LOCUS       sequence-254522-        6832 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module D with ZmUbi1::BBM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6832)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 6832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_D4910
FEATURES             Location/Qualifiers
     source          1..6832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             943..954
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        2739..2843
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3911..4499
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4400..4419
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4653..4670
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 atggccactg tgaacaactg gctcgctttc tccctctccc cgcaggagct gccgccctcc
       61 cagacgacgg actccacact catctcggcc gccaccgccg accatgtctc cggcgatgtc
      121 tgcttcaaca tcccccaaga ttggagcatg aggggatcag agctttcggc gctcgtcgcg
      181 gagccgaagc tggaggactt cctcggcggc atctccttct ccgagcagca tcacaaggcc
      241 aactgcaaca tgatacccag cactagcagc acagtttgct acgccagctc aggtgctagc
      301 accggctacc atcaccagct gtaccaccag cccaccagct cagcgctcca cttcgcggac
      361 tccgtaatgg tggcctcctc ggccggtgtc cacgacggcg gtgccatgct cagcgcggcc
      421 gccgctaacg gtgtcgctgg cgctgccagt gccaacggcg gcggcatcgg gctgtccatg
      481 attaagaact ggctgcggag ccaaccggcg cccatgcagc cgagggtggc ggcggctgag
      541 ggcgcgcagg ggctctcttt gtccatgaac atggcgggga cgacccaagg cgctgctggc
      601 atgccacttc tcgctggaga gcgcgcacgg gcgcccgaga gtgtatccac gtcagcacag
      661 ggtggagccg tcgtcgtcac ggcgccgaag gaggatagcg gtggcagcgg tgttgccggc
      721 gctctagtag ccgtgagcac ggacacgggt ggcagcggcg gcgcgtcggc tgacaacacg
      781 gcaaggaaga cggtggacac gttcgggcag cgcacgtcga tttaccgtgg cgtgacaagg
      841 catagatgga ctgggagata tgaggcacat ctttgggata acagttgcag aagggaaggg
      901 caaactcgta agggtcgtca agtctattta ggtggctatg ataaagagga gaaagctgct
      961 agggcttatg atcttgctgc tctgaagtac tggggtgcca caacaacaac aaattttcca
     1021 gtgagtaact acgaaaagga gctggaggac atgaagcaca tgacaaggca ggagtttgta
     1081 gcgcctctga gaaggaagtc cagtggtttc tccagaggtg catccattta caggggagtg
     1141 actaggcatc accaacatgg aagatggcaa gcacggattg gacgagttgc agggaacaag
     1201 gatctttact tgggcacctt cagcacccag gaggaggcag cggaggcgta cgacatcgcg
     1261 gcgatcaagt tccgcggcct caacgccgtc accaacttcg acatgagccg ctacgacgtg
     1321 aagtccatcc tggacagcag cgccctcccc atcggcagcg ccgccaagcg cctcaaggag
     1381 gccgaggccg cagcgtccgc gcagcaccac catgcgggtg tcgtttccta tgacgttggg
     1441 aggattgcca gccaactggg agatggcggt gccctcgctg cggcctatgg tgctcactat
     1501 cacggtgccg cgtggccaac gattgcattc cagccgggcg cggcgtccac cggactgtac
     1561 catccttacg cgcagcagcc tatgcgcggc ggtggatggt gtaaacaaga gcaagatcac
     1621 gctgtgatag cagcggcaca ctccttgcag gatcttcatc atttgaatct cggagccgcc
     1681 ggggcccacg actttttctc ggcagggcag caggccgccg ccgctgcgat gcacggcctg
     1741 ggtagcatcg acagtgcgtc gctggagcac agcaccggct ccaactccgt cgtctacaac
     1801 ggcggggtcg gcgacagcaa cggcgccagc gccgtcggcg gcagtggcgg tggctacatg
     1861 atgccgatga gcgctgccgg agcaaccact acatcggcaa tggtgagcca cgagcaggtc
     1921 catgcacggg cctacgacga agccaagcag gctgctcaga tggggtacga gagctacctg
     1981 gtgaacgcgg agaacaatgg tggcggaagg atgtctgcat gggggactgt cgtgtctgca
     2041 gccgcggcgg cagcagcaag cagcaacgac aacatggccg ccgacgtggg ccacggcggc
     2101 gcgcagctgt tcagtgtctg gaacgacact taactcgagc cctagacttg tccatcttct
     2161 ggattggcca agttaattaa tgtatgaaat aaaaggatgc acacatagtg acatgctaat
     2221 cactataatg tgggcatcaa agttgtgtgt tatgtgtaat tactaattat ctgaataaga
     2281 gaaagagatc atccatattt cttatcctaa atgaatgtca cgtgtcttta taattctttg
     2341 atgaaccaga tgcattttat taaccaattc catatacata taaatattaa tcatatataa
     2401 ttaatatcaa ttgggttagc aaaacaaatc tagtctaggt gtgttttgct aattattggg
     2461 ggatagtgca aaaagaaatc tacgttctca ataattcaga tagaaaactt aataaagtga
     2521 gataatttac atagattgct tttatccttt gatatatgtg aaaccatgca tgatataagg
     2581 aaaatagata gagaaataat tttttacatc gttgaatatg taaacaattt aattcaagaa
     2641 gctaggaata taaatattga ggagtttatg attagagctc agtgactagc aggtgatgaa
     2701 aagccttagg gtcaggtggc acttttcggg gaaatgtgcg atgaacccct atttgtttat
     2761 ttttctaaat acattcaaat atgtatccgc taatgagaca ataaccctga taaatgcttc
     2821 aattatattg aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc cttattccct
     2881 tttttgcggc cttctgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2941 atgctgaaga tcagttgggt gcccgagtgg gttacattga actggatctc aacagcggca
     3001 aaatcttaga gagttttcgc cccgaagaac gcttcccaat gatgagcact ttcaaagttc
     3061 tgctatgtgg cgcggtgtta tcccgtattg atgctgggca agagcaactc ggtcgccgca
     3121 tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg
     3181 atggcatgac agtaagagaa ttatgtagtg ctgccataac catgagtgat aacactgcgg
     3241 ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca
     3301 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     3361 acgacgagcg tgacaccacg atgcctgtag caatggcaac aactctccgc aaactattaa
     3421 ctggcgaact acttactctt gcttcacgcc agcaactcat tgactggatg gaggcggaca
     3481 aagttgcagg accacttctg cgctcggcac ttccggctgg ctggtttatt gctgataaat
     3541 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3601 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3661 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gacgaaggga
     3721 aataaatagt agcccgcctg atgtgcgggc ttttttttgg tggtggttac cagtgtcgct
     3781 tagctaccgt aggtcctctt aggcggtccg attagcgaca tagtcatcaa tgccttgacg
     3841 gcaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     3901 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3961 caccgctacc agcggtggtt tgtttgccgg atcaagagct actaactctt tttccgaagg
     4021 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     4081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgctcacag cccagcttgg
     4261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4681 agcgcccacc tgcaacgtcc cggcgcgcct gcagtgcagc gtgacccggt cgtgcccctc
     4741 tctagagata atgagcattg catgtctaag ttataaaaaa ttaccacata ttttttttgt
     4801 cacacttgtt tgaagtgcag tttatctatc tttatacata tatttaaact ttactctacg
     4861 aataatataa tctatagtac tacaataata tcagtgtttt agagaatcat ataaatgaac
     4921 agttagacat ggtctaaagg acaattgagt attttgacaa caggactcta cagttttatc
     4981 tttttagtgt gcatgtgttc tccttttttt ttgcaaatag cttcacctat ataatacttc
     5041 atccatttta ttagtacatc catttagggt ttagggttaa tggtttttat agactaattt
     5101 ttttagtaca tctattttat tctattttag cctctaaatt aagaaaacta aaactctatt
     5161 ttagtttttt tatttaataa tttagatata aaatagaata aaataaagtg actaaaaatt
     5221 aaacaaatac cctttaagaa attaaaaaaa ctaaggaaac atttttcttg tttcgagtag
     5281 ataatgccag cctgttaaac gccgtcgacg agtctaacgg acaccaacca gcgaaccagc
     5341 agcgtcgcgt cgggccaagc gaagcagacg gcacggcatc tctgtcgctg cctctggacc
     5401 cctctcgaga gttccgctcc accgttggac ttgctccgct gtcggcatcc agaaattgcg
     5461 tggcggagcg gcagacgtga gccggcacgg caggcggcct cctcctcctc tcacggcacc
     5521 ggcagctacg ggggattcct ttcccaccgc tccttcgctt tcccttcctc gcccgccgta
     5581 ataaatagac accccctcca caccctcttt ccccaacctc gtgttgttcg gagcgcacac
     5641 acacacaacc agatctcccc caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc
     5701 tcgtcctccc cccccccctc tctaccttct ctagatcggc gttccggtcc atggttaggg
     5761 cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc
     5821 tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc
     5881 cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt
     5941 tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata
     6001 tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat
     6061 gatgtggtct ggttgggcgg tcgttctaga tcggagtaga attaattctg tttcaaacta
     6121 cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga
     6181 attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt
     6241 actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg
     6301 gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt
     6361 attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga
     6421 tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca
     6481 tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata
     6541 aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca
     6601 gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt
     6661 cttttgtcga tgctcaccct gttgtttggt gttacttcct gcaggcttcc ctaacctttg
     6721 cactgtccaa aatggcttcc tgatcccctc acttcctcga atcaatctaa gaagaaactc
     6781 aagccgcaac cattaggggc agattaattg ctgcactttc agataatcaa gc
//
