LOCUS       sequence-25455-e         655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC183
FEATURES             Location/Qualifiers
     source          1..655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..49)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(71..91)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(113..133)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(155..175)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    214..261
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    300..424
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(567..586)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cttctccttt actcattttt tctaccggta ccttacgctt cttctttgga gcagtcattt
       61 tttctgccgg taccttacgc ttcttctttg gagcagtcat tttttctgcc ggtaccttac
      121 gcttcttctt tggagcagtc attttttctg ccggtacctt acgcttcttc tttggagcag
      181 tcattttttc tgagctcggt acccggggat cttgaagttc ctattccgaa gttcctattc
      241 tctagaaagt ataggaactt cagagcgctt ttgaagctag aactagtgga tctgatatca
      301 ccactttgta caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      361 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
      421 tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
      481 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
      541 ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
      601 ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacaccag acgtg
//
