LOCUS       sequence-254613-       16534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For 
            ubiquitous genome engineering in Drosophila melanogaster..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Port F, Chen HM, Lee T, Bullock SL
  TITLE     Optimized CRISPR/Cas tools for efficient germline and somatic genome
            engineering in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
  PUBMED    25002478
REFERENCE   2  (bases 1 to 16534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2014 Jul 7. pii: 201405500."
COMMENT     Sequence Label: pAct-Cas9
FEATURES             Location/Qualifiers
     source          1..16534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(24..479)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(161..182)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     373..392
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     606..628
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     620..637
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     621..637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     644..663
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        644..662
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(696..714)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1166..1237,1639..1912,1987..2641,2703..3018,3222..3353,
                     3424..4038)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4813..4846
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4917..7427
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             9194..13297
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9194..13297
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9194..13297
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13310..13330
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          13429..13494
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13624..13644
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13916..14050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13953..13972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14007..14026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    14079..14148
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(14367..14385)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14406..14422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14406..14422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14419..14441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14430..14446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14454..14460,14461..14478,14479..14484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14499..14520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(14637..14654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14808..15396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14888..14907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15567..16358,16359..16427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16190..16209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16428..16532)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
       61 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
      121 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      181 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
      241 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
      301 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
      361 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
      421 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
      481 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      541 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      601 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
      661 gggcgaattg ggtacgtacc gggcccctag tatgtatgta agttaataaa acccattttt
      721 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
      781 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
      841 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
      901 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
      961 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     1021 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     1081 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     1141 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     1201 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     1261 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     1321 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     1381 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     1441 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     1501 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     1561 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     1621 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     1681 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     1741 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     1801 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     1861 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     1921 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     1981 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     2041 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     2101 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     2161 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     2221 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     2281 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     2341 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     2401 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     2461 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     2521 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     2581 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     2641 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     2701 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     2761 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     2821 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     2881 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     2941 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     3001 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     3061 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     3121 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     3181 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     3241 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     3301 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     3361 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     3421 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     3481 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     3541 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     3601 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     3661 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     3721 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     3781 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     3841 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     3901 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     3961 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     4021 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     4081 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     4141 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     4201 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     4261 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     4321 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     4381 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     4441 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     4501 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     4561 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     4621 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     4681 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     4741 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     4801 aactaggcta gcataacttc gtataatgta tgctatacga agttatgcta gcggatccaa
     4861 gcttgcggcc gcaattctat attctaaaaa cacaaatgat acttctaaaa aaaaatcatg
     4921 aatggcatca actctgaatc aaatctttgc agatgcacct acttctcatt tccactgtca
     4981 catcattttt ccagatctcg ctgcctgtta tgtggcccac aaaccaagac acgttttatg
     5041 gccattaaag ctggctgatc gtcgccaaac accaaataca taatgaatat gtacacattc
     5101 gagaaagaag cgatcaaaga agcgtcttcg ggcggagtag gagaatgcgg aggagaagga
     5161 gaacgagctg atctagtatc tctccacaat ccaatgccaa ctgaccaact ggccatattc
     5221 ggagcaattt gaagccaatt tccatcgcct ggcgatcgct ccattcttgg ctatatgttt
     5281 ttcaccgtta cccggggcca ttttcaaaga ctcgtcggca agataagatt gtgtcactcg
     5341 ctgtctctct tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc ggcgagtatt
     5401 ttgaagaatg agaataattt gtatttatac gaaaatcagt tagtggaatt ttctacaaaa
     5461 acatgttatc tatagataat tttgttgcaa aatatgttga ctatgacaaa gattgtatgt
     5521 atataccttt aatgtattct cattttctta tgtatttata atggcaatga tgatactgat
     5581 gatattttaa gatgatgcca gaccaaaagg cttgaatttc tgcgtctttt gccgaacgca
     5641 gtgcatgtgc aattgttgtt ttttggaata ttcaattttc ggactgtccg ctttgatttc
     5701 agtttcttgg cttattcaaa aagcaaagta aagccaaaaa agcgagatgg caataccaaa
     5761 tgcggcaaaa cggtagtgga aggaaagggg tgcggggcag cggaaggaag ggtggggcgg
     5821 ggcgtggcgg ggtctgtggc tgggcgcgac gtcaccgacg ttggagccac tcctttgacc
     5881 atgtgtgcgt gtgtgtatta ttcgtgtctc gccactcgcc ggttgttttt ttctttttat
     5941 gctgcgctct ctctagcgcc atctcgctta cgcatgctca acgcaccgca tgttgccgtt
     6001 tccttttatg cgtcattttg gctcgaaata ggcaattatt taaacaaaga ttagtcaacg
     6061 aaaacgctaa aataaataag tctacaatat ggttacttat tgccatgtgt gtgcagccaa
     6121 cgatagcaac aaaagcaaca acacaggtgg ctttccctct ttcacttttt gtttgcaagc
     6181 cgcgtgcgag caagacggca cgaccggcaa acgcaattac gctgacaaag agcagacgaa
     6241 gttttggcga aaaacatcaa ggcgcctgat acgaatgcat ttgcaataac aattgcgata
     6301 tttaatattg tttatgaagc tgtttgactt caaaacacac aaaaaaaaaa aataaaacaa
     6361 attatttgaa agagaattag gaatcggacg cttatcgtta gggtaacaac aagaaatgct
     6421 tactgagtca cagcctctgg aaaactgccg caagccagag agagagagaa aaagagggag
     6481 agcagcttag accgcatgtg cttgtgtgtg aggcgtctct ctcttcgtct ctgttgcgca
     6541 aacgcataga ctgcactgaa aaaatcgatt acctattttt tatgaatgaa tatttgcact
     6601 attactattc aaaactatta agatagcaat cacattcaat agccaaatac tataccacct
     6661 gagcgatgca acgaaatgat caatttgagc aaaaatgctg catatttagg acggcatcat
     6721 tatagaaatg cttcttgctg tgtacttttc tctcgtctgg cagctgtttc gccgttattg
     6781 ttaaaaccgg cttaagttag gtgtgttttc tacgactagt gaatgcccta ctagaagatg
     6841 tgtgttgcac aaaatgtccc tggaataacc aatttgaagt gcagatagca gtaaacgtaa
     6901 gctaatatga atattattta actgtaatgt tttaatatcg ctggacatta ctaataaacc
     6961 cactataaac acatgtacat atgtatgttt tggcatacaa tgagtagttg gggaaaaaat
     7021 gtgtaaaagc accgtgacca tcacagcata aagataacca gctgaagtat cgaatatgag
     7081 taacccccaa attgaatcac atgccgcaac tgataggacc catggaagta cactcttcat
     7141 ggcgatatac aagacacaca caagcacgaa cacccagttg cggaggaaat tctccgtaaa
     7201 tgaaaaccca atcggcgaac aattcatacc catatatggt aaaagttttg aacgcgactt
     7261 gagagcggag agcattgcgg ctgataaggt tttagcgcta agcgggcttt ataaaacggg
     7321 ctgcgggacc agttttcata tcactaccgt ttgagttctt gtgctgtgtg gatactcctc
     7381 ccgacacaaa gccgctccat cagccagcag tcgtctaatc cagagacacc aaaccgaaag
     7441 acttaattta tatttattta attaatttta ataaaacaca ccaaatgtaa gtagctttcc
     7501 ccttcccaac aacaaaacac catcgaacca ctcccaccaa gaaaaagcaa taatcgagaa
     7561 aagccgcgga aaatgtgtga ttttttttgt aaacaaaaca tttttttatg tgccagtgct
     7621 gaaagtgatc aaaaaatact agccacgagc taaagagtta ttgtattgac caaaactcca
     7681 aaaataccca agtttggccc taaattgtca atcaaaatac caataggtcg aaagacatca
     7741 aaattaacaa aaccagggtt tcaaatacca taactcaaga atcaggatta caactgcaga
     7801 tttcaggata tatacataca aattatacga aattataaaa accaaagcaa ttcaatagcc
     7861 ccaactcaaa tgttaggatc taatatagtg tttaaagcca agctcgctga tgtgggcgtg
     7921 tcacgatttc acccaaagat atgccaaatt acgaattgca aatcaattcg ccaacacttt
     7981 ctttttttcc cacgccctaa aacaccagat catccataaa tgtacataca tacagtatat
     8041 gcatattata atctgtaaaa ctagatcagg ttcttgaaaa tagtgacgta ggcagccgtt
     8101 ttggctgaag cagaaatttt tgccggtttt tcaaagttgt agttgcaaaa atggagaaaa
     8161 ccttcgagca ttcgttcata tacacacact cacgcgcaaa ataacgagag agagtgtatg
     8221 tgtgtgtgag agagcgaaag ccagacgacg gtttgctttt cgcctcgaaa catgaccata
     8281 tatggtcaca aaacttggcc gccgcaattc aacacaccag cgctctcctt cgcacccata
     8341 gcgaccatgg cgcggagcga gcgagatggc gagagcgagc gacgcctatg gcgacgtcga
     8401 cgcaggcagc gattgaaaaa cgcagttaac tggcattcaa cattcaccag ccactttcag
     8461 tcggtttatt ccagtcattc ctttcaaacc gtgcggtcgc ttagctcagc ctcgccactt
     8521 gcgtttacag tagttttcac gccttgaatt tgttaaatcg aacaaaaagg taaagtttaa
     8581 ctagctttga aaagtttcgt ggctcttaat tgttaaattt tctagagtgc gtttagtgtt
     8641 tttttttttt ttttattttg taatgttaat ttcgggttcc aattcgagtt ttaggcagcc
     8701 gccattttaa gggcgcatac acacaggcaa ctgtgctctc tttgcggctt tcttttgcac
     8761 cggcattcgt taagtgtcgt ctagaagctt ctcccctccc ttttcggcat attcgtattg
     8821 tggttttaat ttttcggggc ggggccttct attttgtaac tgttctttta atttcttatt
     8881 acaattcgat cgcaagtgaa aatcagtttt caatcggaaa agtatttttt tatgaaattt
     8941 ttttttgtcc aagattaaaa ttttgtacta aaaaaacgta cattgcattg agtgattttt
     9001 aattgtacac gaaaaacaag ttagtttgtt atgacaattg tactttggta gaccagcgca
     9061 gtccaaggaa accacgcaaa ttctcagttt tttttttgcc atttctacat taccaaataa
     9121 ggtaaccaaa aactaatggg aaatccgcat tctttccatt gcagcttaca ggatcgatcc
     9181 ccgggaattc accatggaca agaagtactc cattgggctc gatatcggca caaacagcgt
     9241 cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
     9301 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
     9361 ggagacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
     9421 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
     9481 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
     9541 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
     9601 tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct
     9661 cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
     9721 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
     9781 cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct
     9841 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
     9901 cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta aatctaactt
     9961 cgacctggcc gaagatgcca agcttcaact gagcaaagac acctacgatg atgatctcga
    10021 caatctgctg gcccagatcg gcgaccagta cgcagacctt tttttggcgg caaagaacct
    10081 gtcagacgcc attctgctga gtgatattct gcgagtgaac acggagatca ccaaagctcc
    10141 gctgagcgct agtatgatca agcgctatga tgagcaccac caagacttga ctttgctgaa
    10201 ggcccttgtc agacagcaac tgcctgagaa gtacaaggaa attttcttcg atcagtctaa
    10261 aaatggctac gccggataca ttgacggcgg agcaagccag gaggaatttt acaaatttat
    10321 taagcccatc ttggaaaaaa tggacggcac cgaggagctg ctggtaaagc ttaacagaga
    10381 agatctgttg cgcaaacagc gcactttcga caatggaagc atcccccacc agattcacct
    10441 gggcgaactg cacgctatcc tcaggcggca agaggatttc tacccctttt tgaaagataa
    10501 cagggaaaag attgagaaaa tcctcacatt tcggataccc tactatgtag gccccctcgc
    10561 ccggggaaat tccagattcg cgtggatgac tcgcaaatca gaagagacca tcactccctg
    10621 gaacttcgag gaagtcgtgg ataagggggc ctctgcccag tccttcatcg aaaggatgac
    10681 taactttgat aaaaatctgc ctaacgaaaa ggtgcttcct aaacactctc tgctgtacga
    10741 gtacttcaca gtttataacg agctcaccaa ggtcaaatac gtcacagaag ggatgagaaa
    10801 gccagcattc ctgtctggag agcagaagaa agctatcgtg gacctcctct tcaagacgaa
    10861 ccggaaagtt accgtgaaac agctcaaaga agactatttc aaaaagattg aatgtttcga
    10921 ctctgttgaa atcagcggag tggaggatcg cttcaacgca tccctgggaa cgtatcacga
    10981 tctcctgaaa atcattaaag acaaggactt cctggacaat gaggagaacg aggacattct
    11041 tgaggacatt gtcctcaccc ttacgttgtt tgaagatagg gagatgattg aagaacgctt
    11101 gaaaacttac gctcatctct tcgacgacaa agtcatgaaa cagctcaaga ggcgccgata
    11161 tacaggatgg gggcggctgt caagaaaact gatcaatggg atccgagaca agcagagtgg
    11221 aaagacaatc ctggattttc ttaagtccga tggatttgcc aaccggaact tcatgcagtt
    11281 gatccatgat gactctctca cctttaagga ggacatccag aaagcacaag tttctggcca
    11341 gggggacagt cttcacgagc acatcgctaa tcttgcaggt agcccagcta tcaaaaaggg
    11401 aatactgcag accgttaagg tcgtggatga actcgtcaaa gtaatgggaa ggcataagcc
    11461 cgagaatatc gttatcgaga tggcccgaga gaaccaaact acccagaagg gacagaagaa
    11521 cagtagggaa aggatgaaga ggattgaaga gggtataaaa gaactggggt cccaaatcct
    11581 taaggaacac ccagttgaaa acacccagct tcagaatgag aagctctacc tgtactacct
    11641 gcagaacggc agggacatgt acgtggatca ggaactggac atcaatcggc tctccgacta
    11701 cgacgtggat catatcgtgc cccagtcttt tctcaaagat gattctattg ataataaagt
    11761 gttgacaaga tccgataaaa atagagggaa gagtgataac gtcccctcag aagaagttgt
    11821 caagaaaatg aaaaattatt ggcggcagct gctgaacgcc aaactgatca cacaacggaa
    11881 gttcgataat ctgactaagg ctgaacgagg tggcctgtct gagttggata aagccggctt
    11941 catcaaaagg cagcttgttg agacacgcca gatcaccaag cacgtggccc aaattctcga
    12001 ttcacgcatg aacaccaagt acgatgaaaa tgacaaactg attcgagagg tgaaagttat
    12061 tactctgaag tctaagctgg tctcagattt cagaaaggac tttcagtttt ataaggtgag
    12121 agagatcaac aattaccacc atgcgcatga tgcctacctg aatgcagtgg taggcactgc
    12181 acttatcaaa aaatatccca agcttgaatc tgaatttgtt tacggagact ataaagtgta
    12241 cgatgttagg aaaatgatcg caaagtctga gcaggaaata ggcaaggcca ccgctaagta
    12301 cttcttttac agcaatatta tgaatttttt caagaccgag attacactgg ccaatggaga
    12361 gattcggaag cgaccactta tcgaaacaaa cggagaaaca ggagaaatcg tgtgggacaa
    12421 gggtagggat ttcgcgacag tccggaaggt cctgtccatg ccgcaggtga acatcgttaa
    12481 aaagaccgaa gtacagaccg gaggcttctc caaggaaagt atcctcccga aaaggaacag
    12541 cgacaagctg atcgcacgca aaaaagattg ggaccccaag aaatacggcg gattcgattc
    12601 tcctacagtc gcttacagtg tactggttgt ggccaaagtg gagaaaggga agtctaaaaa
    12661 actcaaaagc gtcaaggaac tgctgggcat cacaatcatg gagcgatcaa gcttcgaaaa
    12721 aaaccccatc gactttctcg aggcgaaagg atataaagag gtcaaaaaag acctcatcat
    12781 taagcttccc aagtactctc tctttgagct tgaaaacggc cggaaacgaa tgctcgctag
    12841 tgcgggcgag ctgcagaaag gtaacgagct ggcactgccc tctaaatacg ttaatttctt
    12901 gtatctggcc agccactatg aaaagctcaa agggtctccc gaagataatg agcagaagca
    12961 gctgttcgtg gaacaacaca aacactacct tgatgagatc atcgagcaaa taagcgaatt
    13021 ctccaaaaga gtgatcctcg ccgacgctaa cctcgataag gtgctttctg cttacaataa
    13081 gcacagggat aagcccatca gggagcaggc agaaaacatt atccacttgt ttactctgac
    13141 caacttgggc gcgcctgcag ccttcaagta cttcgacacc accatagaca gaaagcggta
    13201 cacctctaca aaggaggtcc tggacgccac actgattcat cagtcaatta cggggctcta
    13261 tgaaacaaga atcgacctct ctcagctcgg tggagacagc agggctgacc ccaagaagaa
    13321 gaggaaggtg tgactcgagg gtacctctag aggatctttg tgaaggaacc ttacttctgt
    13381 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
    13441 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
    13501 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
    13561 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
    13621 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
    13681 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
    13741 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc
    13801 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
    13861 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
    13921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
    13981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    14041 tgtctggatc cactagtgtc gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc
    14101 cagggcgtgc ccttgggctc cccgggcgcg tactccacct cacccatctg gtccatcatg
    14161 atgaacgggt cgaggtggcg gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc
    14221 tcgccctcga aaccgctggg cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg
    14281 gcgggtgcgg atacgcgggg cagcgtcagc gggttctcga cggtcacggc gggcatgtcg
    14341 acactagttc tagccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
    14401 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    14461 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    14521 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    14581 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    14641 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    14701 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    14761 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    14821 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    14881 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    14941 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    15001 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    15061 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    15121 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    15181 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    15241 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    15301 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    15361 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    15421 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    15481 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    15541 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    15601 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    15661 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    15721 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    15781 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    15841 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    15901 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    15961 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    16021 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    16081 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    16141 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    16201 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    16261 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    16321 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    16381 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    16441 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    16501 atttagaaaa ataaacaaat aggggttccg cgca
//
