LOCUS       sequence-254534-       12918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive GFP expression, WUS2, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 12918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM119
FEATURES             Location/Qualifiers
     source          1..12918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    71..95
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        1036..1381
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1324..1344
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1432..1460)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2055..2075
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      2126..2374
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        2864..2939
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3042..3221
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3074..3257
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6709..6733
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8034..8663
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9092..10165
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10231..10425
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10661..10683)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10769..10909
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10824..10843
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10924..10941)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11095..11683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11175..11194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11770..12564)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
       61 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
      121 acacattgcg gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt
      181 tgttgtgact ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag
      241 gcaggggtat tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga
      301 cgaagtcttt gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc
      361 cttatcgcga gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac
      421 tagggttaaa ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg
      481 tatattgtat caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc
      541 ggcgcgccag atttgccttt tcaatttcag aaagaatgct aacccacaga tggttagaga
      601 ggcttacgca gcaggtatca tcaagacgat ctacccgagc aataatctcc aggaaatcaa
      661 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
      721 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
      781 cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac
      841 tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa
      901 gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt
      961 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga
     1021 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
     1081 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
     1141 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
     1201 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     1261 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     1321 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac
     1381 acgggggact cctgcaggat ggtgagtaaa ggagaagaac ttttcactgg agttgtccca
     1441 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     1501 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     1561 cctgttccat ggccaaccct ggtcaccacc ctgacctacg gcgtgcagtg cttctcccgt
     1621 taccctgatc atatgaagcg gcacgacttc ttcaagagcg ccatgcctga gggatacgtg
     1681 caggagagga ccatcttctt caaggacgac gggaactaca agacacgtgc tgaagtcaag
     1741 tttgagggag acaccctcgt caacaggatc gagcttaagg gaatcgattt caaggaggac
     1801 ggaaacatcc tcggccacaa gttggaatac aactacaact cccacaacgt atacatcatg
     1861 gccgacaagc aaaagaacgg catcaaagcc aacttcaaga cccgccacaa catcgaagac
     1921 ggcggcgtgc aactcgctga tcattatcaa caaaatactc caattggcga tggccctgtc
     1981 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
     2041 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
     2101 gatgaactat acaaataact cgagatatga agatgaagat gaaatatttg gtgtgtcaaa
     2161 taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt
     2221 ggctttttct aatattaaat gaatgtaaga tcacattata atgaataaac aaatgtttct
     2281 ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct
     2341 atggtatgga ctatggaata tgattaaaga taaggagctc cggtgacgga cccatggctt
     2401 cgttgaacaa cggaaactcg acttgccttc cgcacaatac atcatttctt cttagctttt
     2461 tttcttcttc ttcgttcata cagttttttt ttgtttatca gcttacattt tcttgaaccg
     2521 tagctttcgt tttcttcttt ttaactttcc attcggagtt tttgtatctt gtttcatagt
     2581 ttgtcccagg attagaatga ttaggcatcg aaccttcaag aatttgattg aataaaacat
     2641 cttcattctt aagatatgaa gataatcttc aaaaggcccc tgggaatctg aaagaagaga
     2701 agcaggccca tttatatggg aaagaacaat agtatttctt atataggccc atttaagttg
     2761 aaaacaatct tcaaaagtcc cacatcgctt agataagaaa acgaagctga gtttatatac
     2821 agctagagtc gaagtagtga ttgttggtag tagcgactcc atggttttag agctagaaat
     2881 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     2941 ttttttcccg ggcgcgccga tcatgagcgg agaattaagg gagtcacgtt atgacccccg
     3001 ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga
     3061 gccactcagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca gaaaccatta
     3121 ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct tgtcaaaaat
     3181 gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat attcactctc
     3241 aatccaaata atctgcaccg tacctgcagg gtccgagcta ggtcacagaa gcgctcagga
     3301 aggccgctga gatagaggca tggcggccaa tgcgggcggc ggtggagcgg gaggaggcag
     3361 cggcagcggc agcgtggctg cgccggcggt gtgccgcccc agcggctcgc ggtggacgcc
     3421 gacgccggag cagatcagga tgctgaagga gctgtactac ggctgcggca tccggtcgcc
     3481 cagctcggag cagatccagc gcatcaccgc catgctgcgg cagcacggca agatcgaggg
     3541 caagaacgtc ttctactggt tccagaacca caaggcccgc gagcgccaga agcgccgcct
     3601 caccagcctc gacgtgaacg tgcccgccgc cggcgcggcc gacgccacca ccagccaact
     3661 cggcgtcctc tcgctgtcgt cgccgccgcc ttcaggcgcg gcgcctccct cgcccaccct
     3721 cggcttctac gccgccggca atggcggcgg atcggctgtg ctgctggaca cgagttccga
     3781 ctggggcagc agcggcgctg cgatggccac cgagacatgc ttcctccagg actacatggg
     3841 cgtgacggac acgggcagct cgtcgcagtg gccacgcttc tcgtcgtcgg acacgataat
     3901 ggcggcggcc gcggcgcggg cggcgacgac gcgggcgccc gagactctcc ctctcttccc
     3961 gacctgcggc gacgacggcg gcagcggtag cagcagctac ttgccgttct ggggtgccgc
     4021 gtccacaact gccggcgcca cttcttccgt tgcgatccag cagcaacacc agctgcagga
     4081 gcagtacagc ttttacagca acagcaacag cacccagctg gccggcaccg gcaaccaaga
     4141 cgtatcggca acagcagcag cagccgccgc cctggagctg agcctcagct catggtgctc
     4201 cccttaccct gctgcaggga gtatgtgaga gcaacgcgag ctgccactgc tcttcactta
     4261 tgtctctgga atggaaggag gaggaagtga gcatagcgtt ggtgcgttgc tgtcattgtc
     4321 ctaggttagt agctagtgcc agttactagt aagcatcagg cataggagta tgtagtagaa
     4381 gcatgcacgt tgccggccag ccaggcttta gacgggaaaa gaatttggtg cagccggctg
     4441 caaaacagga tgtttacagc ccccccctcg agccctagac ttgtccatct tctggattgg
     4501 ccaagttaat taatgtatga aataaaagga tgcacacata gtgacatgct aatcactata
     4561 atgtgggcat caaagttgtg tgttatgtgt aattactaat tatctgaata agagaaagag
     4621 atcatccata tttcttatcc taaatgaatg tcacgtgtct ttataattct ttgatgaacc
     4681 agatgcattt tattaaccaa ttccatatac atataaatat taatcatata taattaatat
     4741 caattgggtt agcaaaacaa atctagtcta ggtgtgtttt gctaattatt gggggatagt
     4801 gcaaaaagaa atctacgttc tcaataattc agatagaaaa cttaataaag tgagataatt
     4861 tacatagatt gcttttatcc tttgatatat gtgaaaccat gcatgatata aggaaaatag
     4921 atagagaaat aattttttac atcgttgaat atgtaaacaa tttaattcaa gaagctagga
     4981 atataaatat tgaggagttt atgattagag ctctcccggc gcgccgatat cgagctcagt
     5041 gtttgatcgc cggcggtacc gagtgtactt caagtcagtg ggaaatcaat aaaatgatta
     5101 ttttatgaat atatttcatt gtgcaagtag atagaaatta catatgttac ataacacacg
     5161 aaataaacaa aaaaagacaa tccaaaaaca aacaccccaa aaaaaataat cactttagat
     5221 aaactcgtat gaggagaggc acgttcagtg actcgacgat tcccgagcaa aaaaagtctc
     5281 cccgtcacac atgtagtggg tgacgcaatt atctttaaag taatccttct gttgacttgt
     5341 cattgataac atccagtctt cgtcaggatt gcaaagaatt atagaaggga tcccaccttt
     5401 tattttcttc ttttttccat atttagggtt gacagtgaaa tcagactggc aacctattaa
     5461 ttgcttccac aatgggacga acttgaaggg gatgtcgtcg atgatattat aggtggcgtg
     5521 ttcatcgtag ttggtgaaat cgatggtacc gttccaatag ttgtgtcgtc cgagacttct
     5581 agcccaggtg gtctttccgg tacgagttgg tccgcagatg tagaggctgg ggtgtcggat
     5641 tccattcctt ccattgtcct tgttaaatcg gccatccatt caaggtcaga ttgagcttgt
     5701 tggtatgaga caggatgtat gtaagtataa gcgtctatgc ttacatggta tagatgggtt
     5761 tccctccagg agtgtagatc ttcgtggcag cgaagatctg attctgtgaa gggcgacaca
     5821 tacggttcag gttgtggagg gaataatttg ttggctgaat attccagcca ttgaagcttt
     5881 gttgcccatt catgagggaa ttcttccttg atcatgtcaa gatattcctc cttagacgtt
     5941 gcagtctgga taatagttct ccatcgtgcg tcagatttgc gaggagaaac cttatgatct
     6001 cggaaatctc ctctggtttt aatatctccg tcctttgata tgtaatcaag gacttgttta
     6061 gagtttctag ctggctggat attagggtga tttccttcaa aatcgaaaaa agaaggatcc
     6121 ctaatacaag gttttttatc aagctggaga agagcatgat agtgggtagt gccatcttga
     6181 tgaagctcag aagcaacacc aaggaagaaa ataagaaaag gtgtgagttt ctcccagaga
     6241 aactggaata aatcatctct ttgagatgag cacttgggat aggtaaggaa aacatattta
     6301 gattggagtc tgaagttctt actagcagaa ggcatgttgt tgtgactccg aggggttgcc
     6361 tcaaactcta tcttataacc ggcgtggagg catggaggca ggggtatttt ggtcatttta
     6421 atagatagtg gaaaatgacg tggaatttac ttaaagacga agtctttgcg acaagggggg
     6481 gcccacgccg aatttaatat taccggcgtg gccccccctt atcgcgagtg ctttagcacg
     6541 agcggtccag atttaaagta gaaaatttcc cgcccactag ggttaaaggt gttcacacta
     6601 taaaagcata tacgatgtga tggtatttga tggagcgtat attgtatcag gtatttccgt
     6661 tggatacgaa ttattcgtac gaccctcata gtttaaacta tcagtgtttg acaggatata
     6721 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     6781 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     6841 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     6901 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     6961 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     7021 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     7081 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     7141 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     7201 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     7261 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     7321 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     7381 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     7441 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     7501 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     7561 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     7621 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     7681 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     7741 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     7801 gcggccgggt acgtgttcga gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc
     7861 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     7921 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     7981 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     8041 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     8101 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     8161 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     8221 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     8281 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     8341 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     8401 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     8461 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     8521 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     8581 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     8641 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     8701 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     8761 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
     8821 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
     8881 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
     8941 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
     9001 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
     9061 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
     9121 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
     9181 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
     9241 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
     9301 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
     9361 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
     9421 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
     9481 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
     9541 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
     9601 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
     9661 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
     9721 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
     9781 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
     9841 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
     9901 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
     9961 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
    10021 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
    10081 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
    10141 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
    10201 aggggaaaaa ggtcgaaaag gcctctttcc tgtggatagc acgtacattg ggaacccaaa
    10261 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
    10321 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
    10381 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
    10441 cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
    10501 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
    10561 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
    10621 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10681 cggaaacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10741 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
    10801 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    10861 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
    10921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    11041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    11101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    11161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    11221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    11281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    11341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    11401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    11461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    11521 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    11581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    11641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    11701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgca
    11761 ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct cccaatcagg
    11821 cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga tatcctccct
    11881 gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc ttctcccaag
    11941 atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc
    12001 gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg
    12061 cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg ccagatcgtt
    12121 attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat agggacaatc
    12181 cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga taatcttttc
    12241 agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct cactcatgag
    12301 cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag gcagctttcc
    12361 ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc ctttataccg
    12421 gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat ataccttagc
    12481 aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt ttttcaattc
    12541 cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca gtatttaaag
    12601 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
    12661 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
    12721 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
    12781 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
    12841 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
    12901 cgctgacgcc gtcccgga
//
