LOCUS       sequence-254632-       12808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 433, Emission: 475.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Markwardt ML, Kremers GJ, Kraft CA, Ray K, Cranfill PJ, Wilson KA, 
            Day RN, Wachter RM, Davidson MW, Rizzo MA
  TITLE     An improved cerulean fluorescent protein with enhanced brightness 
            and reduced reversible photoswitching.
  JOURNAL   PLoS One. 2011  . 6(3):e17896.
  PUBMED    21479270
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2011  . 6(3):e17896."
COMMENT     Sequence Label: mCerulean3-Talin-18
FEATURES             Location/Qualifiers
     source          1..12808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             55..849
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             55..849
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(109..128)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     719..738
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1037..1056)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1081..1128
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1457..2045
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1946..1965
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2220..2523
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2524..2727
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2678..2698
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2766..2775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2772..2774,2775..2777,2778..3488)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2817..2838)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3078..3097)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3425..3446
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      3537..3546
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    11755..11876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11792..11811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11846..11865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(11883..12338)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12020..12041)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12232..12251
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        12365..12469
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(12471..12808,1..20)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        12471..12662
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(12494..12514)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(12679..12808,1..6)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
       61 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
      121 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      181 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
      241 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      301 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      361 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      421 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      481 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      541 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
      601 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
      661 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
      721 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
      781 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
      841 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
      901 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
      961 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1021 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     1081 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
     1141 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
     1201 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
     1261 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
     1321 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1381 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1441 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1501 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1561 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1621 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1681 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1741 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1801 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1861 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1921 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1981 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2041 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2101 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcatt
     2161 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2221 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2281 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2341 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2401 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2461 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2521 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2581 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2641 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2701 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
     2761 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     2821 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2881 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2941 cctggcccac cctcgtgacc accctgagct ggggcgtgca gtgcttcgcc cgctaccccg
     3001 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3061 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3121 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3181 tcctggggca caagctggag tacaacgcca tccacggcaa cgtctatatc accgccgaca
     3241 agcagaagaa cggcatcaag gccaacttcg gcctcaactg caacatcgag gacggcagcg
     3301 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3361 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     3421 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3481 tgtacaagtc cggactcaga tctcgagctc aagctagctt cgaattcgca gaagctgcca
     3541 ccatggttgc gctttcgctg aagattagca ttgggaatgt ggtgaagacg atgcaatttg
     3601 agccatctac catggtgtac gacgcctgcc gcatgattcg tgagcggatc ccagaggccc
     3661 tggctggccc tcccaacgac tttgggctct ttctgtcaga tgatgacccc aaaaaaggca
     3721 tctggctaga ggctgggaaa gctttggact actacatgct ccgaaatggg gacaccatgg
     3781 agtacagaaa aaagcagaga cccctgaaga tacggatgtt agacggaacg gtgaagacta
     3841 tcatggtgga tgactccaag accgtcacag acatgctcat gaccatttgt gcccgaattg
     3901 gtatcaccaa ccatgatgag tattcactgg ttcgagagct gatggaagaa aagaaagatg
     3961 aggggacagg gaccctgaga aaagacaaga ccctactgcg agatgaaaag aagatggaga
     4021 agctaaagca gaagttgcac acagacgatg agttgaactg gctggaccat gggcggacac
     4081 tgagggaaca gggagtggaa gagcatgaga cgttgctgct gcggagaaag ttcttctact
     4141 cagaccagaa tgtggattcc cgagaccctg tacagctgaa ccttctctat gtgcaggcac
     4201 gagatgacat cctgaatggc tcccatcctg tctcctttga caaggcctgt gaatttgcag
     4261 gcttccagtg ccagatccag tttggacctc acaatgaaca gaagcacaag gctggcttcc
     4321 ttgacctgaa ggacttcctg cccaaggagt atgtgaagca gaagggagag cgtaagatct
     4381 ttcaggcaca caagaattgt gggcagatga gtgaaattga ggccaaggta cgctacgtga
     4441 agctggcccg ttccctcaag acttacggtg tctccttctt cctagtcaag gaaaagatga
     4501 aggggaagaa taaactggtg cccaggctgt tgggcatcac taaggagtgt gtgatgcgtg
     4561 tggatgagaa gaccaaggag gtgattcagg aatggagcct caccaacatc aaacgctggg
     4621 ctgcctctcc caagagcttt actctggact ttggagacta tcaggatggc tactactcag
     4681 tacagacgac agaaggcgag cagatcgcac agctcattgc tggctacata gatatcatcc
     4741 ttaagaagaa aaaaagcaag gaccattttg ggctggaggg agatgaagag tctactatgt
     4801 tggaggactc agtgtctccc aaaaagtcaa cagtccttca gcagcagtac aaccgagtgg
     4861 ggaaagtgga gcacggctct gtggctctgc cagccatcat gcgctctgga gcctctggtc
     4921 ctgagaattt ccaggtgggg agcatgccac ctgcccagca gcagatcacc agtggccaga
     4981 tgcaccgagg acacatgcca cctctgactt cagcccagca ggcgctcact ggaaccatta
     5041 actccagcat gcaggctgtg caggctgccc aggccactct ggatgacttt gagactctac
     5101 cccctcttgg ccaggatgct gcctccaaag cttggcgtaa gaacaagatg gatgaatcaa
     5161 agcatgagat ccactcccag gtagatgcca tcacagctgg cactgcctct gtggtaaacc
     5221 tgacagcagg agatcctgca gagacagact atacagcagt gggctgtgca gtcaccacca
     5281 tctcttccaa cctgacggag atgtcccgtg gggtgaagct attggctgcc ctgctggagg
     5341 atgaaggcgg caatggccgg ccccttctgc aagcagcaaa gggccttgca ggggctgtgt
     5401 cggaactgct tcgcagtgct cagcccgcca gcgctgagcc tcgtcagaac ctgctgcagg
     5461 cagccgggaa cgtgggccag gccagtgggg agctgttgca gcaaattggg gaaagtgaca
     5521 ctgaccccca cttccaggat gttctaatgc agctagcaaa cgcagtggca agtgctgcag
     5581 ccgccctggt cctcaaggcc aagagtgtgg cccagcgcac agaggattcc gggcttcaga
     5641 cccaagtgat tgctgcagct acgcaatgtg ctctgtccac ttcccagctg gtggcctgta
     5701 ccaaggtggt agcacctaca atcagctcac ctgtgtgcca agaacagcta gtcgaggctg
     5761 ggcgactggt ggccaaagct gtggagggct gtgtgtctgc ctcgcaagca gctacagaag
     5821 atggacagct gcttcgaggg gtaggagcag cagccacggc tgtcacccag gccctcaatg
     5881 agctgctgca gcacgtgaag gcccacgcca ccggagctgg gcctgccggt cggtatgacc
     5941 aagccactga taccatcctc accgtcactg aaaacatctt cagctccatg ggtgatgctg
     6001 gggagatggt gcgccaggcc cgcatcctgg cccaagccac ctcagacctg gtcaatgcta
     6061 tcaaggccga tgctgagggg gagagtgatc tggagaactc tagaaagctc ctgagtgctg
     6121 ccaagatcct cgctgatgcc accgccaaga tggtggaggc ggccaaggga gcagccgccc
     6181 accctgacag tgaggaacag cagcagcgac tgcgtgaagc agctgagggg cttcgcatgg
     6241 ccaccaatgc agctgcgcag aacgccatca agaagaagtt ggtgcagcgc ctggagcatg
     6301 cagccaagca agctgcagcc tctgcaacac agaccattgc tgcagcccaa catgcagcct
     6361 ctgcccccaa ggcctctgcg ggcccccagc ccctactggt gcagagctgt aaggccgtgg
     6421 cagagcagat tccgttgctg gtgcagggtg tccgaggaag tcaagctcag cctgacagcc
     6481 ctagtgctca gctcgccctc attgctgcca gccagagctt cctgcagcca ggtgggaaga
     6541 tggtggcagc ggcaaaagcc tcagtgccaa caattcagga ccaggcttca gcgatgcagc
     6601 tgagtcagtg cgcaaagaac ctaggcactg ccctggccga gctgcgcact gctgctcaga
     6661 aggctcagga ggcatgtgga cctttggaga tggattctgc actgagtgtt gtacaaaatc
     6721 tagagaaaga cctacaggaa ataaaagcag cagctcgaga tggcaagctt aagcccttac
     6781 ctggggaaac aatggagaag tgtacccaag atcttggcaa cagcaccaaa gcggtgagct
     6841 ctgccatcgc caagctgctg ggggagattg cccagggcaa cgagaactat gcaggtattg
     6901 cagctcggga tgtagctggt ggactaaggt cactagccca ggctgcgcgg ggtgtggctg
     6961 cgctgacatc agatcctgca gtgcaggcca ttgtgcttga cacagccagc gacgttctgg
     7021 acaaggccag cagcctcatt gaagaggcaa aaaaagcatc tggacaccca ggggacccag
     7081 aaagccagca aaggcttgct caggtagcta aagcagtgac ccaagccctg aaccgctgtg
     7141 tcagctgcct gcctggccaa agagacgtgg ataatgccct acgagccgtt ggagatgcca
     7201 gcaagcgact cctgagtgac ttgcttcctc caagcacggg gacatttcaa gaagcacaga
     7261 gccgattgaa tgaagctgca gctgggttaa atcaggccgc cacagaactg gtgcaagcct
     7321 cccgaggaac tcctcaggac ctggctcggg cctccggccg atttggacag gacttcagca
     7381 ccttcctgga agctggtgtg gagatggccg gacaggcccc gagccaggag gaccgagccc
     7441 aggtggtgtc caacctgaag ggcatatcca tgtcttcaag caaacttctc cttgccgcta
     7501 aggccttgtc cacagaccct gcttctccca acctcaagag tcagctggct gcggctgccc
     7561 gggcagtgac ggacagcatc aaccagctca tcaccatgtg cacccagcag gcacctggcc
     7621 agaaggagtg tgacaatgca cttcggcagc tagagacagt ccgagaactc ctggagaatc
     7681 cagtccagcc catcaacgac atgtcctact tcggttgctt ggacagtgtc atggagaact
     7741 ccaaggtcct aggtgaggcc atgactggca tctcccaaaa tgccaagaat ggaaatctgc
     7801 cggagtttgg agatgccatt gccacagcct ccaaggctct ctgtggcttc acggaggcag
     7861 ccgcacaggc agcatatcta gttggtgtct ctgaccccaa cagccaagct ggacagcaag
     7921 gactggtgga acccacacag tttgcccgtg caaaccaggc aattcagatg gcctgtcaga
     7981 gtctggggga gcctggctgt acccaggccc aggtgttatc tgcagccact attgtagcca
     8041 aacacacatc tgcattgtgt aacagctgtc gcctggcttc cgctaggact gccaatccca
     8101 ctgccaagcg ccagtttgta cagtcagcca aggaggtggc caacagtaca gccaatcttg
     8161 tcaagaccat caaggcacta gatggggact tcacagaaga gaaccgtgcc cagtgccgag
     8221 cagccacagc ccctctgctg gaagctgtgg ataacctgag tgcctttgcc tccaaccctg
     8281 agttctccag cgtccctgcc cagatcagcc ctgagggcag agcagccatg gagcccattg
     8341 taatctctgc taagacaatg ttggagagtg ctggagggct catccagaca gctcgggcgt
     8401 tagcagtcaa tccccgagac cccccacgtt ggtctgtgct agctggccac tctcggactg
     8461 tctcagattc catcaagaaa cttattacaa gcatgaggga caaagcccca gggcagctgg
     8521 agtgtgagac agccattgcg gctctgaaca gctgcttgcg ggacctagat caggcttcgc
     8581 ttgccgctgt cagccagcag cttgctcccc gtgaaggaat ctctcaagag gctttgcaca
     8641 cccagatgct cactgcagtg caagaaattt ctcatctcat tgagccgctg gccagcgctg
     8701 ctcgggctga agcctcccag ctgggacaca aggtgtccca aatggcccag tactttgaac
     8761 cactcaccct ggctgcagtg ggtgctgcgt ctaagaccct gagccacccg cagcagatgg
     8821 cacttctgga ccagactaaa acgttggcag agtctgcctt gcagttgcta tatactgcca
     8881 aggaggctgg tggtaacccc aagcaagcag cacacaccca ggaagccctg gaggaggctg
     8941 tgcagatgat gacagaggcc gtagaagacc tgacgacgac cctcaacgaa gcagccagtg
     9001 ctgcaggagt cgttggcggc atggtggact ctatcactca ggccatcaac cagctagatg
     9061 aaggacctat gggtgaccca gaaggctcat tcgtagatta ccagacaacc atggtgagga
     9121 cagccaaggc cattgctgtc actgttcagg agatggtaac caagtcaaac accagccctg
     9181 aagagctagg ccctcttgcc aaccagctga ccagtgacta tggccgactg gcctcacaag
     9241 ccaagcctgc agctgtggct gctgaaaatg aagagatagg cgctcacatc aaacaccgag
     9301 tacaggagct gggccatggc tgctctgctc tggtcaccaa ggcaggtgcc ttgcagtgta
     9361 gccccagtga tgtctacacc aagaaggagc tcatagagtg tgcccgcaga gtgtcagaga
     9421 aggtctccca tgttctggct gcactccagg ctgggaatcg tggtacccag gcctgcatta
     9481 cagcagccag tgctgtgtct ggtatcattg ctgacctcga caccaccatc atgttcgcta
     9541 ctgctggcac acttaaccgt gagggtgctg aaacttttgc tgaccaccgg gagggtatct
     9601 taaagacagc aaaggttctt gtggaggaca ccaaggtcct agtgcagaat gcagctggga
     9661 gccaggagaa gttggcacaa gccgcccagt cctccgtggc caccattacc cgcctcgctg
     9721 atgtggtcaa gctcggtgca gccagcctag gagccgaaga ccctgaaact caggtggtgc
     9781 tgatcaatgc agtaaaggac gtagccaagg ccctgggtga cctcatcagc gctacgaagg
     9841 ctgcagcggg caaagttggg gatgaccctg cagtgtggca gctcaagaac tctgccaagg
     9901 tgatggtgac caatgtgaca tcattgctca agacagtgaa ggctgtggaa gatgaggcca
     9961 ccaaaggcac acgggcccta gaggcaacca cagagcacat acgtcaggaa ctggcggtct
    10021 tctgttcccc agagccacct gccaagacct ctacccctga agacttcatc cgaatgacca
    10081 agggtattac tatggcaaca gccaaagccg ttgctgctgg caattcctgt cgacaggaag
    10141 atgtcattgc cacagccaat ctgagccgac gggctattgc ggacatgctt cgggcttgca
    10201 aggaagcagc tttccaccca gaagtggcgc ctgatgtacg gctccgagcc ctgcactatg
    10261 gccgggagtg tgccaatggt tacctggaac tgctggacca cgtgctgctg acccttcaga
    10321 agccaaaccc agacctgaag cagcagctaa ctggacactc aaagcgggtt gctggctctg
    10381 tgactgagct catccaggct gctgaagcca tgaaaggaac agagtgggtg gacccagagg
    10441 accctactgt cattgctgag aatgagctct tgggagccgc agccgccatc gaggctgcag
    10501 ccaagaagct ggagcagctg aagcccaggg ccaaacccaa ggaggcggat gagtccttga
    10561 actttgagga acaaatccta gaagctgcca agtccatcgc tgcagccacc agtgcactgg
    10621 taaaggctgc gtcagcagcc cagagggagc tggtggctca aggaaaggtg ggcgccattc
    10681 cagccaatgc actggatgat gggcagtggt cgcagggcct catttccgct gcccgtatgg
    10741 tggctgcggc caccaacaat ctgtgtgaag cagctaacgc agctgtccag ggccatgcta
    10801 gccaggagaa actcatttcc tcagccaagc aagtagctgc ctccacagct cagctcctgg
    10861 tagcttgcaa ggtcaaagca gatcaggact ctgaggcaat gaaacggctc caggctgctg
    10921 gcaatgcagt gaagagggcc tcagataacc tggtaaaggc ggcccagaaa gctgcagcct
    10981 tcgaagacca ggagaatgag acggtggtgg tgaaggagaa aatggttggg ggcattgccc
    11041 agattatcgc agcacaggaa gagatgcttc ggaaggaacg agagctggaa gaggctcgga
    11101 aaaagctcgc ccagatccgg cagcagcagt acaagttctt gccttcagag cttcgagacg
    11161 agcactaaaa aagccctgtg tatttaatgc agacccagcc cagagactgt gcctgccact
    11221 accaaagcct tctgggctgc tcaggaccca agctgcccaa ccccagcact cccccaaagt
    11281 gcctgccaaa ccctgggcct ggccctgccc agtcccactg caagccgtgt cctctccctg
    11341 actcccaagt gcctttgcac cctagggccc ctaagtgcct gcccccctcc agagtattaa
    11401 cgctccaaga gtattattaa tgctgccgta cctcagtttg aacctgccag ggccccagct
    11461 gctccagcct gccagcagct tccagccagt ccccacggcc acgtcagctc aactcatccc
    11521 tttttgatac tatatcccct acccagctac ctatggggct tgagggttgt aaacccaaac
    11581 aggtcagact cccggaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
    11641 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    11701 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    11761 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    11821 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
    11881 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
    11941 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
    12001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
    12061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
    12121 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
    12181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
    12241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
    12301 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
    12361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    12421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
    12481 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    12541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    12601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    12661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    12721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    12781 ttccagaagt agtgaggagg cttttttg
//
