LOCUS       sequence-254603-       10595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Gateway-compatible expression vector backbone with a 
            C-terminal Firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10595)
  TITLE     Taipale Lab plasmids
REFERENCE   2  (bases 1 to 10595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti6.2-ccdB-Firefly
FEATURES             Location/Qualifiers
     source          1..10595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..145
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        207..254
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             273..671
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             758..991
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1063..1184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1100..1119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1154..1173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1224..1359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1379..1398)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1380..1398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1408..1425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1408..1424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1417..1439)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1566..2021
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1653..1672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1863..1884
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2047..2151
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2152..2220,2221..3012)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2370..2389)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3183..3771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3672..3691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3925..3942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4059..4080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4095..4100,4101..4118,4119..4125)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4133..4149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4138..4160
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4157..4173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4157..4173
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4192..4212
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4194..4212
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4240..4466
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4467..4647
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4694..4819
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5312..5545
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5730..5774
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        6119..6422
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6423..6626
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6577..6597
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6623..6647
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    6771..6895
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6920..6925,6926..6943,6944..6950)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7004..7663
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7070..7089)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8005..8310
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8189..8208
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8351..8475)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8543..10192
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
                     IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
                     NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
                     YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
                     DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
                     QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
                     LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
                     YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
                     QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
                     VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(8606..8624)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             10215..10256
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        join(10289..10595,1..193)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10324..10347)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
       61 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
      121 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
      181 tccgggcctt tcgactctag acacgtgttg acaattaatc atcggcatag tatatcggca
      241 tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat
      301 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
      361 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
      421 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
      481 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
      541 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
      601 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
      661 gggagggcta agcacaattc gagctcggta cctttaagac caatgactta caaggcagct
      721 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
      781 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
      841 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      901 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      961 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
     1021 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     1081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     1141 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     1201 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     1261 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     1321 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     1381 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     1441 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     1501 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     1561 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     1621 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     1681 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     1741 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     1801 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     1861 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     1921 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     1981 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     2041 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     2101 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     2161 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     2221 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     2281 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     2341 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     2401 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2461 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2521 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2581 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2641 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2701 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2761 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2821 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2881 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2941 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     3001 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     3061 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3301 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     3361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3961 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     4021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     4081 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     4141 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     4201 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     4261 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     4321 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     4381 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     4441 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     4501 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4561 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     4621 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     4681 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     4741 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     4801 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     4861 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     4921 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     4981 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     5041 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     5101 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     5161 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     5221 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     5281 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     5341 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     5401 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     5461 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     5521 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     5581 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     5641 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     5701 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     5761 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     5821 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     5881 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     5941 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     6001 ccattcgatt agtgaacgga tctcgacggt atcgatgtcg acgttaacgc tagcgactgg
     6061 gtctgcgatg tcgacgttaa cgctagcgac tgggtctgcg ataagcttgg gagttccgcg
     6121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     6181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     6241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     6301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     6361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     6421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     6481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     6541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     6601 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     6661 atccacgctg ttttgacctc catagaagac accgactcta gcgtttaaac ttaagcttgg
     6721 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatca acaagtttgt
     6781 acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt
     6841 tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc
     6901 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     6961 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     7021 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     7081 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     7141 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     7201 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     7261 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     7321 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     7381 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     7441 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     7501 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     7561 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     7621 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     7681 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     7741 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     7801 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     7861 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     7921 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     7981 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     8041 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     8101 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     8161 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     8221 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     8281 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     8341 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     8401 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     8461 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtggct cgggctcgac
     8521 ctcgggctcg ggcaaaaccg gtgaagacgc caaaaacata aagaaaggcc cggcgccatt
     8581 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
     8641 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
     8701 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
     8761 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
     8821 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
     8881 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
     8941 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
     9001 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
     9061 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
     9121 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
     9181 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
     9241 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
     9301 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
     9361 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
     9421 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     9481 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     9541 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     9601 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     9661 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     9721 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     9781 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     9841 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     9901 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     9961 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
    10021 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
    10081 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
    10141 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt agtctagagg
    10201 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
    10261 ccggttagta atgacgatgg tacctaccgg gtaggggagg cgcttttccc aaggcagtct
    10321 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
    10381 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
    10441 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
    10501 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
    10561 gctgagcaat ggaagcgggt aggcctttgg ggcag
//
