LOCUS       sequence-254524-        2041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty Backbone for golden gate cloning compatible with Cermak., et 
            al Toolkit. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMOD_D0000a
FEATURES             Location/Qualifiers
     source          1..2041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        116..220
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1288..1876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1777..1796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2030..2041,1..6)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgaggaag cggaagagcg cccacctgca acgtcccggc gcgccgatat cgagctcagt
       61 gactagcagg tgatgaaaag ccttagggtc aggtggcact tttcggggaa atgtgcgatg
      121 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctaa tgagacaata
      181 accctgataa atgcttcaat tatattgaaa aaggaagagt atgagtatcc aacatttccg
      241 tgtcgccctt attccctttt ttgcggcctt ctgccttcct gtttttgctc acccagaaac
      301 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc cgagtgggtt acattgaact
      361 ggatctcaac agcggcaaaa tcttagagag ttttcgcccc gaagaacgct tcccaatgat
      421 gagcactttc aaagttctgc tatgtggcgc ggtgttatcc cgtattgatg ctgggcaaga
      481 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt caccagtcac
      541 agaaaagcat cttacggatg gcatgacagt aagagaatta tgtagtgctg ccataaccat
      601 gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga aggagctaac
      661 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      721 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      781 tctccgcaaa ctattaactg gcgaactact tactcttgct tcacgccagc aactcattga
      841 ctggatggag gcggacaaag ttgcaggacc acttctgcgc tcggcacttc cggctggctg
      901 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      961 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     1021 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1081 actgtcagac gaagggaaat aaatagtagc ccgcctgatg tgcgggcttt tttttggtgg
     1141 tggttaccag tgtcgcttag ctaccgtagg tcctcttagg cggtccgatt agcgacatag
     1201 tcatcaatgc cttgacggca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctact
     1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1621 ctcacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2041 g
//
