LOCUS       sequence-25458-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC162
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..45)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(67..87)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(109..129)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(151..171)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    210..257
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    296..420
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(563..582)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tcctttactc nttttttcta ccggtacctt acgcttcttc tttggagcag tcattttttc
       61 tgccggtacc ttacgcttct tctttggagc agtcattttt tctgccggta ccttacgctt
      121 cttctttgga gcagtcattt tttctgccgg taccttacgc ttcttctttg gagcagtcat
      181 tttttctgag ctcggtaccc ggggatcttg aagttcctat tccgaagttc ctattctcta
      241 gaaagtatag gaacttcaga gcgcttttga agctagaact agtggatctg atatcaccac
      301 tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      361 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      421 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
      481 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
      541 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
      601 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagangt gcactggcca
      661 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaacn
      721 nnngataacg gctctctctt tnnnnnggtg naaaccttaa actgca
//
