LOCUS       sequence-25463-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC245
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..49)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(71..91)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(113..133)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(155..175)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    251..298
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    323..446
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(471..476,477..494,495..501)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 cttctccttt actcattttt tctaccggta ccttacgctt cttctttgga gcagtcattt
       61 tttctgccgg taccttacgc ttcttctttg gagcagtcat tttttctgcc ggtaccttac
      121 gcttcttctt tggagcagtc attttttctg ccggtacctt acgcttcttc tttggagcag
      181 tcattttttc tgagctcctg aaaatgttct atgttatgtt agtatcattc gaaacatacc
      241 cggggatctt gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcag
      301 agcggctagc ccagtgaatt atcaactatg tataataaag ttgaacgaga aacgtaaaat
      361 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt
      421 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt
      481 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag
      541 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat a
//
