LOCUS       sequence-254610-       10671 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NOTCH-ICD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Cassady JP, D'Alessio AC, Sarkar S, Dani VS, Fan ZP, Ganz K, 
            Roessler R, Sur M, Young RA, Jaenisch R
  TITLE     Direct Lineage Conversion of Adult Mouse Liver Cells and B 
            Lymphocytes to Neural Stem Cells.
  JOURNAL   Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001.
  PUBMED    25454632
REFERENCE   2  (bases 1 to 10671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. doi: 
            10.1016/j.stemcr.2014.10.001."
COMMENT     Sequence Label: TetO-FUW-NICD
FEATURES             Location/Qualifiers
     source          1..10671
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1780..1798
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1822..1840
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1864..1882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        1915..1953
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1950..1974
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4529..5117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4582..4602)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5000..5011)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5120..5136)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5121..5137)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5642..5822
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5848..5865)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5854..6078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6124..6552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6211..6230)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6421..6442
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6561..6581)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6566..6895
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6746..6881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6808..6827
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6943..6990
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7009..7383
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7513..7634
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7550..7569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7604..7623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7683..7699)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7683..7699)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7696..7718)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7707..7723
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7731..7737,7738..7755,7756..7761))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7776..7797
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7914..7931)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8085..8673)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8165..8184)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8844..9635,9636..9704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9467..9486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9705..9809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9884..9903)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10075..10454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10456..10654
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt tagatctcga gtttaccact ccctatcagt gatagagaaa
     1801 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     1861 cactccctat cagtgataga gaaaagtgaa agtcgagctc ggtacccggg tcgaggtagg
     1921 cgtgtacggt gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     1981 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc
     2041 ggccccgaat tcgcccttgc caccatgcgg cggcagcatg gccagctctg gttccctgag
     2101 ggcttcaaag tgtctgaggc cagcaagaag aagcggcggg agcccctcgg cgaggactcc
     2161 gtgggcctca agcccctgaa gaacgcttca gacggtgccc tcatggacga caaccagaat
     2221 gagtgggggg acgaggacct ggagaccaag aagttccggt tcgaggagcc cgtggttctg
     2281 cctgacctgg acgaccagac agaccaccgg cagtggactc agcagcacct ggatgccgct
     2341 gacctgcgca tgtctgccat ggcccccaca ccgccccagg gtgaggttga cgccgactgc
     2401 atggacgtca atgtccgcgg gcctgatggc ttcaccccgc tcatgatcgc ctcctgcagc
     2461 gggggcggcc tggagacggg caacagcgag gaagaggagg acgcgccggc cgtcatctcc
     2521 gacttcatct accagggcgc cagcctgcac aaccagacag accgcacggg cgagaccgcc
     2581 ttgcacctgg ccgcccgcta ctcacgctct gattccgcca agcgcctgct ggaggccagc
     2641 gcagatgcca acatccagga caacatgggc cgcaccccgc tgcatgcggc tgtgtctgcc
     2701 gacgcacaag gtgtcttcca gatcctgatc cggaaccgag ccacagacct ggatgcccgc
     2761 atgcatgatg gcacgacgcc actgatcctg gctgcccgcc tggccgtgga gggcatgctg
     2821 gaggacctca tcaactcaca cgccgacgtc aacgccgtag atgacctggg caagtccgcc
     2881 ctgcactggg ccgccgccgt gaacaatgtg gatgccgcag ttgtgctcct gaagaacggg
     2941 gctaacaaag atatgcagaa caacagggag gagacacccc tgtttctggc cgcccgggag
     3001 ggcagctacg agaccgccaa ggtgctgctg gaccactttg ccaaccggga catcacggat
     3061 catatggacc gcctgccgcg cgacatcgca caggagcgca tgcatcacga catcgtgagg
     3121 ctgctggacg agtacaacct ggtgcgcagc ccgcagctgc acggagcccc gctggggggc
     3181 acgcccaccc tgtcgccccc gctctgctcg cccaacggct acctgggcag cctcaagccc
     3241 ggcgtgcagg gcaagaaggt ccgcaagccc agcagcaaag gcctggcctg tggaagcaag
     3301 gaggccaagg acctcaaggc acggaggaag aagtcccagg atggcaaggg ctgcctgctg
     3361 gacagctccg gcatgctctc gcccgtggac tccctggagt caccccatgg ctacctgtca
     3421 gacgtggcct cgccgccact gctgccctcc ccgttccagc agtctccgtc cgtgcccctc
     3481 aaccacctgc ctgggatgcc cgacacccac ctgggcatcg ggcacctgaa cgtggcggcc
     3541 aagcccgaga tggcggcgct gggtgggggc ggccggctgg cctttgagac tggcccacct
     3601 cgtctctccc acctgcctgt ggcctctggc accagcaccg tcctgggctc cagcagcgga
     3661 ggggccctga atttcactgt gggcgggtcc accagtttga atggtcaatg cgagtggctg
     3721 tcccggctgc agagcggcat ggtgccgaac caatacaacc ctctgcgggg gagtgtggca
     3781 ccaggccccc tgagcacaca ggccccctcc ctgcagcatg gcatggtagg cccgctgcac
     3841 agtagccttg ctgccagcgc cctgtcccag atgatgagct accagggcct gcccagcacc
     3901 cggctggcca cccagcctca cctggtgcag acccagcagg tgcagccaca aaacttacag
     3961 atgcagcagc agaacctgca gccagcaaac atccagcagc agcaaagcct gcagccgcca
     4021 ccaccaccac cacagccgca ccttggcgtg agctcagcag ccagcggcca cctgggccgg
     4081 agcttcctga gtggagagcc gagccaggca gacgtgcagc cactgggccc cagcagcctg
     4141 gcggtgcaca ctattctgcc ccaggagagc cccgccctgc ccacgtcgct gccatcctcg
     4201 ctggtcccac ccgtgaccgc agcccagttc ctgacgcccc cctcgcagca cagctactcc
     4261 tcgcctgtgg acaacacccc cagccaccag ctacaggtgc ctgagcaccc cttcctcacc
     4321 ccgtcccctg agtcccctga ccagtggtcc agctcgtccc cgcattccaa cgtctccgac
     4381 tggtccgagg gcgtctccag ccctcccacc agcatgcagt cccagatcgc ccgcatcccg
     4441 gaggcgttca agtaaacggc gcgtcagatc cactagtaac ggccgccagt gtgctggaat
     4501 tatcgaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     4561 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4621 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4681 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4741 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4801 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4861 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4921 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     4981 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5041 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5101 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     5161 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     5221 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     5281 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5341 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5401 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     5461 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     5521 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     5581 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     5641 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5701 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5761 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5821 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     5881 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     5941 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6001 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     6061 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     6121 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6181 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6241 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6301 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6361 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6421 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6481 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6541 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     6601 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     6661 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6721 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     6841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6901 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     6961 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     7021 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     7081 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     7141 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     7201 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     7261 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     7321 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     7381 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     7441 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     7501 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     7561 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     7621 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     7681 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     7741 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7801 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7861 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7921 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7981 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8041 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8101 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8161 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8221 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8281 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8341 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8401 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8461 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8521 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8581 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8641 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8701 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8761 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8821 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8881 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8941 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9001 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     9061 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9121 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9181 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9241 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9301 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9361 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9421 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9481 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9541 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9601 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9661 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9721 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9781 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9841 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     9901 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
     9961 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
    10021 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
    10081 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
    10141 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
    10201 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
    10261 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
    10321 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
    10381 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
    10441 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
    10501 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
    10561 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
    10621 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac t
//
