttcatgaga
     1621 atacttggac atttcgccat atctaccgag ggcaaccaaa gagacatctc ctaactacag
     1681 gatggagttt gttcgttgga tcgaagagat tgagagctgg ggattctgtt ttgttcatca
     1741 gggatgagaa gtcacaactt atggtcggtg ttaggcgtgc caatcgccaa caaacagcac
     1801 ttccttcatc agttctctca gcggatagta tgcacatcgg tgttcttgct gctgctgctc
     1861 acgcaaccgc caaccgtact ccttttttga tattctataa tccaagagct tgtccagcag
     1921 agttcgtgat ccctctagct aagtaccgta aggcgatatg cgggtctcag ctctcagttg
     1981 gtatgagatt tggaatgatg tttgaaactg aagattccgg gaaacgaagg tacatgggaa
     2041 ctattgttgg aatcagcgat ttggatccgt tgagatggcc tggttctaag tggcgtaacc
     2101 ttcaggtaga atgggatgag cctggatgta atgataaacc tactcgggtc agtccatggg
     2161 atatcgaaac acctgaaagt ctcttcattt ttccttctct gacctcagga ctcaaacgtc
     2221 agctccatcc atcttacttt gctggtgaaa ctgaatgggg tagcttgata aaacggccac
     2281 ttatacgtgt tcctgattcc gcgaatggga ttatgccata tgcatctttc cctagtatgg
     2341 cttcggagca gcttatgaaa atgatgatga ggcctcacaa caaccaaaat gtaccatctt
     2401 tcatgtctga gatgcagcag aatattgtaa tggggaatgg aggtttgcta ggagatatga
     2461 agatgcagca acccctgatg atgaaccaga aatctgagat ggtgcagcca caaaacaagc
     2521 taacagtgaa cccatctgct tctaatacga gtggccaaga acagaatctt tcacagagta
     2581 tgagtgctcc tgctaaacct gagaactcta cactctctgg ttgcagctct ggtagagtcc
     2641 aacatggact tgagcagtca atggaacagg caagccaggt tactacatcc acagtgtgta
     2701 atgaggaaaa ggttaatcag ctacttcaga aaccgggtgc ttcgtcgcct gtacaagctg
     2761 atcaatgtct tgacattact catcagattt accaaccaca gtctgatcca ataaatggat
     2821 tctctttcct ggaaactgat gagctgacat cacaagtctc ttccttccag tctcttgccg
     2881 gatcatacaa gcaaccattc attctatcct cccaggattc ttcagctgtt gtgttaccgg
     2941 attccacaaa ctcaccgctg tttcatgatg tgtgggacac tcagttgaac ggtctcaagt
     3001 ttgaccagtt cagtcccttg atgcagcagg acctttatgc tagtcagaat atctgtatga
     3061 gtaatagcac aaccagtaac attctagatc ctccactctc aaacacagtc cttgatgact
     3121 tctgtgccat caaagacact gatttccaga accacccttc tggttgtttg gttggaaaca
     3181 acaacactag ctttgctcaa gatgtccagt cgcagatcac atcagctagc tttgcagact
     3241 cacaggcctt ctctcgccaa gattttccag ataattctgg aggcactggt acatcttcaa
     3301 gcaatgttga ttttgatgat tgtagtctgc ggcaaaatag taaaggctca tcatggcaga
     3361 aaattgcgac accccgcgtc cgaacctact cgagtttctc cataataatg tgtgagtagt
     3421 tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag catataagaa
     3481 acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc taattcctaa
     3541 aaccaaaatc cagtactaaa atccagatcc cccgaattaa gtgactagca ggtgatgaaa
     3601 agccttaggg tcaggtggca cttttcgggg aaatgtgcga tgaaccccta tttgtttatt
     3661 tttctaaata cattcaaata tgtatccgct aatgagacaa taaccctgat aaatgcttca
     3721 attatattga aaaaggaaga gtatgagtat ccaacatttc cgtgtcgccc ttattccctt
     3781 ttttgcggcc ttctgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3841 tgctgaagat cagttgggtg cccgagtggg ttacattgaa ctggatctca acagcggcaa
     3901 aatcttagag agttttcgcc ccgaagaacg cttcccaatg atgagcactt tcaaagttct
     3961 gctatgtggc gcggtgttat cccgtattga tgctgggcaa gagcaactcg gtcgccgcat
     4021 acactattct cagaatgact tggttgagta ttcaccagtc acagaaaagc atcttacgga
     4081 tggcatgaca gtaagagaat tatgtagtgc tgccataacc atgagtgata acactgcggc
     4141 caacttactt ctgacaacga ttggaggacc gaaggagcta accgcttttt tgcacaacat
     4201 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4261 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca actctccgca aactattaac
     4321 tggcgaacta cttactcttg cttcacgcca gcaactcatt gactggatgg aggcggacaa
     4381 agttgcagga ccacttctgc gctcggcact tccggctggc tggtttattg ctgataaatc
     4441 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4501 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4561 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag acgaagggaa
     4621 ataaatagta gcccgcctga tgtgcgggct tttttttggt ggtggttacc agtgtcgctt
     4681 agctaccgta ggtcctctta ggcggtccga ttagcgacat agtcatcaat gccttgacgg
     4741 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4801 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4861 accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt ttccgaaggt
     4921 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4981 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5041 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5101 accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc ccagcttgga
     5161 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5221 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5281 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5341 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5401 cgccagcaac gcggcctttt tacggttcct ggccttttgc
//
