LOCUS       sequence-25453-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC93
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..47)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(69..89)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(111..131)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(153..173)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    212..259
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    298..422
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(565..584)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tctnctttac tcattttttc taccggtacc ttacgcttct tctttggagc agtcattttt
       61 tctgccggta ccttacgctt cttctttgga gcagtcattt tttctgccgg taccttacgc
      121 ttcttctttg gagcagtcat tttttctgcc ggtaccttac gcttcttctt tggagcagtc
      181 attttttctg agctcggtac ccggggatct tgaagttcct attccgaagt tcctattctc
      241 tagaaagtat aggaacttca gagcgctttt gaagctagaa ctagtggatc tgatatcacc
      301 actttgtaca agaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
      361 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      421 tggtcgacct gcagactggc tgtgtataag ggagcctgac atttatattc cccagaacat
      481 caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca cttcttcccc
      541 gataacggag accggcacac tggccatatc ggtggtcatc atgcgccagc tttcatcccc
      601 gatatgcacc accgggtaaa gtt
//
