LOCUS       sequence-25461-e         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC225
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(148..453)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(250..269)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
       61 aaacagacta cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga
      121 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      181 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      241 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      301 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      361 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      421 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
      481 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      541 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
      601 agatattgac atcatatatg ccttgagcac
//
