LOCUS       sequence-254623-       10220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dox-dependent expression of mouse Gata6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10220)
  AUTHORS   Benchetrit H, Jaber M, Zayat V, Sebban S, Pushett A, Makedonski K, 
            Zakheim Z, Radwan A, Maoz N, Lasry R, Renous N, Inbar M, Ram O, 
            Kaplan T, Buganim Y
  TITLE     Direct Induction of the Three Pre-implantation Blastocyst Cell Types
            from Fibroblasts.
  JOURNAL   Cell Stem Cell. 2019 Jun 6;24(6):983-994.e7. doi: 
            10.1016/j.stem.2019.03.018. Epub 2019 Apr 25.
  PUBMED    31031139
REFERENCE   2  (bases 1 to 10220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.03.018"; journalName: "Cell Stem Cell"; date: 
            "2019-06-6- 6"; volume: "24"; issue: "6"; pages: "983-994.e7"
COMMENT     Sequence Label: FUW-TetO-Gata6
FEATURES             Location/Qualifiers
     source          1..10220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..49
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    347..464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(521..539,540..562,563..581,582..604,605..623,624..646,
                     647..665,666..688,689..707,708..730,731..749,750..772,
                     773..791)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        824..862
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     859..883
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1970..1990
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    2819..3407
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2872..2892)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3290..3301)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3410..3426)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3411..3427)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3932..4112
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4138..4155)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4144..4368
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4414..4842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4501..4520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4711..4732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4851..4871)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4856..5185
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5036..5171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5098..5117
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5233..5280
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5299..5673
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5803..5924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5840..5859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5894..5913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5973..5989)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5973..5989)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5986..6008)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5997..6013
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6021..6027,6028..6045,6046..6051))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6066..6087
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6204..6221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6375..6963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6455..6474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7134..7925,7926..7994))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7757..7776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7995..8099)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8174..8193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8365..8744
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8746..8944
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             8962..9142
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9189..9314
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9807..10040
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
       61 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
      121 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
      181 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
      241 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc
      301 actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      361 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      421 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      481 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
      541 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      601 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      661 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      721 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
      781 agtgatagag aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg
      841 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      901 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt
      961 gcgctcgagg aatcaaaagt caggttggag tagcgccgga cagtggatgg ccttgactga
     1021 cggcggctgg tgcctgccaa agcgtttcgg ggctgctgct gcggacgccg gcgactccgg
     1081 gccctttcca gcgcgggagc cctcctcgcc gctttccccc atctcgtctt cgtcctcctc
     1141 ctgctcccgg ggcggggatc gcggtccctg cggcgccagc aactgcagga cgccgcagct
     1201 cgacgccgag gcggtggcgg gacctccggg ccgctcgctc ttgctcagcc cctacgcctc
     1261 gcatcccttc gccgctgccc acggagccgc ggcgcccggg gtcgcaggcc ccgggagcgc
     1321 cctgtcgact tgggaggacc tgttgctctt cactgacctc gatcaggccg cgaccgccag
     1381 caagctgttg tggtccagcc ggggcgccaa actgagcccc ttcgcggccg agcagccgga
     1441 ggaaatgtac cagaccctcg ccgccctgtc cagccagggg cccgccgctt acgacggcgc
     1501 gcccggcggc ttcgtgcact ccgcagcggc ggcggccgct gccgccgcgg cagccagctc
     1561 cccggtctac gtgcccacca cgcgcgtggg ctccatgctg tccggcctgc cctaccttca
     1621 aggggcgggc agcgggccca gcaatcacgc gggcggagcg ggtgcccacc caggctggtc
     1681 ccaggcctcc gccgacagcc ccccgtatgg cgggggtggc gcagccggcg gcggcgcggc
     1741 cggacctgga ggtgcgggat cggctacggc ccacgcctct gcacgctttc cctactcgcc
     1801 cagcccgccc atggccaacg gcgccgcgcg aaaccccggg ggctacgtgg ctgcgggcgg
     1861 cacgggcgca ggcagtgtga gtggaggtgg cggcagcctg gcggccatgg gtggccggga
     1921 gcaccagtac agctcgctgt ccgcagctcg gccgctgaac ggaacgtacc accaccacca
     1981 tcaccatcac ccgacctact cgccctacat ggccgcaccg ctgactcctg cctggccagc
     2041 aggacccttc gaaacgccgg tgctccacag cttacagggc cgcgcgggag ctccactccc
     2101 ggtgccacgg ggccccagca cagacctgtt ggaggacctg tcggagagcc gcgagtgcgt
     2161 gaactgcggc tccatccaga cgccactgtg gagacgagac ggcaccggtc attacctgtg
     2221 caatgcatgc ggtctctaca gcaagatgaa tggcctcagc aggcccctca tcaagccaca
     2281 gaagcgcgtg ccttcatcac ggcggcttgg actgtcctgt gccaactgtc acaccacaac
     2341 cactacctta tggcgtagaa atgctgaggg tgagcctgtg tgcaatgctt gcgggctcta
     2401 tatgaaactc catggggtgc ctcgaccact tgctatgaaa aaagaaggaa ttcaaaccag
     2461 gaaacgaaaa cctaaaaata taaataagtc aaaagcttgc tccggtaaca gcagtggctc
     2521 tgtccctatg actcctactt cctcttcttc taattcagat gactgcacca aaaatacttc
     2581 tccttctaca caagcgacca cctcaggggt aggggcatca gtgatgtctg cagtgggaga
     2641 aaacgccaac cccgagaaca gtgacctcaa gtattcaggt caagacggcc tctacatagg
     2701 tgtcagtctg tcctcccctg ccgaagtcac atcctccgtg cgacaggatt cttggtgtgc
     2761 tctggccctg gcctgagctg gtgctaccaa gaggcaattc gatatcaagc ttatcgataa
     2821 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     2881 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     2941 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3001 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3061 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3121 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3181 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3241 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3301 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3361 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     3421 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     3481 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     3541 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     3601 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     3661 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     3721 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt
     3781 agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat
     3841 ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca
     3901 catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc
     3961 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     4021 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     4081 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     4141 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     4201 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     4261 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     4321 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     4381 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     4441 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4501 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4561 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4621 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4681 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4741 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4801 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     4861 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     4921 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4981 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     5041 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     5101 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     5161 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     5221 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
     5281 aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc
     5341 gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg
     5401 gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag
     5461 gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg
     5521 tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg
     5581 accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac
     5641 tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc
     5701 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     5761 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     5821 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5881 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     5941 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6001 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6061 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6121 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6181 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6241 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6361 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6541 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6781 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7261 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8101 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     8161 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8221 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8281 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8341 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
     8401 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     8461 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     8521 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     8581 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     8641 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     8701 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     8761 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     8821 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     8881 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     8941 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     9001 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     9061 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     9121 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     9181 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     9241 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     9301 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     9361 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     9421 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     9481 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     9541 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     9601 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     9661 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     9721 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     9781 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     9841 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     9901 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     9961 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
    10021 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
    10081 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
    10141 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
    10201 gaagaatcgc aaaaccagca
//
