LOCUS       sequence-254597-       15815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a derivative encoding E. coli core RNA polymerase subunits: 
            RpoA, B, C-ppx(His)10, Z.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15815)
  AUTHORS   Twist KA, Husnain SI, Franke JD, Jain D, Campbell EA, Nickels BE, 
            Thomas MS, Darst SA, Westblade LF
  TITLE     A novel method for the production of in vivo-assembled, recombinant 
            Escherichia coli RNA polymerase lacking the alpha C-terminal domain.
  JOURNAL   Protein Sci. 2011 Jun;20(6):986-95. doi: 10.1002/pro.622. Epub 2011 
            Apr 26.
  PUBMED    21416542
REFERENCE   2  (bases 1 to 15815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.622"; 
            journalName: "Protein Sci"; date: "2011-06"; volume: "20"; issue: 
            "6"; pages: "986-95"
COMMENT     Sequence Label: pEcRNAP6
FEATURES             Location/Qualifiers
     source          1..15815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(140..208,209..1000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(358..377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1042..1555)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1237..1258)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1449..1468
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1666..2254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2155..2174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2408..2425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2447..2466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2607..2629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2623..2814)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3371..4453)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4415..4434
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4454..4531)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4737..4756
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4840..4859
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4840..4858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4859..4883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4898..4920
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             14340..14363
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             14373..14402
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             15226..15237
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             15347..15502
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             15533..15808
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
       61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      301 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct
      361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
      421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
      961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag
     1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
     1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2401 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg
     2461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2641 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
     2701 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     2761 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     2821 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     2881 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     2941 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc
     3001 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     3061 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     3121 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     3181 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     3241 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     3301 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
     3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
     3481 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
     3541 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
     3601 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
     3661 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
     3721 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
     3781 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
     3841 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
     3901 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     3961 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     4021 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     4081 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     4141 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     4201 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     4261 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     4321 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     4381 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     4441 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     4501 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
     4561 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     4621 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     4681 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     4741 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     4801 cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg
     4861 aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga
     4921 tatacatatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg atatcgagca
     4981 agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct ttggccatac
     5041 tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg cggtgaccga
     5101 ggttgagatt gatggtgtac tacatgagta cagcacaaaa gaaggcgttc aggaagatat
     5161 cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca aagatgaagt
     5221 tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata tcacccacga
     5281 cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc cacctgaccg atgagaacgc
     5341 gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg cttctacccg
     5401 aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg catgctacag
     5461 ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc gtaccgacct
     5521 ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag aggcgattcg
     5581 tcgtgcggca accattctgg ctgaacaact ggaggctttc gttgacttac gtgatgtacg
     5641 tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc gccctgttga
     5701 cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta tccactatat
     5761 cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa
     5821 atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct
     5881 ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatccccgat ccgtggactt
     5941 gtcagcgact gaggaaacag accatggatc cccgatccgt cgacttgtca gcgagctgag
     6001 gaaccctatg gtttactcct ataccgagaa aaaacgtatt cgtaaggatt ttggtaaacg
     6061 tccacaagtt ctggatgtac cttatctcct ttctatccag cttgactcgt ttcagaaatt
     6121 tatcgagcaa gatcctgaag ggcagtatgg tctggaagct gctttccgtt ccgtattccc
     6181 gattcagagc tacagcggta attccgagct gcaatacgtc agctaccgcc ttggcgaacc
     6241 ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc tattccgcac cgctgcgcgt
     6301 taaactgcgt ctggtgatct atgagcgcga agcgccggaa ggcaccgtaa aagacattaa
     6361 agaacaagaa gtctacatgg gcgaaattcc gctcatgaca gacaacggta cctttgttat
     6421 caacggtact gagcgtgtta tcgtttccca gctgcaccgt agtccgggcg tcttctttga
     6481 ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg tataacgcgc gtatcatccc
     6541 ttaccgtggt tcctggctgg acttcgaatt cgatccgaag gacaacctgt tcgtacgtat
     6601 cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc gccctgaact acaccacaga
     6661 gcagatcctc gacctgttct ttgaaaaagt tatctttgaa atccgtgata acaagctgca
     6721 gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca tcttttgaca tcgaagctaa
     6781 cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg cgccacattc gccagctgga
     6841 aaaagacgac gtcaaactga tcgaagtccc ggttgagtac atcgcaggta aagtggttgc
     6901 taaagactat attgatgagt ctaccggcga gctgatctgc gcagcgaaca tggagctgag
     6961 cctggatctg ctggctaagc tgagccagtc tggtcacaag cgtatcgaaa cgctgttcac
     7021 caacgatctg gatcacggcc catatatctc tgaaacctta cgtgtcgacc caactaacga
     7081 ccgtctgagc gcactggtag aaatctaccg catgatgcgc cctggcgagc cgccgactcg
     7141 tgaagcagct gaaagcctgt tcgagaacct gttcttctcc gaagaccgtt atgacttgtc
     7201 tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc gaagaaatcg aaggttccgg
     7261 tatcctgagc aaagacgaca tcattgatgt tatgaaaaag ctcatcgata tccgtaacgg
     7321 taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt cgtatccgtt ccgttggcga
     7381 aatggcggaa aaccagttcc gcgttggcct ggtacgtgta gagcgtgcgg tgaaagagcg
     7441 tctgtctctg ggcgatctgg ataccctgat gccacaggat atgatcaacg ccaagccgat
     7501 ttccgcagca gtgaaagagt tcttcggttc cagccagctg tctcagttta tggaccagaa
     7561 caacccgctg tctgagatta cgcacaaacg tcgtatctcc gcactcggcc caggcggtct
     7621 gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac ccgactcact acggtcgcgt
     7681 atgtccaatc gaaacccctg aaggtccgaa catcggtctg atcaactctc tgtccgtgta
     7741 cgcacagact aacgaatacg gcttccttga gactccgtat cgtaaagtga ccgacggtgt
     7801 tgtaactgac gaaattcact acctgtctgc tatcgaagaa ggcaactacg ttatcgccca
     7861 ggcgaactcc aacttggatg aagaaggcca cttcgtagaa gacctggtaa cttgccgtag
     7921 caaaggcgaa tccagcttgt tcagccgcga ccaggttgac tacatggacg tatccaccca
     7981 gcaggtggta tccgtcggtg cgtccctgat cccgttcctg gaacacgatg acgccaaccg
     8041 tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg actctgcgcg ctgataagcc
     8101 gctggttggt actggtatgg aacgtgctgt tgccgttgac tccggtgtaa ctgcggtagc
     8161 taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt atcgttatca aagttaacga
     8221 agacgagatg tatccgggtg aagcaggtat cgacatctac aacctgacca aatacacccg
     8281 ttctaaccag aacacctgta tcaaccagat gccgtgtgtg tctctgggtg aaccggttga
     8341 acgtggcgac gtgctggcag acggtccgtc caccgacctc ggtgaactgg cgcttggtca
     8401 gaacatgcgc gtagcgttca tgccgtggaa tggttacaac ttcgaagact ccatcctcgt
     8461 atccgagcgt gttgttcagg aagaccgttt caccaccatc cacattcagg aactggcgtg
     8521 tgtgtcccgt gacaccaagc tgggtccgga agagatcacc gctgacatcc cgaacgtggg
     8581 tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt tacattggtg cggaagtgac
     8641 cggtggcgac attctggttg gtaaggtaac gccgaaaggt gaaactcagc tgaccccaga
     8701 agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct gacgttaaag actcttctct
     8761 gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt caggtcttta ctcgcgatgg
     8821 cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg cagctcaaac aggcgaagaa
     8881 agacctgtct gaagaactgc agatcctcga agcgggtctg ttcagccgta tccgtgctgt
     8941 gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa ctgccgcgcg atcgctggct
     9001 ggagctgggc ctgacagacg aagagaaaca aaatcagctg gaacagctgg ctgagcagta
     9061 tgacgaactg aaacacgagt tcgagaagaa actcgaagcg aaacgccgca aaatcaccca
     9121 gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag gtatatctgg cggttaaacg
     9181 ccgtatccag cctggtgaca agatggcagg tcgtcacggt aacaagggtg taatttctaa
     9241 gatcaacccg atcgaagata tgccttacga tgaaaacggt acgccggtag acatcgtact
     9301 gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag atcctcgaaa cccacctggg
     9361 tatggctgcg aaaggtatcg gcgacaagat caacgccatg ctgaaacagc agcaagaagt
     9421 cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg ggcgctgacg ttcgtcagaa
     9481 agttgacctg agtaccttca gcgatgaaga agttatgcgt ctggctgaaa acctgcgcaa
     9541 aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa gaagcagaaa ttaaagagct
     9601 gctgaaactt ggcgacctgc cgacttccgg tcagatccgc ctgtacgatg gtcgcactgg
     9661 tgaacagttc gagcgtccgg taaccgttgg ttacatgtac atgctgaaac tgaaccacct
     9721 ggtcgacgac aagatgcacg cgcgttccac cggttcttac agcctggtta ctcagcagcc
     9781 gctgggtggt aaggcacagt tcggtggtca gcgtttcggg gagatggaag tgtgggcgct
     9841 ggaagcatac ggcgcagcat acaccctgca ggaaatgctc accgttaagt ctgatgacgt
     9901 gaacggtcgt accaagatgt ataaaaacat cgtggacggc aaccatcaga tggagccggg
     9961 catgccagaa tccttcaacg tattgttgaa agagattcgt tcgctgggta tcaacatcga
    10021 actggaagac gagtaattct cgctcaaaca ggtcactgct gtcgggttaa aacccggcag
    10081 cggattgtgc taactccgac gggagcaaat ccgtgaaaga tttattaaag tttctgaaag
    10141 cgcagactaa aaccgaagag tttgatgcga tcaaaattgc tctggcttcg ccagacatga
    10201 tccgttcatg gtctttcggt gaagttaaaa agccggaaac catcaactac cgtacgttca
    10261 aaccagaacg tgacggcctt ttctgcgccc gtatctttgg gccggtaaaa gattacgagt
    10321 gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt catctgtgag aagtgcggcg
    10381 ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg ccacatcgaa ctggcttccc
    10441 cgactgcgca catctggttc ctgaaatcgc tgccgtcccg tatcggtctg ctgctcgata
    10501 tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc ctatgtggtt atcgaaggcg
    10561 gtatgaccaa cctggaacgt cagcagatcc tgactgaaga gcagtatctg gacgcgctgg
    10621 aagagttcgg tgacgaattc gacgcgaaga tgggggcgga agcaatccag gctctgctga
    10681 agagcatgga tctggagcaa gagtgcgaac agctgcgtga agagctgaac gaaaccaact
    10741 ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact gctggaagcg ttcgttcagt
    10801 ctggtaacaa accagagtgg atgatcctga ccgttctgcc ggtactgccg ccagatctgc
    10861 gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc tgacctgaac gatctgtatc
    10921 gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct ggatctggct gcgccggaca
    10981 tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt agacgccctg ctggataacg
    11041 gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc tctgaaatct ttggccgaca
    11101 tgatcaaagg taaacagggt cgtttccgtc agaacctgct cggtaagcgt gttgactact
    11161 ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct gcatcagtgc ggtctgccga
    11221 agaaaatggc actggagctg ttcaaaccgt tcatctacgg caagctggaa ctgcgtggtc
    11281 ttgctaccac cattaaagct gcgaagaaaa tggttgagcg cgaagaagct gtcgtttggg
    11341 atatcctgga cgaagttatc cgcgaacacc cggtactgct gaaccgtgca ccgactctgc
    11401 accgtctggg tatccaggca tttgaaccgg tactgatcga aggtaaagct atccagctgc
    11461 acccgctggt ttgtgcggca tataacgccg acttcgatgg tgaccagatg gctgttcacg
    11521 taccgctgac gctggaagcc cagctggaag cgcgtgcgct gatgatgtct accaacaaca
    11581 tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc tcaggacgtt gtactgggtc
    11641 tgtactacat gacccgtgac tgtgttaacg ccaaaggcga aggcatggtg ctgactggcc
    11701 cgaaagaagc agaacgtctg tatcgctctg gtctggcttc tctgcatgcg cgcgttaaag
    11761 tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt agtagcgaaa accagcctga
    11821 aagacacgac tgttggccgt gccattctgt ggatgattgt accgaaaggt ctgccttact
    11881 ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa aatgctgaac acctgctacc
    11941 gcattctcgg tctgaaaccg accgttattt ttgcggacca gatcatgtac accggcttcg
    12001 cctatgcagc gcgttctggt gcatctgttg gtatcgatga catggtcatc ccggagaaga
    12061 aacacgaaat catctccgag gcagaagcag aagttgctga aattcaggag cagttccagt
    12121 ctggtctggt aactgcgggc gaacgctaca acaaagttat cgatatctgg gctgcggcga
    12181 acgatcgtgt atccaaagcg atgatggata acctgcaaac tgaaaccgtg attaaccgtg
    12241 acggtcagga agagaagcag gtttccttca acagcatcta catgatggcc gactccggtg
    12301 cgcgtggttc tgcggcacag attcgtcagc ttgctggtat gcgtggtctg atggcgaagc
    12361 cggatggctc catcatcgaa acgccaatca ccgcgaactt ccgtgaaggt ctgaacgtac
    12421 tccagtactt catctccacc cacggtgctc gtaaaggtct ggcggatacc gcactgaaaa
    12481 ctgcgaactc cggttacctg actcgtcgtc tggttgacgt ggcgcaggac ctggtggtta
    12541 ccgaagacga ttgtggtacc catgaaggta tcatgatgac tccggttatc gagggtggtg
    12601 acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt aactgctgaa gacgttctga
    12661 agccgggtac tgctgatatc ctcgttccgc gcaacacgct gctgcacgaa cagtggtgtg
    12721 acctgctgga agagaactct gtcgacgcgg ttaaagtacg ttctgttgta tcttgtgaca
    12781 ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct ggcgcgtggc cacatcatca
    12841 acaagggtga agcaatcggt gttatcgcgg cacagtccat cggtgaaccg ggtacacagc
    12901 tgaccatgcg tacgttccac atcggtggtg cggcatctcg tgcggctgct gaatccagca
    12961 tccaagtgaa aaacaaaggt agcatcaagc tcagcaacgt gaagtcggtt gtgaactcca
    13021 gcggtaaact ggttatcact tcccgtaata ctgaactgaa actgatcgac gaattcggtc
    13081 gtactaaaga aagctacaaa gtaccttacg gtgcggtact ggcgaaaggc gatggcgaac
    13141 aggttgctgg cggcgaaacc gttgcaaact gggacccgca caccatgccg gttatcaccg
    13201 aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg ccagaccatt acgcgtcaga
    13261 ccgacgaact gaccggtctg tcttcgctgg tggttctgga ttccgcagaa cgtaccgcag
    13321 gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc tcagggtaac gacgttctga
    13381 tcccaggtac cgatatgcca gcgcagtact tcctgccggg taaagcgatt gttcagctgg
    13441 aagatggcgt acagatcagc tctggtgaca ccctggcgcg tattccgcag gaatccggcg
    13501 gtaccaagga catcaccggt ggtctgccgc gcgttgcgga cctgttcgaa gcacgtcgtc
    13561 cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt ttccttcggt aaagaaacca
    13621 aaggtaaacg tcgtctggtt atcaccccgg tagacggtag cgatccgtac gaagagatga
    13681 ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg tgtagaacgt ggtgacgtaa
    13741 tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct gcgtggtgtt catgctgtta
    13801 ctcgttacat cgttaacgaa gtacaggacg tataccgtct gcagggcgtt aagattaacg
    13861 ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa agctaccatc gttaacgcgg
    13921 gtagctccga cttcctggaa ggcgaacagg ttgaatactc tcgcgtcaag atcgcaaacc
    13981 gcgaactgga agcgaacggc aaagtgggtg caacttactc ccgcgatctg ctgggtatca
    14041 ccaaagcgtc tctggcaacc gagtccttca tctccgcggc atcgttccag gagaccactc
    14101 gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga actgcgcggc ctgaaagaga
    14161 acgttatcgt gggtcgtctg atcccggcag gtaccggtta cgcgtaccac caggatcgta
    14221 tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca ggtgactgca gaagacgcat
    14281 ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg ttctgataac gagctcgagc
    14341 tggaagttct gttccagggg cccagcagcg gccatcacca tcaccatcat caccatcacc
    14401 attaaaagct tattttgagt ggactattga ggccagagat ctttctctgt tgggttccca
    14461 tgttcgtaaa gctacctacc agacttacgc tccaattctt tatgagaatg accacttttt
    14521 cgactacatg caaaaaagta agtttcatct caccattgaa ggtccaaaag tacttgctta
    14581 tttacttggt ttatggattg gtgatggatt gtctgacagg gcaacttttt cggttgattc
    14641 cagagatact tctttgatgg aacgtgttac tgaatatgct gaaaagttga atttgtgcgc
    14701 cgagtataag gacagaaaag aaccacaagt tgccaaaact gttaatttgt actctaaagt
    14761 tgtcagaggt aatggtattc gcaataatct taatactgag aatccattat gggacgctat
    14821 tgttggctta ggattcttga aggacggtgt caaaaatatt ccttctttct tgtctacgga
    14881 caatatcggt actcgtgaaa catttcttgc tggtctaatt gattctgatg gctatgttac
    14941 tgatgagcat ggtattaaag caacaataaa gacaattcat acttctgtca gagatggttt
    15001 ggtttccctt gctcgttctt taggcttagt agtctcggtt aacgcagaac ctgctaaggt
    15061 tgacatgaat gtcaccaaac ataaaattag ttatgctatt tatatgtctg gtggagatgt
    15121 tttgcttaac gttctttcga agtgtgccgg ctctaaaaaa ttcaggcctg ctcccgccgc
    15181 tgcttttgca cgtgagtgcc gcggatttta tttcgagtta caagaattga aggaagacga
    15241 ttattatggg attactttat ctgatgattc tgatcatcag tttttgcttg gatcccaggt
    15301 tgtcgtccat gcatgcggtg gcctgaccgg tctgaactca ggcctcacga caaatcctgg
    15361 tgtatccgct tggcaggtca acacagctta tactgcggga caattggtca catataacgg
    15421 caagacgtat aaatgtttgc agccccacac ctccttggca ggatgggaac catccaacgt
    15481 tcctgccttg tggcagcttc aatgactgca gacttaagaa ggagattaat gtatggcacg
    15541 cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg tactggtcgc
    15601 cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac cggaagaaaa
    15661 cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca acaaccagat
    15721 cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat tacaagccgt
    15781 taccgctatt gctgaaggtc gtcgttaatc cggat
//
