LOCUS       sequence-254599-       15760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET21a derivative encoding E. coli core RNA polymerase subunits: 
            RpoAppx234-241-(His)10, B, C, Z.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15760)
  AUTHORS   Twist KA, Husnain SI, Franke JD, Jain D, Campbell EA, Nickels BE, 
            Thomas MS, Darst SA, Westblade LF
  TITLE     A novel method for the production of in vivo-assembled, recombinant 
            Escherichia coli RNA polymerase lacking the alpha C-terminal domain.
  JOURNAL   Protein Sci. 2011 Jun;20(6):986-95. doi: 10.1002/pro.622. Epub 2011 
            Apr 26.
  PUBMED    21416542
REFERENCE   2  (bases 1 to 15760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.622"; 
            journalName: "Protein Sci"; date: "2011-06"; volume: "20"; issue: 
            "6"; pages: "986-95"
COMMENT     Sequence Label: pEcRNAP5
FEATURES             Location/Qualifiers
     source          1..15760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(140..208,209..1000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(358..377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1042..1555)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1237..1258)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1449..1468
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1666..2254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2155..2174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2408..2425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2447..2466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2607..2629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2623..2814)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3371..4453)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4415..4434
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4454..4531)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4737..4756
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4840..4859
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4840..4858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4859..4883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4898..4920
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5627..5650
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5921..5950
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     14335..14351
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14335..14351
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             15171..15182
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             15292..15447
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             15478..15753
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
ORIGIN
        1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
       61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      301 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct
      361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
      421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
      961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag
     1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
     1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2401 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg
     2461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2641 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
     2701 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     2761 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     2821 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     2881 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     2941 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc
     3001 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     3061 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     3121 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     3181 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     3241 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     3301 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
     3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
     3481 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
     3541 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
     3601 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
     3661 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
     3721 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
     3781 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
     3841 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
     3901 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     3961 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     4021 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     4081 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     4141 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     4201 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     4261 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     4321 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     4381 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     4441 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     4501 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
     4561 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     4621 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     4681 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     4741 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     4801 cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg
     4861 aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga
     4921 tatacatatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg atatcgagca
     4981 agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct ttggccatac
     5041 tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg cggtgaccga
     5101 ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc aggaagatat
     5161 cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca aagatgaagt
     5221 tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata tcacccacga
     5281 cggtgatgtc gaaatcgtca agccgcagca cgtgatctgc cacctgaccg atgagaacgc
     5341 gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg cttctacccg
     5401 aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg catgctacag
     5461 ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc gtaccgacct
     5521 ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag aggcgattcg
     5581 tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttag aagttttatt
     5641 tcagggtcca gtgaaagaag agaaaccaga gttcgatccg atcctgctgc gccctgttga
     5701 cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta tccactatat
     5761 cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa
     5821 atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct
     5881 ggaaaactgg ccaccggcaa gcatcgctga cgaggctagc catcaccatc accatcatca
     5941 ccatcaccat taaccatgga tccccgatcc gtcgacttgt cagcgagctg aggaacccta
     6001 tggtttactc ctataccgag aaaaaacgta ttcgtaagga ttttggtaaa cgtccacaag
     6061 ttctggatgt accttatctc ctttctatcc agcttgactc gtttcagaaa tttatcgagc
     6121 aagatcctga agggcagtat ggtctggaag ctgctttccg ttccgtattc ccgattcaga
     6181 gctacagcgg taattccgag ctgcaatacg tcagctaccg ccttggcgaa ccggtgtttg
     6241 acgtccagga atgtcaaatc cgtggcgtga cctattccgc accgctgcgc gttaaactgc
     6301 gtctggtgat ctatgagcgc gaagcgccgg aaggcaccgt aaaagacatt aaagaacaag
     6361 aagtctacat gggcgaaatt ccgctcatga cagacaacgg tacctttgtt atcaacggta
     6421 ctgagcgtgt tatcgtttcc cagctgcacc gtagtccggg cgtcttcttt gactccgaca
     6481 aaggtaaaac ccactcttcg ggtaaagtgc tgtataacgc gcgtatcatc ccttaccgtg
     6541 gttcctggct ggacttcgaa ttcgatccga aggacaacct gttcgtacgt atcgaccgtc
     6601 gccgtaaact gcctgcgacc atcattctgc gcgccctgaa ctacaccaca gagcagatcc
     6661 tcgacctgtt ctttgaaaaa gttatctttg aaatccgtga taacaagctg cagatggaac
     6721 tggtgccgga acgcctgcgt ggtgaaaccg catcttttga catcgaagct aacggtaaag
     6781 tgtacgtaga aaaaggccgc cgtatcactg cgcgccacat tcgccagctg gaaaaagacg
     6841 acgtcaaact gatcgaagtc ccggttgagt acatcgcagg taaagtggtt gctaaagact
     6901 atattgatga gtctaccggc gagctgatct gcgcagcgaa catggagctg agcctggatc
     6961 tgctggctaa gctgagccag tctggtcaca agcgtatcga aacgctgttc accaacgatc
     7021 tggatcacgg cccatatatc tctgaaacct tacgtgtcga cccaactaac gaccgtctga
     7081 gcgcactggt agaaatctac cgcatgatgc gccctggcga gccgccgact cgtgaagcag
     7141 ctgaaagcct gttcgagaac ctgttcttct ccgaagaccg ttatgacttg tctgcggttg
     7201 gtcgtatgaa gttcaaccgt tctctgctgc gcgaagaaat cgaaggttcc ggtatcctga
     7261 gcaaagacga catcattgat gttatgaaaa agctcatcga tatccgtaac ggtaaaggcg
     7321 aagtcgatga tatcgaccac ctcggcaacc gtcgtatccg ttccgttggc gaaatggcgg
     7381 aaaaccagtt ccgcgttggc ctggtacgtg tagagcgtgc ggtgaaagag cgtctgtctc
     7441 tgggcgatct ggataccctg atgccacagg atatgatcaa cgccaagccg atttccgcag
     7501 cagtgaaaga gttcttcggt tccagccagc tgtctcagtt tatggaccag aacaacccgc
     7561 tgtctgagat tacgcacaaa cgtcgtatct ccgcactcgg cccaggcggt ctgacccgtg
     7621 aacgtgcagg cttcgaagtt cgagacgtac acccgactca ctacggtcgc gtatgtccaa
     7681 tcgaaacccc tgaaggtccg aacatcggtc tgatcaactc tctgtccgtg tacgcacaga
     7741 ctaacgaata cggcttcctt gagactccgt atcgtaaagt gaccgacggt gttgtaactg
     7801 acgaaattca ctacctgtct gctatcgaag aaggcaacta cgttatcgcc caggcgaact
     7861 ccaacttgga tgaagaaggc cacttcgtag aagacctggt aacttgccgt agcaaaggcg
     7921 aatccagctt gttcagccgc gaccaggttg actacatgga cgtatccacc cagcaggtgg
     7981 tatccgtcgg tgcgtccctg atcccgttcc tggaacacga tgacgccaac cgtgcattga
     8041 tgggtgcgaa catgcaacgt caggccgttc cgactctgcg cgctgataag ccgctggttg
     8101 gtactggtat ggaacgtgct gttgccgttg actccggtgt aactgcggta gctaaacgtg
     8161 gtggtgtcgt tcagtacgtg gatgcttccc gtatcgttat caaagttaac gaagacgaga
     8221 tgtatccggg tgaagcaggt atcgacatct acaacctgac caaatacacc cgttctaacc
     8281 agaacacctg tatcaaccag atgccgtgtg tgtctctggg tgaaccggtt gaacgtggcg
     8341 acgtgctggc agacggtccg tccaccgacc tcggtgaact ggcgcttggt cagaacatgc
     8401 gcgtagcgtt catgccgtgg aatggttaca acttcgaaga ctccatcctc gtatccgagc
     8461 gtgttgttca ggaagaccgt ttcaccacca tccacattca ggaactggcg tgtgtgtccc
     8521 gtgacaccaa gctgggtccg gaagagatca ccgctgacat cccgaacgtg ggtgaagctg
     8581 cgctctccaa actggatgaa tccggtatcg tttacattgg tgcggaagtg accggtggcg
     8641 acattctggt tggtaaggta acgccgaaag gtgaaactca gctgacccca gaagaaaaac
     8701 tgctgcgtgc gatcttcggt gagaaagcct ctgacgttaa agactcttct ctgcgcgtac
     8761 caaacggtgt atccggtacg gttatcgacg ttcaggtctt tactcgcgat ggcgtagaaa
     8821 aagacaaacg tgcgctggaa atcgaagaaa tgcagctcaa acaggcgaag aaagacctgt
     8881 ctgaagaact gcagatcctc gaagcgggtc tgttcagccg tatccgtgct gtgctggtag
     8941 ccggtggcgt tgaagctgag aagctcgaca aactgccgcg cgatcgctgg ctggagctgg
     9001 gcctgacaga cgaagagaaa caaaatcagc tggaacagct ggctgagcag tatgacgaac
     9061 tgaaacacga gttcgagaag aaactcgaag cgaaacgccg caaaatcacc cagggcgacg
     9121 atctggcacc gggcgtgctg aagattgtta aggtatatct ggcggttaaa cgccgtatcc
     9181 agcctggtga caagatggca ggtcgtcacg gtaacaaggg tgtaatttct aagatcaacc
     9241 cgatcgaaga tatgccttac gatgaaaacg gtacgccggt agacatcgta ctgaacccgc
     9301 tgggcgtacc gtctcgtatg aacatcggtc agatcctcga aacccacctg ggtatggctg
     9361 cgaaaggtat cggcgacaag atcaacgcca tgctgaaaca gcagcaagaa gtcgcgaaac
     9421 tgcgcgaatt catccagcgt gcgtacgatc tgggcgctga cgttcgtcag aaagttgacc
     9481 tgagtacctt cagcgatgaa gaagttatgc gtctggctga aaacctgcgc aaaggtatgc
     9541 caatcgcaac gccggtgttc gacggtgcga aagaagcaga aattaaagag ctgctgaaac
     9601 ttggcgacct gccgacttcc ggtcagatcc gcctgtacga tggtcgcact ggtgaacagt
     9661 tcgagcgtcc ggtaaccgtt ggttacatgt acatgctgaa actgaaccac ctggtcgacg
     9721 acaagatgca cgcgcgttcc accggttctt acagcctggt tactcagcag ccgctgggtg
     9781 gtaaggcaca gttcggtggt cagcgtttcg gggagatgga agtgtgggcg ctggaagcat
     9841 acggcgcagc atacaccctg caggaaatgc tcaccgttaa gtctgatgac gtgaacggtc
     9901 gtaccaagat gtataaaaac atcgtggacg gcaaccatca gatggagccg ggcatgccag
     9961 aatccttcaa cgtattgttg aaagagattc gttcgctggg tatcaacatc gaactggaag
    10021 acgagtaatt ctcgctcaaa caggtcactg ctgtcgggtt aaaacccggc agcggattgt
    10081 gctaactccg acgggagcaa atccgtgaaa gatttattaa agtttctgaa agcgcagact
    10141 aaaaccgaag agtttgatgc gatcaaaatt gctctggctt cgccagacat gatccgttca
    10201 tggtctttcg gtgaagttaa aaagccggaa accatcaact accgtacgtt caaaccagaa
    10261 cgtgacggcc ttttctgcgc ccgtatcttt gggccggtaa aagattacga gtgcctgtgc
    10321 ggtaagtaca agcgcctgaa acaccgtggc gtcatctgtg agaagtgcgg cgttgaagtg
    10381 acccagacta aagtacgccg tgagcgtatg ggccacatcg aactggcttc cccgactgcg
    10441 cacatctggt tcctgaaatc gctgccgtcc cgtatcggtc tgctgctcga tatgccgctg
    10501 cgcgatatcg aacgcgtact gtactttgaa tcctatgtgg ttatcgaagg cggtatgacc
    10561 aacctggaac gtcagcagat cctgactgaa gagcagtatc tggacgcgct ggaagagttc
    10621 ggtgacgaat tcgacgcgaa gatgggggcg gaagcaatcc aggctctgct gaagagcatg
    10681 gatctggagc aagagtgcga acagctgcgt gaagagctga acgaaaccaa ctccgaaacc
    10741 aagcgtaaaa agctgaccaa gcgtatcaaa ctgctggaag cgttcgttca gtctggtaac
    10801 aaaccagagt ggatgatcct gaccgttctg ccggtactgc cgccagatct gcgtccgctg
    10861 gttccgctgg atggtggtcg tttcgcgact tctgacctga acgatctgta tcgtcgcgtc
    10921 attaaccgta acaaccgtct gaaacgtctg ctggatctgg ctgcgccgga catcatcgta
    10981 cgtaacgaaa aacgtatgct gcaggaagcg gtagacgccc tgctggataa cggtcgtcgc
    11041 ggtcgtgcga tcaccggttc taacaagcgt cctctgaaat ctttggccga catgatcaaa
    11101 ggtaaacagg gtcgtttccg tcagaacctg ctcggtaagc gtgttgacta ctccggtcgt
    11161 tctgtaatca ccgtaggtcc atacctgcgt ctgcatcagt gcggtctgcc gaagaaaatg
    11221 gcactggagc tgttcaaacc gttcatctac ggcaagctgg aactgcgtgg tcttgctacc
    11281 accattaaag ctgcgaagaa aatggttgag cgcgaagaag ctgtcgtttg ggatatcctg
    11341 gacgaagtta tccgcgaaca cccggtactg ctgaaccgtg caccgactct gcaccgtctg
    11401 ggtatccagg catttgaacc ggtactgatc gaaggtaaag ctatccagct gcacccgctg
    11461 gtttgtgcgg catataacgc cgacttcgat ggtgaccaga tggctgttca cgtaccgctg
    11521 acgctggaag cccagctgga agcgcgtgcg ctgatgatgt ctaccaacaa catcctgtcc
    11581 ccggcgaacg gcgaaccaat catcgttccg tctcaggacg ttgtactggg tctgtactac
    11641 atgacccgtg actgtgttaa cgccaaaggc gaaggcatgg tgctgactgg cccgaaagaa
    11701 gcagaacgtc tgtatcgctc tggtctggct tctctgcatg cgcgcgttaa agtgcgtatc
    11761 accgagtatg aaaaagatgc taacggtgaa ttagtagcga aaaccagcct gaaagacacg
    11821 actgttggcc gtgccattct gtggatgatt gtaccgaaag gtctgcctta ctccatcgtc
    11881 aaccaggcgc tgggtaaaaa agcaatctcc aaaatgctga acacctgcta ccgcattctc
    11941 ggtctgaaac cgaccgttat ttttgcggac cagatcatgt acaccggctt cgcctatgca
    12001 gcgcgttctg gtgcatctgt tggtatcgat gacatggtca tcccggagaa gaaacacgaa
    12061 atcatctccg aggcagaagc agaagttgct gaaattcagg agcagttcca gtctggtctg
    12121 gtaactgcgg gcgaacgcta caacaaagtt atcgatatct gggctgcggc gaacgatcgt
    12181 gtatccaaag cgatgatgga taacctgcaa actgaaaccg tgattaaccg tgacggtcag
    12241 gaagagaagc aggtttcctt caacagcatc tacatgatgg ccgactccgg tgcgcgtggt
    12301 tctgcggcac agattcgtca gcttgctggt atgcgtggtc tgatggcgaa gccggatggc
    12361 tccatcatcg aaacgccaat caccgcgaac ttccgtgaag gtctgaacgt actccagtac
    12421 ttcatctcca cccacggtgc tcgtaaaggt ctggcggata ccgcactgaa aactgcgaac
    12481 tccggttacc tgactcgtcg tctggttgac gtggcgcagg acctggtggt taccgaagac
    12541 gattgtggta cccatgaagg tatcatgatg actccggtta tcgagggtgg tgacgttaaa
    12601 gagccgctgc gcgatcgcgt actgggtcgt gtaactgctg aagacgttct gaagccgggt
    12661 actgctgata tcctcgttcc gcgcaacacg ctgctgcacg aacagtggtg tgacctgctg
    12721 gaagagaact ctgtcgacgc ggttaaagta cgttctgttg tatcttgtga caccgacttt
    12781 ggtgtatgtg cgcactgcta cggtcgtgac ctggcgcgtg gccacatcat caacaagggt
    12841 gaagcaatcg gtgttatcgc ggcacagtcc atcggtgaac cgggtacaca gctgaccatg
    12901 cgtacgttcc acatcggtgg tgcggcatct cgtgcggctg ctgaatccag catccaagtg
    12961 aaaaacaaag gtagcatcaa gctcagcaac gtgaagtcgg ttgtgaactc cagcggtaaa
    13021 ctggttatca cttcccgtaa tactgaactg aaactgatcg acgaattcgg tcgtactaaa
    13081 gaaagctaca aagtacctta cggtgcggta ctggcgaaag gcgatggcga acaggttgct
    13141 ggcggcgaaa ccgttgcaaa ctgggacccg cacaccatgc cggttatcac cgaagtaagc
    13201 ggttttgtac gctttactga catgatcgac ggccagacca ttacgcgtca gaccgacgaa
    13261 ctgaccggtc tgtcttcgct ggtggttctg gattccgcag aacgtaccgc aggtggtaaa
    13321 gatctgcgtc cggcactgaa aatcgttgat gctcagggta acgacgttct gatcccaggt
    13381 accgatatgc cagcgcagta cttcctgccg ggtaaagcga ttgttcagct ggaagatggc
    13441 gtacagatca gctctggtga caccctggcg cgtattccgc aggaatccgg cggtaccaag
    13501 gacatcaccg gtggtctgcc gcgcgttgcg gacctgttcg aagcacgtcg tccgaaagag
    13561 ccggcaatcc tggctgaaat cagcggtatc gtttccttcg gtaaagaaac caaaggtaaa
    13621 cgtcgtctgg ttatcacccc ggtagacggt agcgatccgt acgaagagat gattccgaaa
    13681 tggcgtcagc tcaacgtgtt cgaaggtgaa cgtgtagaac gtggtgacgt aatttccgac
    13741 ggtccggaag cgccgcacga cattctgcgt ctgcgtggtg ttcatgctgt tactcgttac
    13801 atcgttaacg aagtacagga cgtataccgt ctgcagggcg ttaagattaa cgataaacac
    13861 atcgaagtta tcgttcgtca gatgctgcgt aaagctacca tcgttaacgc gggtagctcc
    13921 gacttcctgg aaggcgaaca ggttgaatac tctcgcgtca agatcgcaaa ccgcgaactg
    13981 gaagcgaacg gcaaagtggg tgcaacttac tcccgcgatc tgctgggtat caccaaagcg
    14041 tctctggcaa ccgagtcctt catctccgcg gcatcgttcc aggagaccac tcgcgtgctg
    14101 accgaagcag ccgttgcggg caaacgcgac gaactgcgcg gcctgaaaga gaacgttatc
    14161 gtgggtcgtc tgatcccggc aggtaccggt tacgcgtacc accaggatcg tatgcgtcgc
    14221 cgtgctgcgg gtgaagctcc ggctgcaccg caggtgactg cagaagacgc atctgccagc
    14281 ctggcagaac tgctgaacgc aggtctgggc ggttctgata acgagctcga gtgacaggaa
    14341 acagctatga cagcttattt tgagtggact attgaggcca gagatctttc tctgttgggt
    14401 tcccatgttc gtaaagctac ctaccagact tacgctccaa ttctttatga gaatgaccac
    14461 tttttcgact acatgcaaaa aagtaagttt catctcacca ttgaaggtcc aaaagtactt
    14521 gcttatttac ttggtttatg gattggtgat ggattgtctg acagggcaac tttttcggtt
    14581 gattccagag atacttcttt gatggaacgt gttactgaat atgctgaaaa gttgaatttg
    14641 tgcgccgagt ataaggacag aaaagaacca caagttgcca aaactgttaa tttgtactct
    14701 aaagttgtca gaggtaatgg tattcgcaat aatcttaata ctgagaatcc attatgggac
    14761 gctattgttg gcttaggatt cttgaaggac ggtgtcaaaa atattccttc tttcttgtct
    14821 acggacaata tcggtactcg tgaaacattt cttgctggtc taattgattc tgatggctat
    14881 gttactgatg agcatggtat taaagcaaca ataaagacaa ttcatacttc tgtcagagat
    14941 ggtttggttt cccttgctcg ttctttaggc ttagtagtct cggttaacgc agaacctgct
    15001 aaggttgaca tgaatgtcac caaacataaa attagttatg ctatttatat gtctggtgga
    15061 gatgttttgc ttaacgttct ttcgaagtgt gccggctcta aaaaattcag gcctgctccc
    15121 gccgctgctt ttgcacgtga gtgccgcgga ttttatttcg agttacaaga attgaaggaa
    15181 gacgattatt atgggattac tttatctgat gattctgatc atcagttttt gcttggatcc
    15241 caggttgtcg tccatgcatg cggtggcctg accggtctga actcaggcct cacgacaaat
    15301 cctggtgtat ccgcttggca ggtcaacaca gcttatactg cgggacaatt ggtcacatat
    15361 aacggcaaga cgtataaatg tttgcagccc cacacctcct tggcaggatg ggaaccatcc
    15421 aacgttcctg ccttgtggca gcttcaatga ctgcagactt aagaaggaga ttaatgtatg
    15481 gcacgcgtaa ctgttcagga cgctgtagag aaaattggta accgttttga cctggtactg
    15541 gtcgccgcgc gtcgcgctcg tcagatgcag gtaggcggaa aggatccgct ggtaccggaa
    15601 gaaaacgata aaaccactgt aatcgcgctg cgcgaaatcg aagaaggtct gatcaacaac
    15661 cagatcctcg acgttcgcga acgccaggaa cagcaagagc aggaagccgc tgaattacaa
    15721 gccgttaccg ctattgctga aggtcgtcgt taatccggat
//
