LOCUS       sequence-254539-       13474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, WUS2, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13474)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 13474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM135
FEATURES             Location/Qualifiers
     source          1..13474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             952..2604
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1018..1036)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2612..2860
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        3350..3425
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3528..3707
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3560..3743
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7195..7219
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8520..9149
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9578..10651
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10717..10911
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11147..11169)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11255..11395
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11310..11329
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11410..11427)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11581..12169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11661..11680)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12256..13050)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgcctg
      481 gcagacatac tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg
      541 cgtctgacaa aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat
      601 ggaaaaactg tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca
      661 ggtgggggtc caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg
      721 gcttacgaaa taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata
      781 cttagcccct ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga
      841 aagagagcaa gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga
      901 agaagaaaag ccaagacgac gaaaacaggt aagagctaag cttcctgcac catggaagac
      961 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
     1021 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     1081 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
     1141 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     1201 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     1261 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
     1321 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
     1381 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     1441 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
     1501 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
     1561 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
     1621 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     1681 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     1741 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa
     1801 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa
     1861 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc
     1921 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact
     1981 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     2041 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     2101 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac
     2161 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     2221 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag
     2281 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac
     2341 atcttcgacg ctggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     2401 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     2461 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     2521 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     2581 aagggcggaa agatcgccgt gtaactcgag atatgaagat gaagatgaaa tatttggtgt
     2641 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     2701 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
     2761 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     2821 tgtgctatgg tatggactat ggaatatgat taaagataag gagctccggt gacggaccca
     2881 tggcttcgtt gaacaacgga aactcgactt gccttccgca caatacatca tttcttctta
     2941 gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt acattttctt
     3001 gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg tatcttgttt
     3061 catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt tgattgaata
     3121 aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg aatctgaaag
     3181 aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat aggcccattt
     3241 aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga agctgagttt
     3301 atatacagct agagtcgaag tagtgattgt tggtagtagc gactccatgg ttttagagct
     3361 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     3421 ggtgcttttt ttcccgggcg cgccgatcat gagcggagaa ttaagggagt cacgttatga
     3481 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
     3541 gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     3601 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     3661 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     3721 actctcaatc caaataatct gcaccgtacc tgcagggtcc gagctaggtc acagaagcgc
     3781 tcaggaaggc cgctgagata gaggcatggc ggccaatgcg ggcggcggtg gagcgggagg
     3841 aggcagcggc agcggcagcg tggctgcgcc ggcggtgtgc cgccccagcg gctcgcggtg
     3901 gacgccgacg ccggagcaga tcaggatgct gaaggagctg tactacggct gcggcatccg
     3961 gtcgcccagc tcggagcaga tccagcgcat caccgccatg ctgcggcagc acggcaagat
     4021 cgagggcaag aacgtcttct actggttcca gaaccacaag gcccgcgagc gccagaagcg
     4081 ccgcctcacc agcctcgacg tgaacgtgcc cgccgccggc gcggccgacg ccaccaccag
     4141 ccaactcggc gtcctctcgc tgtcgtcgcc gccgccttca ggcgcggcgc ctccctcgcc
     4201 caccctcggc ttctacgccg ccggcaatgg cggcggatcg gctgtgctgc tggacacgag
     4261 ttccgactgg ggcagcagcg gcgctgcgat ggccaccgag acatgcttcc tccaggacta
     4321 catgggcgtg acggacacgg gcagctcgtc gcagtggcca cgcttctcgt cgtcggacac
     4381 gataatggcg gcggccgcgg cgcgggcggc gacgacgcgg gcgcccgaga ctctccctct
     4441 cttcccgacc tgcggcgacg acggcggcag cggtagcagc agctacttgc cgttctgggg
     4501 tgccgcgtcc acaactgccg gcgccacttc ttccgttgcg atccagcagc aacaccagct
     4561 gcaggagcag tacagctttt acagcaacag caacagcacc cagctggccg gcaccggcaa
     4621 ccaagacgta tcggcaacag cagcagcagc cgccgccctg gagctgagcc tcagctcatg
     4681 gtgctcccct taccctgctg cagggagtat gtgagagcaa cgcgagctgc cactgctctt
     4741 cacttatgtc tctggaatgg aaggaggagg aagtgagcat agcgttggtg cgttgctgtc
     4801 attgtcctag gttagtagct agtgccagtt actagtaagc atcaggcata ggagtatgta
     4861 gtagaagcat gcacgttgcc ggccagccag gctttagacg ggaaaagaat ttggtgcagc
     4921 cggctgcaaa acaggatgtt tacagccccc ccctcgagcc ctagacttgt ccatcttctg
     4981 gattggccaa gttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc
     5041 actataatgt gggcatcaaa gttgtgtgtt atgtgtaatt actaattatc tgaataagag
     5101 aaagagatca tccatatttc ttatcctaaa tgaatgtcac gtgtctttat aattctttga
     5161 tgaaccagat gcattttatt aaccaattcc atatacatat aaatattaat catatataat
     5221 taatatcaat tgggttagca aaacaaatct agtctaggtg tgttttgcta attattgggg
     5281 gatagtgcaa aaagaaatct acgttctcaa taattcagat agaaaactta ataaagtgag
     5341 ataatttaca tagattgctt ttatcctttg atatatgtga aaccatgcat gatataagga
     5401 aaatagatag agaaataatt ttttacatcg ttgaatatgt aaacaattta attcaagaag
     5461 ctaggaatat aaatattgag gagtttatga ttagagctct cccggcgcgc cgatatcgag
     5521 ctcagtgttt gatcgccggc ggtaccgagt gtacttcaag tcagtgggaa atcaataaaa
     5581 tgattatttt atgaatatat ttcattgtgc aagtagatag aaattacata tgttacataa
     5641 cacacgaaat aaacaaaaaa agacaatcca aaaacaaaca ccccaaaaaa aataatcact
     5701 ttagataaac tcgtatgagg agaggcacgt tcagtgactc gacgattccc gagcaaaaaa
     5761 agtctccccg tcacacatgt agtgggtgac gcaattatct ttaaagtaat ccttctgttg
     5821 acttgtcatt gataacatcc agtcttcgtc aggattgcaa agaattatag aagggatccc
     5881 accttttatt ttcttctttt ttccatattt agggttgaca gtgaaatcag actggcaacc
     5941 tattaattgc ttccacaatg ggacgaactt gaaggggatg tcgtcgatga tattataggt
     6001 ggcgtgttca tcgtagttgg tgaaatcgat ggtaccgttc caatagttgt gtcgtccgag
     6061 acttctagcc caggtggtct ttccggtacg agttggtccg cagatgtaga ggctggggtg
     6121 tcggattcca ttccttccat tgtccttgtt aaatcggcca tccattcaag gtcagattga
     6181 gcttgttggt atgagacagg atgtatgtaa gtataagcgt ctatgcttac atggtataga
     6241 tgggtttccc tccaggagtg tagatcttcg tggcagcgaa gatctgattc tgtgaagggc
     6301 gacacatacg gttcaggttg tggagggaat aatttgttgg ctgaatattc cagccattga
     6361 agctttgttg cccattcatg agggaattct tccttgatca tgtcaagata ttcctcctta
     6421 gacgttgcag tctggataat agttctccat cgtgcgtcag atttgcgagg agaaacctta
     6481 tgatctcgga aatctcctct ggttttaata tctccgtcct ttgatatgta atcaaggact
     6541 tgtttagagt ttctagctgg ctggatatta gggtgatttc cttcaaaatc gaaaaaagaa
     6601 ggatccctaa tacaaggttt tttatcaagc tggagaagag catgatagtg ggtagtgcca
     6661 tcttgatgaa gctcagaagc aacaccaagg aagaaaataa gaaaaggtgt gagtttctcc
     6721 cagagaaact ggaataaatc atctctttga gatgagcact tgggataggt aaggaaaaca
     6781 tatttagatt ggagtctgaa gttcttacta gcagaaggca tgttgttgtg actccgaggg
     6841 gttgcctcaa actctatctt ataaccggcg tggaggcatg gaggcagggg tattttggtc
     6901 attttaatag atagtggaaa atgacgtgga atttacttaa agacgaagtc tttgcgacaa
     6961 gggggggccc acgccgaatt taatattacc ggcgtggccc ccccttatcg cgagtgcttt
     7021 agcacgagcg gtccagattt aaagtagaaa atttcccgcc cactagggtt aaaggtgttc
     7081 acactataaa agcatatacg atgtgatggt atttgatgga gcgtatattg tatcaggtat
     7141 ttccgttgga tacgaattat tcgtacgacc ctcatagttt aaactatcag tgtttgacag
     7201 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
     7261 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
     7321 ccctcgggat caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg
     7381 acgttcagtg cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca
     7441 ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta
     7501 gaatacttgc gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct
     7561 gctgggctat gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact
     7621 gcacgcggcc ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg
     7681 cccggagctg gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag
     7741 gctagaccgc ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga
     7801 ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg
     7861 ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga
     7921 ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc
     7981 taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac
     8041 cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga
     8101 gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc
     8161 attgaccgag gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg
     8221 aaaccgcacc aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag
     8281 atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg gctcaaccgt gcggctgcat
     8341 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     8401 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     8461 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     8521 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     8581 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     8641 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     8701 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     8761 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     8821 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     8881 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     8941 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     9001 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     9061 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     9121 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     9181 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     9241 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     9301 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     9361 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     9421 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     9481 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgcc tgccggccct
     9541 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     9601 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     9661 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     9721 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     9781 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     9841 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     9901 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     9961 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
    10021 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
    10081 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
    10141 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
    10201 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
    10261 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
    10321 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
    10381 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
    10441 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
    10501 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
    10561 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
    10621 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
    10681 cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg gatagcacgt acattgggaa
    10741 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
    10801 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
    10861 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
    10921 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
    10981 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
    11041 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
    11101 acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
    11161 agctcccgga aacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
    11221 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
    11281 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
    11341 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
    11401 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11461 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11521 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11581 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11641 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11701 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11761 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11821 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11881 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11941 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    12001 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    12061 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12121 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12181 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12241 catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca
    12301 atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc
    12361 ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct
    12421 cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag
    12481 gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag
    12541 ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag
    12601 atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg
    12661 acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat
    12721 cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact
    12781 catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag
    12841 ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt
    12901 ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac
    12961 cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt
    13021 caattccggt gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat
    13081 ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact gttccttgca
    13141 ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat
    13201 aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt
    13261 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
    13321 tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg
    13381 ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc
    13441 cgagctgccg gtcggggagc tgttggctgg ctgg
//
