LOCUS       sequence-25454-e         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC97
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..46)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(68..88)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(110..130)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(152..172)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    211..258
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    297..421
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(564..583)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cncctttact cattttttct accggtacct tacgcttctt ctttggagca gtcatttttt
       61 ctgccggtac cttacgcttc ttctttggag cagtcatttt ttctgccggt accttacgct
      121 tcttctttgg agcagtcatt ttttctgccg gtaccttacg cttcttcttt ggagcagtca
      181 ttttttctga gctcggtacc cggggatctt gaagttccta ttccgaagtt cctattctct
      241 agaaagtata ggaacttcag agcgcttttg aagctagaac tagtggatct gatatcacca
      301 ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      361 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      421 ggtcgacctg cagactggct gtgtataagg gagcctgaca tttatattcc ccagaacatc
      481 aggttaatgg cgtttttgat gtcattttcg cggtggctga gatcagccac ttcttccccg
      541 ataacggaga ccggcacact ggccatatcg gtggtcatca tgcgccagct ttcatccccg
      601 atatgcacca ccgggtaaag ttcacgggag actttatctg acagcagacg tgcactggcc
      661 agggggatca ccatccgtcg cccggggcgt gtcnataata tcactctgta cntcc
//
