LOCUS       sequence-254600-        9311 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Chen L, Cai Y, Jin J, Florens L, Swanson SK, Washburn MP, Conaway 
            JW, Conaway RC
  TITLE     Subunit organization of the human INO80 chromatin remodeling 
            complex: an evolutionarily conserved core complex catalyzes 
            ATP-dependent nucleosome remodeling.
  JOURNAL   J Biol Chem. 2011 Apr 1;286(13):11283-9. doi: 
            10.1074/jbc.M111.222505. Epub 2011 Feb 8.
  PUBMED    21303910
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.222505"; journalName: "J Biol Chem"; date: 
            "2011-04-1- 1"; volume: "286"; issue: "13"; pages: "11283-9"
COMMENT     Sequence Label: pCMV-3XFLAG-hINO80
FEATURES             Location/Qualifiers
     source          1..9311
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     582..604
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     596..613
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     597..613
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        774..1153
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1154..1357
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1308..1328
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1387..1452
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5223..5234
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    6180..6802
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(6379..6398)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6826..6846)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6870..7080
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        6892..7088
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6939..7074
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7126..7145)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7127..7145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7159..7175)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7159..7175)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7172..7194)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7183..7199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7207..7213,7214..7231,7232..7237))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7252..7273
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7390..7407)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7561..8149)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7641..7660)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8320..9111,9112..9180))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8943..8962
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9181..9285)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa
      481 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      541 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      601 aacgacggcc agtgccaagc tgatctatac attgaatcaa tattggcaat tagccatatt
      661 agtcattggt tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct
      721 atatcataat atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg
      781 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      841 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      901 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      961 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     1021 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     1081 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     1141 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     1201 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     1261 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     1321 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagaatta
     1381 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat
     1441 gacgatgaca agcttgcggc cgcgaattca atggcctcgg agttgggtgc cagggatgat
     1501 ggaggctgca ctgagctggc aaagcccctc tatcttcagt acttggagag ggccctccgg
     1561 ttggaccatt ttctgcgaca aacgtcagct atcttcaata ggaatatttc tagtgatgac
     1621 agtgaagatg gactggatga cagtaatcca ttattgcccc agtctgggga tcccttaata
     1681 caagttaagg aagaacctcc aaattcattg cttggtgaaa cttctggagc aggcagttct
     1741 ggaatgttaa acacatattc tctgaatgga gttctacagt cagaatcaaa atgtgataag
     1801 gggaatttat ataatttctc taagctgaag aaaagcagaa agtggctaaa gagcattctg
     1861 ctaagtgatg aatccagcga ggctgattct cagagtgaag acgatgatga agaagaactc
     1921 aatctcagca gagaagaact tcacaacatg cttcgactac acaaatataa gaaacttcac
     1981 caaaataagt atagtaaaga caaggagttg cagcaatatc agtactacag tgcaggcctg
     2041 ctctccacat atgacccttt ctatgagcaa caacggcacc tacttggacc caagaaaaag
     2101 aaatttaagg aggaaaagaa acttaaagct aagttgaaaa aagtgaagaa aaaaagacga
     2161 agagatgaag aactttcctc tgaagaatcc cctcgtcgcc atcaccacca gaccaaagtc
     2221 tttgccaagt tttctcacga tgcacctccc cctggcacta agaaaaagca cttatccatt
     2281 gagcagctga atgctcgtcg caggaaagta tggctcagca ttgtgaaaaa ggaactacca
     2341 aaggcaaata agcagaaagc ttcagctcgt aacctgtttc tcaccaatag ccgaaagctt
     2401 gctcaccagt gcatgaagga ggtgcgtcga gctgccttgc aggcccagaa gaactgtaag
     2461 gaaaccttgc ctcgtgcccg ccgcctcacc aaggagatgc ttctgtactg gaagaaatat
     2521 gagaaagtag agaaggagca ccgcaagaga gcagagaagg aagctttgga gcagcggaag
     2581 ttggatgagg aaatgcggga ggccaagagg caacagcgaa aactcaactt cttaattacc
     2641 cagacagagt tgtatgccca tttcatgagt cgcaaacgag atatgggtca tgatggtatc
     2701 caggaagaaa tcctaaggaa actggaagac agttctaccc agagacaaat cgatataggt
     2761 ggaggagtgg tagttaacat cacacaggag gattatgata gtaaccattt taaagcccag
     2821 gccctgaaga atgctgaaaa tgcttaccat attcaccaag ctcggacaag gtcatttgat
     2881 gaagatgcaa aagaaagtcg agcagctgcc ctacgggcag caaacaagtc tggcactggg
     2941 tttggggaga gttatagcct ggctaaccca tctatccggg ctggtgagga tattccacag
     3001 cccacaattt ttaatggcaa attgaaaggt tatcaactga aaggcatgaa ttggttggcc
     3061 aatctatatg aacagggtat taatggcatt cttgctgatg aaatgggcct tggtaaaaca
     3121 gtacagagca ttgcccttct ggcccatctg gctgagagag agaacatttg gggacctttc
     3181 ttaataattt cacctgcgtc tacacttaac aattggcacc aggagtttac tagatttgtt
     3241 cctaaattta aggtgctacc atattgggga aatcctcatg atagaaaagt catcagaagg
     3301 ttctggagtc agaagaccct ctacactcag gatgccccct tccatgtggt tattaccagc
     3361 tatcagctgg tggtgcagga tgtaaagtat ttccagcggg tcaagtggca atacatggta
     3421 ctggatgagg ctcaggcgct caagagtagt tccagtgttc gttggaagat cctcttacag
     3481 ttccagtgtc ggaatcggct tttgctaacc gggaccccaa ttcagaacac catggcagag
     3541 ctttgggctc tgctgcattt cattatgcca acattatttg attcacatga ggaatttaat
     3601 gaatggtttt ccaaggacat tgagagccat gccgaaaaca aatctgctat tgatgagaat
     3661 caactttctc gcttacacat gattttgaag ccatttatgc tgaggagaat caagaaagat
     3721 gtggaaaatg aattatctga caagattgag attctaatgt attgccaact gaccagccga
     3781 cagaagctgc tatatcaggc actaaagaac aaaatttcca ttgaggattt attgcagtct
     3841 tctatgggct ctacccaaca agcacagaac accaccagca gcctcatgaa tctggtcatg
     3901 cagtttagga aggtgtgtaa tcacccggag ttatttgaac ggcaagaaac ttggtctcca
     3961 tttcatattt ccctaaagcc ataccacatt tcaaagttta tctaccgtca tggacagatc
     4021 agggtcttca atcattcacg agacaggtgg ttaagggttc tttctccatt tgcaccagac
     4081 tatatccaac ggtctctctt tcacagaaaa ggtattaatg aagaaagctg tttctctttc
     4141 cttcgcttta ttgatatatc tccagcagaa atggcaaacc ttatgcttca gggacttttg
     4201 gccagatggt tagctctttt cctgtctctg aaagcctcct acaggctcca tcagctacgc
     4261 tcctggggag cgccagaagg ggagagccac cagagatacc tgaggaacaa ggatttcctt
     4321 cttggggtta attttccact ctcctttcca aacctttgca gctgcccttt gttaaagtct
     4381 cttgttttca gcagccactg taaagcagtg agtggctact cagaccaggt tgtccatcag
     4441 cggagatcag ctacctcctc gctgcgtcgc tgcctgctca ctgagctgcc atcttttttg
     4501 tgtgtggcca gtccacgagt taccgcagtg ccattggatt cttactgcaa tgaccgaagt
     4561 gcagaatatg aaaggcgagt tctgaaggaa ggagggagtc tggcagccaa gcagtgtttg
     4621 ttgaatgggg cccctgaact ggctgcagac tggctaaata gacgatcaca gttcttccca
     4681 gagccagctg gaggtctgtg gagcatcaga cctcagaatg gctggtcttt catcaggatt
     4741 ccaggcaagg agagcctcat cactgacagt ggaaagctgt atgcccttga tgtcctgctg
     4801 actcggctca agtctcaagg gcatagggtc cttatctact cccagatgac caggatgata
     4861 gacctactgg aggaatacat ggtttacagg aagcatacct acatgaggct tgatggctca
     4921 tccaagatct cggagaggcg agacatggtt gctgattttc agaacaggaa tgacatcttt
     4981 gtgttcctgt taagcacacg agctggagga ctgggtatca atctcactgc tgcagacaca
     5041 gtgattttct atgatagcga ctggaacccc actgtggacc agcaggccat ggacagggcc
     5101 caccgcttag ggcagacaaa gcaggttact gtgtaccggc tcatctgtaa aggcaccatt
     5161 gaagaacgca ttctgcaaag agccaaggag aagagtgaga ttcagcggat ggtgatttca
     5221 ggtgggaact tcaaaccaga taccttgaaa cccaaagagg tggttagtct tcttctagac
     5281 gacgaagagt tggagaagaa actgaggctg cggcaggaag agaaacggca acaggaggaa
     5341 accaaccgag tgaaagagcg caagcggaag cgggaaaagt atgcagagaa gaagaaaaaa
     5401 gaagatgaat tggatgggaa aaggagaaaa gagggtgtga acctggtgat cccatttgtt
     5461 ccctcggctg ataactccaa cctctctgct gacggagatg actccttcat tagcgtggac
     5521 tcagccatgc caagcccttt cagtgagatc tccatcagca gtgagctgca cactggctcc
     5581 attcccctgg acgagagcag cagtgacatg ctggtcattg tggatgaccc agcctcctca
     5641 gcccctcagt ctcgagctac caactctccc gcatccataa caggctccgt ctcagatacc
     5701 gtgaatggaa tttccattca ggaaatgcca gctgcaggac gtggtcactc agcccgaagc
     5761 cgaggccgcc ccaaaggttc aggaagcaca gccaaaggag cagggaaggg ccggagccga
     5821 aagtccacgg caggcagtgc tgctgcaatg gcaggagcca aagccggggc tgcagcggcc
     5881 tctgcagctg cctatgccgc atacgggtac aacgtgtcta aaggaatctc cgccagcagt
     5941 cctctgcaga catcccttgt tcggcctgct ggccttgctg actttggacc ttcaagcgcc
     6001 tcttctcctt tgagttcccc tttgagcaag ggaaataatg ttcctgggaa tcccaagaac
     6061 ctccacatga ccagcagcct agccccagac tctctggtcc ggaaacaggg caaaggcacc
     6121 aacccctctg gaggacggta accatctgat atcggtacca gtcgactcta gaggatcccg
     6181 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
     6241 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
     6301 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
     6361 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
     6421 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
     6481 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
     6541 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
     6601 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
     6661 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg
     6721 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca
     6781 ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt
     6841 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6901 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     6961 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7021 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7081 tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt taattcccta tagtgagtcg
     7141 tattaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     7201 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     7261 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     7321 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     7381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7981 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     8041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8281 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8341 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8401 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8461 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8521 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8581 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     8641 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     8701 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     8761 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     8821 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8881 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8941 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     9001 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     9061 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     9121 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     9181 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     9241 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     9301 agtgccacct g
//
