LOCUS       sequence-254535-       15643 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, 
            sgRNA targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15643)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 15643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM113
FEATURES             Location/Qualifiers
     source          1..15643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        966..1311
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1254..1274
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1329..2981
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1395..1413)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2991..3239
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        3732..3807
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3910..4089
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3942..4125
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     polyA_signal    7508..7682
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9364..9388
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10689..11318
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11747..12820
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12886..13080
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13316..13338)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13424..13564
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13479..13498
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13579..13596)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13750..14338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13830..13849)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14425..15219)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgccag
      481 atttgccttt tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca
      541 gcaggtatca tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc
      601 aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa cacagagaaa
      661 gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg cttgcttcac
      721 aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac tgaatcaaag
      781 gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa gactggcgaa
      841 cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt caacatggtg
      901 gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga agaccaaagg
      961 gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt ccattgccca
     1021 gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta caaatgccat
     1081 cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg tcccaaagat
     1141 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     1201 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     1261 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac acgggggact
     1321 cctgcaggat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
     1381 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
     1441 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
     1501 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
     1561 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
     1621 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
     1681 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
     1741 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
     1801 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
     1861 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
     1921 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
     1981 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
     2041 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
     2101 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
     2161 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     2221 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     2281 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     2341 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     2401 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     2461 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     2521 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     2581 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     2641 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     2701 tgctccaaca ccccaacatc ttcgacgctg gtgtcgcagg tcttcccgac gatgacgccg
     2761 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     2821 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     2881 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     2941 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgtg acgtcgacga tatgaagatg
     3001 aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg tgtttttttc
     3061 ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg taagatcaca
     3121 ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg gatctcttct
     3181 gcagcatata actactgtat gtgctatggt atggactatg gaatatgatt aaagataagc
     3241 cagagctctg gtgacggacc catggcttcg ttgaacaacg gaaactcgac ttgccttccg
     3301 cacaatacat catttcttct tagctttttt tcttcttctt cgttcataca gttttttttt
     3361 gtttatcagc ttacattttc ttgaaccgta gctttcgttt tcttcttttt aactttccat
     3421 tcggagtttt tgtatcttgt ttcatagttt gtcccaggat tagaatgatt aggcatcgaa
     3481 ccttcaagaa tttgattgaa taaaacatct tcattcttaa gatatgaaga taatcttcaa
     3541 aaggcccctg ggaatctgaa agaagagaag caggcccatt tatatgggaa agaacaatag
     3601 tatttcttat ataggcccat ttaagttgaa aacaatcttc aaaagtccca catcgcttag
     3661 ataagaaaac gaagctgagt ttatatacag ctagagtcga agtagtgatt gttggtagta
     3721 gcgactccat ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
     3781 cttgaaaaag tggcaccgag tcggtgcttt ttttcccggg cgcgccgatc atgagcggag
     3841 aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt tttacgtttg
     3901 gaactgacag aaccgcaacg ttgaaggagc cactcagccg cgggtttctg gagtttaatg
     3961 agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta aggtcactat
     4021 cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat tcccctcggt
     4081 atccaattag agtctcatat tcactctcaa tccaaataat ctgcaccgta cctgcagggt
     4141 ccgagctagg tcacagaagc gctcaggaag gccgctgaga tagaggcatg gcggccaatg
     4201 cgggcggcgg tggagcggga ggaggcagcg gcagcggcag cgtggctgcg ccggcggtgt
     4261 gccgccccag cggctcgcgg tggacgccga cgccggagca gatcaggatg ctgaaggagc
     4321 tgtactacgg ctgcggcatc cggtcgccca gctcggagca gatccagcgc atcaccgcca
     4381 tgctgcggca gcacggcaag atcgagggca agaacgtctt ctactggttc cagaaccaca
     4441 aggcccgcga gcgccagaag cgccgcctca ccagcctcga cgtgaacgtg cccgccgccg
     4501 gcgcggccga cgccaccacc agccaactcg gcgtcctctc gctgtcgtcg ccgccgcctt
     4561 caggcgcggc gcctccctcg cccaccctcg gcttctacgc cgccggcaat ggcggcggat
     4621 cggctgtgct gctggacacg agttccgact ggggcagcag cggcgctgcg atggccaccg
     4681 agacatgctt cctccaggac tacatgggcg tgacggacac gggcagctcg tcgcagtggc
     4741 cacgcttctc gtcgtcggac acgataatgg cggcggccgc ggcgcgggcg gcgacgacgc
     4801 gggcgcccga gactctccct ctcttcccga cctgcggcga cgacggcggc agcggtagca
     4861 gcagctactt gccgttctgg ggtgccgcgt ccacaactgc cggcgccact tcttccgttg
     4921 cgatccagca gcaacaccag ctgcaggagc agtacagctt ttacagcaac agcaacagca
     4981 cccagctggc cggcaccggc aaccaagacg tatcggcaac agcagcagca gccgccgccc
     5041 tggagctgag cctcagctca tggtgctccc cttaccctgc tgcagggagt atgtgagagc
     5101 aacgcgagct gccactgctc ttcacttatg tctctggaat ggaaggagga ggaagtgagc
     5161 atagcgttgg tgcgttgctg tcattgtcct aggttagtag ctagtgccag ttactagtaa
     5221 gcatcaggca taggagtatg tagtagaagc atgcacgttg ccggccagcc aggctttaga
     5281 cgggaaaaga atttggtgca gccggctgca aaacaggatg tttacagccc ccccctcgag
     5341 ccctagactt gtccatcttc tggattggcc aagttaatta atgtatgaaa taaaaggatg
     5401 cacacatagt gacatgctaa tcactataat gtgggcatca aagttgtgtg ttatgtgtaa
     5461 ttactaatta tctgaataag agaaagagat catccatatt tcttatccta aatgaatgtc
     5521 acgtgtcttt ataattcttt gatgaaccag atgcatttta ttaaccaatt ccatatacat
     5581 ataaatatta atcatatata attaatatca attgggttag caaaacaaat ctagtctagg
     5641 tgtgttttgc taattattgg gggatagtgc aaaaagaaat ctacgttctc aataattcag
     5701 atagaaaact taataaagtg agataattta catagattgc ttttatcctt tgatatatgt
     5761 gaaaccatgc atgatataag gaaaatagat agagaaataa ttttttacat cgttgaatat
     5821 gtaaacaatt taattcaaga agctaggaat ataaatattg aggagtttat gattagagct
     5881 ctcccgctgg cagacatact gtcccacaaa tgaagatgga atctgtaaaa gaaaacgcgt
     5941 gaaataatgc gtctgacaaa ggttaggtcg gctgccttta atcaatacca aagtggtccc
     6001 taccacgatg gaaaaactgt gcagtcggtt tggctttttc tgacgaacaa ataagattcg
     6061 tggccgacag gtgggggtcc accatgtgaa ggcatcttca gactccaata atggagcaat
     6121 gacgtaaggg cttacgaaat aagtaagggt agtttgggaa atgtccactc acccgtcagt
     6181 ctataaatac ttagcccctc cctcattgtt aagggagcaa aatctcagag agatagtcct
     6241 agagagagaa agagagcaag tagcctagaa gtagtcaagg cggcgaagta ttcaggcacg
     6301 tggccaggaa gaagaaaagc caagacgacg aaaacaggta agagctaagc ttatggagag
     6361 tggttccaac agcacttctt gtccaatggc ttttgccggg gataatagtg atggtccgat
     6421 gtgtcctatg atgatgatga tgccgcccat catgacatca catcaacatc atggtcatga
     6481 tcatcaacat caacaacaag aacatgatgg ttatgcatat cagtcacacc accaacaaag
     6541 tagttccctt tttcttcaat cactagctcc tccccaagga actaagaaca aagttgcttc
     6601 ttcttcttct ccttcctctt gtgctcctgc ctattctcta atggagatcc atcataacga
     6661 aatcgttgca ggaggaatca acccttgctc ctcttcctct tcttcagcct ctgtcaaggc
     6721 caagatcatg gctcatcctc actaccaccg cctcttggcc gcttatgtca attgtcagaa
     6781 ggttggagca ccaccggagg ttgtggcgag gctagaggag gcatgctcgt ctgccgcagc
     6841 cgctgccgca tctatgggac caacaggatg tctaggtgaa gatccagggc ttgatcaatt
     6901 catggaagct tactgtgaaa tgctcgttaa gtatgagcaa gagctctcca aacctttcaa
     6961 ggaagctatg gtcttccttc aacgtgtcga gtgtcaattc aaatccctct ctctatcctc
     7021 accttcctct ttctccggtt atggagagac agcaattgat aggaacaata atgggtcatc
     7081 cgaggaagaa gtcgatatga acaatgaatt tgtagatcca caagctgagg atagagagct
     7141 taaaggacag ctcttgcgca agtacagtgg ttacttaggg agcctcaagc aagagttcat
     7201 gaagaagagg aagaaaggaa agctccctaa agaagctcgt caacaactgc ttgattggtg
     7261 gagccgtcac tacaaatggc cttacccttc ggagcaacaa aagctcgccc ttgcggaatc
     7321 aacggggctg gaccagaaac agataaacaa ttggttcata aaccagagga aacggcattg
     7381 gaagccgtcg gaggacatgc agtttgtagt aatggacgca acacatcctc accattactt
     7441 catggataat gtcttgggca atcctttccc aatggatcac atctcctcca ccatgctttg
     7501 actcgagttt ctccataata atgtgtgagt agttcccaga taagggaatt agggttccta
     7561 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa
     7621 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtact aaaatccaga
     7681 tcccccgaat taagtgtttg atcgccggcg gtaccgagtg tacttcaagt cagtgggaaa
     7741 tcaataaaat gattatttta tgaatatatt tcattgtgca agtagataga aattacatat
     7801 gttacataac acacgaaata aacaaaaaaa gacaatccaa aaacaaacac cccaaaaaaa
     7861 ataatcactt tagataaact cgtatgagga gaggcacgtt cagtgactcg acgattcccg
     7921 agcaaaaaaa gtctccccgt cacacatgta gtgggtgacg caattatctt taaagtaatc
     7981 cttctgttga cttgtcattg ataacatcca gtcttcgtca ggattgcaaa gaattataga
     8041 agggatccca ccttttattt tcttcttttt tccatattta gggttgacag tgaaatcaga
     8101 ctggcaacct attaattgct tccacaatgg gacgaacttg aaggggatgt cgtcgatgat
     8161 attataggtg gcgtgttcat cgtagttggt gaaatcgatg gtaccgttcc aatagttgtg
     8221 tcgtccgaga cttctagccc aggtggtctt tccggtacga gttggtccgc agatgtagag
     8281 gctggggtgt cggattccat tccttccatt gtccttgtta aatcggccat ccattcaagg
     8341 tcagattgag cttgttggta tgagacagga tgtatgtaag tataagcgtc tatgcttaca
     8401 tggtatagat gggtttccct ccaggagtgt agatcttcgt ggcagcgaag atctgattct
     8461 gtgaagggcg acacatacgg ttcaggttgt ggagggaata atttgttggc tgaatattcc
     8521 agccattgaa gctttgttgc ccattcatga gggaattctt ccttgatcat gtcaagatat
     8581 tcctccttag acgttgcagt ctggataata gttctccatc gtgcgtcaga tttgcgagga
     8641 gaaaccttat gatctcggaa atctcctctg gttttaatat ctccgtcctt tgatatgtaa
     8701 tcaaggactt gtttagagtt tctagctggc tggatattag ggtgatttcc ttcaaaatcg
     8761 aaaaaagaag gatccctaat acaaggtttt ttatcaagct ggagaagagc atgatagtgg
     8821 gtagtgccat cttgatgaag ctcagaagca acaccaagga agaaaataag aaaaggtgtg
     8881 agtttctccc agagaaactg gaataaatca tctctttgag atgagcactt gggataggta
     8941 aggaaaacat atttagattg gagtctgaag ttcttactag cagaaggcat gttgttgtga
     9001 ctccgagggg ttgcctcaaa ctctatctta taaccggcgt ggaggcatgg aggcaggggt
     9061 attttggtca ttttaataga tagtggaaaa tgacgtggaa tttacttaaa gacgaagtct
     9121 ttgcgacaag ggggggccca cgccgaattt aatattaccg gcgtggcccc cccttatcgc
     9181 gagtgcttta gcacgagcgg tccagattta aagtagaaaa tttcccgccc actagggtta
     9241 aaggtgttca cactataaaa gcatatacga tgtgatggta tttgatggag cgtatattgt
     9301 atcaggtatt tccgttggat acgaattatt cgtacgaccc tcatagttta aactatcagt
     9361 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     9421 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     9481 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     9541 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     9601 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     9661 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     9721 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     9781 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     9841 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     9901 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     9961 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
    10021 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
    10081 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
    10141 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
    10201 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
    10261 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
    10321 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
    10381 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
    10441 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg
    10501 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
    10561 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
    10621 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
    10681 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
    10741 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
    10801 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
    10861 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
    10921 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
    10981 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
    11041 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
    11101 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
    11161 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
    11221 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
    11281 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
    11341 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
    11401 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
    11461 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
    11521 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
    11581 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
    11641 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
    11701 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
    11761 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
    11821 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
    11881 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
    11941 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
    12001 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
    12061 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
    12121 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
    12181 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
    12241 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
    12301 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
    12361 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
    12421 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
    12481 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
    12541 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
    12601 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
    12661 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
    12721 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    12781 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    12841 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta
    12901 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    12961 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    13021 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    13081 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    13141 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    13201 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    13261 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    13321 gacacatgca gctcccggaa acggtcacag cttgtctgta agcggatgcc gggagcagac
    13381 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
    13441 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
    13501 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    13561 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    13621 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    13681 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    13741 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    13801 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    13861 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    13921 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    13981 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    14041 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    14101 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    14161 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    14221 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    14281 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    14341 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    14401 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    14461 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    14521 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    14581 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    14641 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    14701 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    14761 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    14821 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    14881 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    14941 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    15001 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    15061 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    15121 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    15181 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    15241 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    15301 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    15361 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca
    15421 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
    15481 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
    15541 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
    15601 attttgtgcc gagctgccgg tcggggagct gttggctggc tgg
//
