LOCUS       sequence-25459-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC247
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..47)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(69..89)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(111..131)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(153..173)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    249..296
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    306..430
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(573..592)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tctcctttac tcnttttttc taccggtacc ttacgcttct tctttggagc agtcattttt
       61 tctgccggta ccttacgctt cttctttgga gcagtcattt tttctgccgg taccttacgc
      121 ttcttctttg gagcagtcat tttttctgcc ggtaccttac gcttcttctt tggagcagtc
      181 attttttctg agctcctgaa aatgttctat gttatgttag tatcattcga aacatacccg
      241 gggatcttga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttcagag
      301 catcaaccac tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata
      361 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
      421 agtcactatg gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
      481 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
      541 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
      601 tcatccccga tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt
      661 gcactggncn nggggatcac catccgtcgc ccgggcgtgt cnataatatc actctgtaca
      721 tccncaaaca nncgat
//
