LOCUS       sequence-254523-       13601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13601)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 13601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM131
FEATURES             Location/Qualifiers
     source          1..13601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        966..1311
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1254..1274
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1329..2981
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1395..1413)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2991..3239
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        3732..3807
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    5466..5640
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7322..7346
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8647..9276
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9705..10778
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10844..11038
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11274..11296)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11382..11522
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11437..11456
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11537..11554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11708..12296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11788..11807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12383..13177)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgccag
      481 atttgccttt tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca
      541 gcaggtatca tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc
      601 aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa
      661 gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac
      721 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag
      781 gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa
      841 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg
      901 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
      961 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
     1021 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
     1081 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
     1141 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     1201 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     1261 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact
     1321 cctgcaggat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
     1381 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     1441 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
     1501 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     1561 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     1621 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     1681 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
     1741 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
     1801 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     1861 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
     1921 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
     1981 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     2041 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     2101 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
     2161 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     2221 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     2281 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     2341 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     2401 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     2461 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     2521 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     2581 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     2641 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     2701 tgctccaaca ccccaacatc ttcgacgctg gtgtcgcagg tcttcccgac gatgacgccg
     2761 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     2821 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     2881 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     2941 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgtg acgtcgacga tatgaagatg
     3001 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
     3061 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatcaca
     3121 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
     3181 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataagc
     3241 cagagctctg gtgacggacc catggcttcg ttgaacaacg gaaactcgac ttgccttccg
     3301 cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca gttttttttt
     3361 gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat
     3421 tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa
     3481 ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa
     3541 aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag
     3601 tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag
     3661 ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt gttggtagta
     3721 gcgactccat ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3781 cttgaaaaag tggcaccgag tcggtgcttt ttttcccggg gcgcgccgat atcgagctct
     3841 cccgctggca gacatactgt cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga
     3901 aataatgcgt ctgacaaagg ttaggtcggc tgcctttaat caataccaaa gtggtcccta
     3961 ccacgatgga aaaactgtgc agtcggtttg gctttttctg acgaacaaat aagattcgtg
     4021 gccgacaggt gggggtccac catgtgaagg catcttcaga ctccaataat ggagcaatga
     4081 cgtaagggct tacgaaataa gtaagggtag tttgggaaat gtccactcac ccgtcagtct
     4141 ataaatactt agcccctccc tcattgttaa gggagcaaaa tctcagagag atagtcctag
     4201 agagagaaag agagcaagta gcctagaagt agtcaaggcg gcgaagtatt caggcacgtg
     4261 gccaggaaga agaaaagcca agacgacgaa aacaggtaag agctaagctt atggagagtg
     4321 gttccaacag cacttcttgt ccaatggctt ttgccgggga taatagtgat ggtccgatgt
     4381 gtcctatgat gatgatgatg ccgcccatca tgacatcaca tcaacatcat ggtcatgatc
     4441 atcaacatca acaacaagaa catgatggtt atgcatatca gtcacaccac caacaaagta
     4501 gttccctttt tcttcaatca ctagctcctc cccaaggaac taagaacaaa gttgcttctt
     4561 cttcttctcc ttcctcttgt gctcctgcct attctctaat ggagatccat cataacgaaa
     4621 tcgttgcagg aggaatcaac ccttgctcct cttcctcttc ttcagcctct gtcaaggcca
     4681 agatcatggc tcatcctcac taccaccgcc tcttggccgc ttatgtcaat tgtcagaagg
     4741 ttggagcacc accggaggtt gtggcgaggc tagaggaggc atgctcgtct gccgcagccg
     4801 ctgccgcatc tatgggacca acaggatgtc taggtgaaga tccagggctt gatcaattca
     4861 tggaagctta ctgtgaaatg ctcgttaagt atgagcaaga gctctccaaa cctttcaagg
     4921 aagctatggt cttccttcaa cgtgtcgagt gtcaattcaa atccctctct ctatcctcac
     4981 cttcctcttt ctccggttat ggagagacag caattgatag gaacaataat gggtcatccg
     5041 aggaagaagt cgatatgaac aatgaatttg tagatccaca agctgaggat agagagctta
     5101 aaggacagct cttgcgcaag tacagtggtt acttagggag cctcaagcaa gagttcatga
     5161 agaagaggaa gaaaggaaag ctccctaaag aagctcgtca acaactgctt gattggtgga
     5221 gccgtcacta caaatggcct tacccttcgg agcaacaaaa gctcgccctt gcggaatcaa
     5281 cggggctgga ccagaaacag ataaacaatt ggttcataaa ccagaggaaa cggcattgga
     5341 agccgtcgga ggacatgcag tttgtagtaa tggacgcaac acatcctcac cattacttca
     5401 tggataatgt cttgggcaat cctttcccaa tggatcacat ctcctccacc atgctttgac
     5461 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
     5521 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
     5581 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
     5641 ccccgaatta agtgtttgat cgccggcggt accgagtgta cttcaagtca gtgggaaatc
     5701 aataaaatga ttattttatg aatatatttc attgtgcaag tagatagaaa ttacatatgt
     5761 tacataacac acgaaataaa caaaaaaaga caatccaaaa acaaacaccc caaaaaaaat
     5821 aatcacttta gataaactcg tatgaggaga ggcacgttca gtgactcgac gattcccgag
     5881 caaaaaaagt ctccccgtca cacatgtagt gggtgacgca attatcttta aagtaatcct
     5941 tctgttgact tgtcattgat aacatccagt cttcgtcagg attgcaaaga attatagaag
     6001 ggatcccacc ttttattttc ttcttttttc catatttagg gttgacagtg aaatcagact
     6061 ggcaacctat taattgcttc cacaatggga cgaacttgaa ggggatgtcg tcgatgatat
     6121 tataggtggc gtgttcatcg tagttggtga aatcgatggt accgttccaa tagttgtgtc
     6181 gtccgagact tctagcccag gtggtctttc cggtacgagt tggtccgcag atgtagaggc
     6241 tggggtgtcg gattccattc cttccattgt ccttgttaaa tcggccatcc attcaaggtc
     6301 agattgagct tgttggtatg agacaggatg tatgtaagta taagcgtcta tgcttacatg
     6361 gtatagatgg gtttccctcc aggagtgtag atcttcgtgg cagcgaagat ctgattctgt
     6421 gaagggcgac acatacggtt caggttgtgg agggaataat ttgttggctg aatattccag
     6481 ccattgaagc tttgttgccc attcatgagg gaattcttcc ttgatcatgt caagatattc
     6541 ctccttagac gttgcagtct ggataatagt tctccatcgt gcgtcagatt tgcgaggaga
     6601 aaccttatga tctcggaaat ctcctctggt tttaatatct ccgtcctttg atatgtaatc
     6661 aaggacttgt ttagagtttc tagctggctg gatattaggg tgatttcctt caaaatcgaa
     6721 aaaagaagga tccctaatac aaggtttttt atcaagctgg agaagagcat gatagtgggt
     6781 agtgccatct tgatgaagct cagaagcaac accaaggaag aaaataagaa aaggtgtgag
     6841 tttctcccag agaaactgga ataaatcatc tctttgagat gagcacttgg gataggtaag
     6901 gaaaacatat ttagattgga gtctgaagtt cttactagca gaaggcatgt tgttgtgact
     6961 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
     7021 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
     7081 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
     7141 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
     7201 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
     7261 caggtatttc cgttggatac gaattattcg tacgaccctc atagtttaaa ctatcagtgt
     7321 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
     7381 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
     7441 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
     7501 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     7561 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     7621 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     7681 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     7741 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     7801 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     7861 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     7921 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     7981 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     8041 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     8101 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     8161 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     8221 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     8281 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     8341 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     8401 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacggct caaccgtgcg
     8461 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     8521 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     8581 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     8641 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     8701 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     8761 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     8821 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     8881 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     8941 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     9001 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     9061 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     9121 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     9181 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     9241 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     9301 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     9361 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     9421 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     9481 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     9541 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     9601 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc
     9661 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     9721 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     9781 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     9841 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     9901 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     9961 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
    10021 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
    10081 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
    10141 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
    10201 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
    10261 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
    10321 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
    10381 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
    10441 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
    10501 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
    10561 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
    10621 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
    10681 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
    10741 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
    10801 aaattgccct agcaggggaa aaaggtcgaa aaggcctctt tcctgtggat agcacgtaca
    10861 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
    10921 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
    10981 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
    11041 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
    11101 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
    11161 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
    11221 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
    11281 cacatgcagc tcccggaaac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
    11341 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
    11401 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
    11461 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
    11521 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11581 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11701 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11761 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    11821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    11881 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    12001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    12061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    12121 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    12181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    12241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    12301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    12361 ttttggtcat gcattctagg tactaaaaca attcatccag taaaatataa tattttattt
    12421 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc
    12481 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc
    12541 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt
    12601 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat
    12661 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat
    12721 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg
    12781 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct
    12841 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg
    12901 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa
    12961 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg
    13021 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct
    13081 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca
    13141 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct
    13201 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt
    13261 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt
    13321 ggcgtataac atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac
    13381 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
    13441 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
    13501 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
    13561 tttgtgccga gctgccggtc ggggagctgt tggctggctg g
//
