LOCUS       sequence-254566-       13548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BeYDV replicon with constitutive Luc expression, IPT, sgRNA 
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13548)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 13548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2."
COMMENT     Sequence Label: pMM134
FEATURES             Location/Qualifiers
     source          1..13548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             927..2579
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(993..1011)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      2587..2835
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        3325..3400
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3916..4261
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4204..4224
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7244..7268
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8569..9198
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9627..10700
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10766..10960
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11196..11218)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11304..11444
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11359..11378
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11459..11476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11630..12218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11710..11729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12305..13099)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13524..13548
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta gagctcaaag
       61 tttaacgcgt tagcagaagg catgttgttg tgactccgag gggttgcctc aaactctatc
      121 ttataaccgg cgtggaggca tggaggcagg ggtattttgg tcattttaat agatagtgga
      181 aaatgacgtg gaatttactt aaagacgaag tctttgcgac aagggggggc ccacgccgaa
      241 tttaatatta ccggcgtggc ccccccttat cgcgagtgct ttagcacgag cggtccagat
      301 ttaaagtaga aaatttcccg cccactaggg ttaaaggtgt tcacactata aaagcatata
      361 cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt atttccgttg gatacgaatt
      421 attcgtacga ccctcggtac cgatcggcgc gcctggcaga catactgtcc cacaaatgaa
      481 gatggaatct gtaaaagaaa acgcgtgaaa taatgcgtct gacaaaggtt aggtcggctg
      541 cctttaatca ataccaaagt ggtccctacc acgatggaaa aactgtgcag tcggtttggc
      601 tttttctgac gaacaaataa gattcgtggc cgacaggtgg gggtccacca tgtgaaggca
      661 tcttcagact ccaataatgg agcaatgacg taagggctta cgaaataagt aagggtagtt
      721 tgggaaatgt ccactcaccc gtcagtctat aaatacttag cccctccctc attgttaagg
      781 gagcaaaatc tcagagagat agtcctagag agagaaagag agcaagtagc ctagaagtag
      841 tcaaggcggc gaagtattca ggcacgtggc caggaagaag aaaagccaag acgacgaaaa
      901 caggtaagag ctaagcttcc tgcaccatgg aagacgccaa aaacataaag aaaggcccgg
      961 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
     1021 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
     1081 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
     1141 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
     1201 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
     1261 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
     1321 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
     1381 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
     1441 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
     1501 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
     1561 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
     1621 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
     1681 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
     1741 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
     1801 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
     1861 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
     1921 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
     1981 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
     2041 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
     2101 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
     2161 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
     2221 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
     2281 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgctggt gtcgcaggtc
     2341 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
     2401 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
     2461 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
     2521 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaac
     2581 tcgagatatg aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta
     2641 agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa
     2701 tgaatgtaag atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt
     2761 ttgttggatc tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat
     2821 atgattaaag ataaggagct ccggtgacgg acccatggct tcgttgaaca acggaaactc
     2881 gacttgcctt ccgcacaata catcatttct tcttagcttt ttttcttctt cttcgttcat
     2941 acagtttttt tttgtttatc agcttacatt ttcttgaacc gtagctttcg ttttcttctt
     3001 tttaactttc cattcggagt ttttgtatct tgtttcatag tttgtcccag gattagaatg
     3061 attaggcatc gaaccttcaa gaatttgatt gaataaaaca tcttcattct taagatatga
     3121 agataatctt caaaaggccc ctgggaatct gaaagaagag aagcaggccc atttatatgg
     3181 gaaagaacaa tagtatttct tatataggcc catttaagtt gaaaacaatc ttcaaaagtc
     3241 ccacatcgct tagataagaa aacgaagctg agtttatata cagctagagt cgaagtagtg
     3301 attgttggta gtagcgactc catggtttta gagctagaaa tagcaagtta aaataaggct
     3361 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttccc ggcgtaatat
     3421 ggcgcgccag atttgccttt tcaatttcag aaagaatgct aacccacaga tggttagaga
     3481 ggcttacgca gcaggtatca tcaagacgat ctacccgagc aataatctcc aggaaatcaa
     3541 ataccttccc aagaaggtta aagatgcagt caaaagattc aggactaact gcatcaagaa
     3601 cacagagaaa gatatatttc tcaagatcag aagtactatt ccagtatgga cgattcaagg
     3661 cttgcttcac aaaccaaggc aagtaataga gattggagtc tctaaaaagg tagttcccac
     3721 tgaatcaaag gccatggagt caaagattca aatagaggac ctaacagaac tcgccgtaaa
     3781 gactggcgaa cagttcatac agagtctctt acgactcaat gacaagaaga aaatcttcgt
     3841 caacatggtg gagcacgaca cacttgtcta ctccaaaaat atcaaagata cagtctcaga
     3901 agaccaaagg gcaattgaga cttttcaaca aagggtaata tccggaaacc tcctcggatt
     3961 ccattgccca gctatctgtc actttattgt gaagatagtg gaaaaggaag gtggctccta
     4021 caaatgccat cattgcgata aaggaaaggc catcgttgaa gatgcctctg ccgacagtgg
     4081 tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac
     4141 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     4201 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagagaac
     4261 acgggggact cctgcaggat ggatctgcgt ctaattttcg gtccaacttg cacaggaaag
     4321 acgtcgaccg cgatacgtct tgcccagcag actggccttc cagtcctttc gctcgatcgg
     4381 gtccaatgct gtcctcaact gtcaaccgga agcggacgac caacagtgga agaactgaaa
     4441 ggaacgaccc gtctatacct tgaagatcgg cctctggtga agggtatcat cgcagccaag
     4501 caagctcacg aaaggctgat cggggaagtg tacaattatg aggcccacgg cgggcttatt
     4561 cttgagggag gatctatctc gttgctcagg tgcatggcgc aaagcagtta ttggagtacc
     4621 gattttcgtt ggcatattat tcgccacaag ttagcagacg aggagacatt catgaacgcg
     4681 gccaaggcca gagttaggca gatgttgcgc cctgctgtag gcccatctat tattcaagag
     4741 ttggttcatc tttggaatga gcctcggctg aggcccatac tgaaagagat cgacggatat
     4801 cgatatgcca tgttatttgc tagccagaac cagatcacac ccgatatgct attgcagctt
     4861 gacccagata tggagggtga gttgattcat ggaatcgctc aggagtatct catccatgcg
     4921 cgccggcagg agcaggaatt ccctccagtg agcgtggtcg ctttcgaagg attcgaaggt
     4981 ccaccgttcg gaatgtgcta gctcgagccc tagacttgtc catcttctgg attggccaag
     5041 ttaattaatg tatgaaataa aaggatgcac acatagtgac atgctaatca ctataatgtg
     5101 ggcatcaaag ttgtgtgtta tgtgtaatta ctaattatct gaataagaga aagagatcat
     5161 ccatatttct tatcctaaat gaatgtcacg tgtctttata attctttgat gaaccagatg
     5221 cattttatta accaattcca tatacatata aatattaatc atatataatt aatatcaatt
     5281 gggttagcaa aacaaatcta gtctaggtgt gttttgctaa ttattggggg atagtgcaaa
     5341 aagaaatcta cgttctcaat aattcagata gaaaacttaa taaagtgaga taatttacat
     5401 agattgcttt tatcctttga tatatgtgaa accatgcatg atataaggaa aatagataga
     5461 gaaataattt tttacatcgt tgaatatgta aacaatttaa ttcaagaagc taggaatata
     5521 aatattgagg agtttatgat tagagctctc ccggcgcgcc gatatcgagc tcagtgtttg
     5581 atcgccggcg gtaccgagtg tacttcaagt cagtgggaaa tcaataaaat gattatttta
     5641 tgaatatatt tcattgtgca agtagataga aattacatat gttacataac acacgaaata
     5701 aacaaaaaaa gacaatccaa aaacaaacac cccaaaaaaa ataatcactt tagataaact
     5761 cgtatgagga gaggcacgtt cagtgactcg acgattcccg agcaaaaaaa gtctccccgt
     5821 cacacatgta gtgggtgacg caattatctt taaagtaatc cttctgttga cttgtcattg
     5881 ataacatcca gtcttcgtca ggattgcaaa gaattataga agggatccca ccttttattt
     5941 tcttcttttt tccatattta gggttgacag tgaaatcaga ctggcaacct attaattgct
     6001 tccacaatgg gacgaacttg aaggggatgt cgtcgatgat attataggtg gcgtgttcat
     6061 cgtagttggt gaaatcgatg gtaccgttcc aatagttgtg tcgtccgaga cttctagccc
     6121 aggtggtctt tccggtacga gttggtccgc agatgtagag gctggggtgt cggattccat
     6181 tccttccatt gtccttgtta aatcggccat ccattcaagg tcagattgag cttgttggta
     6241 tgagacagga tgtatgtaag tataagcgtc tatgcttaca tggtatagat gggtttccct
     6301 ccaggagtgt agatcttcgt ggcagcgaag atctgattct gtgaagggcg acacatacgg
     6361 ttcaggttgt ggagggaata atttgttggc tgaatattcc agccattgaa gctttgttgc
     6421 ccattcatga gggaattctt ccttgatcat gtcaagatat tcctccttag acgttgcagt
     6481 ctggataata gttctccatc gtgcgtcaga tttgcgagga gaaaccttat gatctcggaa
     6541 atctcctctg gttttaatat ctccgtcctt tgatatgtaa tcaaggactt gtttagagtt
     6601 tctagctggc tggatattag ggtgatttcc ttcaaaatcg aaaaaagaag gatccctaat
     6661 acaaggtttt ttatcaagct ggagaagagc atgatagtgg gtagtgccat cttgatgaag
     6721 ctcagaagca acaccaagga agaaaataag aaaaggtgtg agtttctccc agagaaactg
     6781 gaataaatca tctctttgag atgagcactt gggataggta aggaaaacat atttagattg
     6841 gagtctgaag ttcttactag cagaaggcat gttgttgtga ctccgagggg ttgcctcaaa
     6901 ctctatctta taaccggcgt ggaggcatgg aggcaggggt attttggtca ttttaataga
     6961 tagtggaaaa tgacgtggaa tttacttaaa gacgaagtct ttgcgacaag ggggggccca
     7021 cgccgaattt aatattaccg gcgtggcccc cccttatcgc gagtgcttta gcacgagcgg
     7081 tccagattta aagtagaaaa tttcccgccc actagggtta aaggtgttca cactataaaa
     7141 gcatatacga tgtgatggta tttgatggag cgtatattgt atcaggtatt tccgttggat
     7201 acgaattatt cgtacgaccc tcatagttta aactatcagt gtttgacagg atatattggc
     7261 gggtaaacct aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa
     7321 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     7381 aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc
     7441 agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc
     7501 tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg
     7561 actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg
     7621 cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg
     7681 gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg
     7741 ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc
     7801 tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg
     7861 gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt
     7921 tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga
     7981 gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc
     8041 cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg
     8101 cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg
     8161 aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg
     8221 ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca
     8281 ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc
     8341 cgggtacgtg ttcgagccgc ccgcgcacgg ctcaaccgtg cggctgcatg aaatcctggc
     8401 cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga
     8461 gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc
     8521 tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc
     8581 gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc
     8641 gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc
     8701 cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg
     8761 acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg
     8821 ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg
     8881 gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag
     8941 cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa
     9001 ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt
     9061 gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt
     9121 cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt
     9181 aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc
     9241 taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag
     9301 acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga
     9361 agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc
     9421 agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga
     9481 ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga
     9541 ggaacgggcg gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact
     9601 ggaaccccca agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa
     9661 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
     9721 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
     9781 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
     9841 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
     9901 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
     9961 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
    10021 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
    10081 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
    10141 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
    10201 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
    10261 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
    10321 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
    10381 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
    10441 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
    10501 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
    10561 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
    10621 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
    10681 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
    10741 aaaaaggtcg aaaaggcctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
    10801 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
    10861 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
    10921 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
    10981 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
    11041 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
    11101 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
    11161 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggaa
    11221 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    11281 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    11341 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    11401 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    11461 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    11521 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    11581 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    11641 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    11701 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11761 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11821 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11881 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11941 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12001 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12061 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12121 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12181 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    12241 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    12301 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    12361 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    12421 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    12481 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    12541 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    12601 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    12661 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    12721 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    12781 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    12841 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    12901 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    12961 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    13021 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    13081 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    13141 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    13201 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    13261 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    13321 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    13381 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    13441 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    13501 tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaac
//
