LOCUS       sequence-254633-       12503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  brr2-C520R (endogenous promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12503)
  AUTHORS   Mayerle M, Guthrie C
  TITLE     Brr2 is a splicing fidelity factor
  JOURNAL   bioRxiv 2018. 354514
REFERENCE   2  (bases 1 to 12503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2018. 354514"
COMMENT     Sequence Label: brr2-C520R pRS313
FEATURES             Location/Qualifiers
     source          1..12503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(94..281)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        703..807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(808..876,877..1668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1026..1045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1839..2427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2328..2347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2581..2598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2715..2736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2751..2756,2757..2774,2775..2781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2789..2805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2794..2816
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2813..2829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2813..2829
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9967..9984)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9967..9983)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9976..9998)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    10498..10790
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      10791..11244
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     CDS             complement(join(11934..12503,1..93))
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 ctctatcgct aggggaccac cctttaaaga gatcgcaatc tgaatcttgg tttcatttgt
       61 aatacgcttt actagggctt tctgctctgt catctttgcc ttcgtttatc ttgcctgctc
      121 attttttagt atattcttcg aagaaatcac attactttat ataatgtata attcattatg
      181 tgataatgcc aatcgctaag aaaaaaaaag agtcatccgc taggtggaaa aaaaaaaatg
      241 aaaatcatta ccgaggcata aaaaaatata gagtgtacta gaggaggcca agagtaatag
      301 aaaaagaaaa ttgcgggaaa ggactgtgtt atgacttccc tgactaatgc cgtgttcaaa
      361 cgatacctgg cagtgactcc tagcgctcac caagctctta aaacgagaat taagaaaaag
      421 tcgtcatctt tatactaagt tggcattata aaaaagcatt gcttatcaat ttgttgcaac
      481 gaacaggtca ctatcagtca aaataaaatc attatttgat ttcaattttg tcccactccc
      541 tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttatgcccg agaagatgtt
      601 gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg cgcaactcgt
      661 gaaaggtagg cggatccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
      721 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
      781 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      841 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      901 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      961 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     1021 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     1081 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     1141 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     1201 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     1261 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1321 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1381 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1441 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1501 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     1561 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     1621 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1681 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1741 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1801 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1861 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1921 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1981 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     2041 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     2101 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     2161 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2221 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2281 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2341 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2401 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2461 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2521 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2581 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     2641 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgagcagt
     2701 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     2761 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     2821 agctatgaca tgattacgaa ttcgagctcc attttgttat ttgcgagccc aatgaactga
     2881 gactatattg taataagggc cgtttgttca aattcgtact atccatatca ttcagacagg
     2941 aacaaacttg ataattagtg gtgttgaatc aagtactatt aacgcattgt gatgatctct
     3001 cagatatcaa tgcgtgtttg cttctttgac gatctaatat tgaatattgt cgctctttac
     3061 gagaatatta aaaatttatg ctactaaagc tatgtctact ataaaggaat aatacactaa
     3121 tagagataat cttgagtaaa ataaggtgaa ggtaaatata agtaattgct ttggaagatt
     3181 taccgtgagc cttcgttatt aaaaatgact gagcatgaaa cgaaggataa ggccaaaaaa
     3241 attagagaaa tttatcgtta tgatgagatg tccaataagg tgttgaaagt tgataaacgc
     3301 tttatgaata ccagccaaaa tcctcaaagg gatgcggaaa tatcacaacc aaaatccatg
     3361 agtggtagaa ttagtgctaa ggatatggga cagggcctgt gcaataacat taataaagga
     3421 ctaaaagaaa atgatgtagc cgttgaaaaa actggaaaga gcgcatcatt gaaaaaaatt
     3481 cagcagcata acaccatttt gaattcttcc tccgatttca ggttacatta ttatccaaag
     3541 gatccctcaa atgtcgaaac ttacgagcag attttgcagt gggttacaga agttttaggc
     3601 aatgatatac cccatgatct tattattgga actgctgata tattcatcag acaattgaaa
     3661 gaaaatgagg aaaatgagga tggtaatatt gaagagcgaa aggaaaagat ccagcatgag
     3721 ctaggcatca atatagactc cctgaagttt aacgaattag ttaaacttat gaaaaacatt
     3781 accgattatg aaacacatcc tgacaatagc aataaacaag cagtagcaat tcttgccgat
     3841 gatgaaaaat cagatgaaga agaagtaaca gaaatgtcca acaatgctaa tgttcttggg
     3901 ggagaaatca atgataatga agatgatgat gaggaatatg attacaatga cgttgaagtc
     3961 aattctaaaa aaaaaaacaa gagggcattg ccaaacattg aaaacgatat aattaaactc
     4021 tccgatagta aaacaagtaa tatcgagagc gttccgatat actcaatcga tgaatttttt
     4081 ttgcagagaa aacttcggtc agagcttggt tacaaggata cctctgtaat acaagacctg
     4141 tcagaaaaaa ttttgaatga tattgaaaca cttgagcata atccagtagc gttggagcag
     4201 aagttggttg atctgcttaa attcgagaat atatcactgg ctgaatttat tctcaagaat
     4261 agatctacaa ttttttgggg catacgactg gcgaagagca cagagaatga aataccgaac
     4321 ttaatagaga aaatggttgc aaaaggtttg aatgatcttg tggaacagta taaatttaga
     4381 gaaactacac actcaaagcg tgaactcgat tccggtgacg accagccaca gtcttcggaa
     4441 gcaaaaagaa caaaattcag taatccagca ataccgcccg tcatagattt ggaaaagatt
     4501 aaattcgacg aaagttctaa attaatgacc gtgacaaaag tatccctacc ggaaggatca
     4561 ttcaaaaggg tgaaaccaca atatgacgaa atacatattc cagctcctag caaacctgtt
     4621 attgattatg agttgaaaga aattacttct ctaccagatt ggtgtcagga ggcgtttcct
     4681 tcatcggaaa caacgtcctt aaaccctata caatcaaagg tttttcacgc agcatttgaa
     4741 ggtgattcaa acatgctaat acgtgcaccc actggttcag gtaagacgaa tatcgcgtta
     4801 ttgacggtat tgaaagcctt atcccatcat tataacccga aaactaagaa attaaacctt
     4861 tctgcattta aaattgttta tatagcaccc ttgaaagcct tagtacagga acaagtccga
     4921 gaatttcaaa ggaggttggc ttttctaggt ataaaagtcg ctgaattgac tggtgattct
     4981 aggcttagta gaaagcaaat tgacgagacg caggttttgg tgtctacgcc agagaaatgg
     5041 gacataacta ctagaaattc aaataatttg gccatcgtag aacttgttcg tttgttgatt
     5101 atagatgaaa tacatttgtt acatgatgac aggggaccag tattggaaag catcgtggct
     5161 aggacatttt gggcttccaa atatggccag gaatacccca gaataatagg cctttctgcc
     5221 accttaccaa attatgagga tgtcggtagg tttttaagag ttccgaagga aggattgttt
     5281 tattttgact cctcatttag accgtgtcct ctatctcaac agttttgtgg cattaaggaa
     5341 cggaactcct tgaaaaagct gaaggctatg aatgatgctt gttatgaaaa ggtattggaa
     5401 tccatcaatg aaggtaatca aataatcgtg tttgttcatt ctcgaaaaga aacatcacga
     5461 acagcaacat ggttgaaaaa taaatttgct gaagagaaca ttactcataa attaaccaaa
     5521 aatgatgcag gatcgaagca gattttaaag acagaagcag ccaacgtact cgatccaagt
     5581 ctacgaaagt tgattgaaag cggtataggg acgcatcacg cagggctgac cagaagtgac
     5641 agatccttat ctgaagattt atttgcagac gggttgttgc aagtattggt atgcactgcg
     5701 acgttagcat ggggtgttaa tctacctgct catacagtga ttataaaagg aacagatgtc
     5761 tattcacctg aaaaggggtc ctgggaacaa ctttctccac aagatgtact tcaaatgtta
     5821 ggtagagcag gcagaccgag atatgacaca tttggcgagg gtattataat aacagaccaa
     5881 tcgaatgttc aatattatct ctcggtttta aatcaacaat taccaataga atcgcaattt
     5941 gtttcaaaat tagttgacaa cttaaatgca gaagttgtcg cgggtaacat taagtgcagg
     6001 aatgatgctg tgaattggtt ggcgtatact tacctatatg tcagaatgtt agcttcccct
     6061 atgctttaca aagtacctga tatttcaagt gatgggcaat tgaaaaagtt tagagaaagc
     6121 ttagtccatt cggccctatg cattttaaaa gaacaagagt tggtgttata tgatgctgaa
     6181 aatgatgtaa ttgaggcgac cgatctagga aatatagcgt cgtcatttta cataaatcat
     6241 gcttccatgg atgtttacaa tagagagtta gatgaacata caactcagat tgatcttttt
     6301 agaatattct caatgtcgga agagtttaag tacgtatcgg tgagatatga agagaaaaga
     6361 gaactaaaac aacttctaga gaaagcaccc attcctatca gagaagatat tgatgatcct
     6421 ttagcaaaag tgaatgtact actgcagtct tatttttccc agctaaagtt cgaaggtttt
     6481 gcacttaatt ctgatatagt gtttattcac caaaatgctg gtaggttatt gcgtgctatg
     6541 tttgaaatat gcttgaagag aggatgggga catcccacac ggatgttgtt aaatctttgc
     6601 aaatctgcca caactaaaat gtggccaaca aactgtcctt taagacagtt taaaacatgc
     6661 cctgtcgaag tgattaaaag gctggaagcc tctacagtac cgtggggaga ttatctacag
     6721 ctagaaacac ctgccgaagt tggtcgtgca atcagatctg aaaaatatgg aaaacaggtt
     6781 tacgatttat tgaaaagatt tcccaaaatg tccgtaacat gcaacgctca gccaattacc
     6841 cgtagcgtta tgaggttcaa cattgaaata atagccgact ggatatggga tatgaatgta
     6901 cacggttccc ttgaaccttt tttgctcatg ctagaagaca ctgatggtga ttcaatactt
     6961 tattatgacg tactattcat cactccagat atagttggtc atgaatttac cttatccttt
     7021 acttatgagt taaagcagca caatcaaaat aacttaccac ctaatttctt ccttactttg
     7081 atatcagaaa attggtggca cagtgaattc gagattccag tttccttcaa tggatttaaa
     7141 cttccgaaaa aatttcctcc tcctactccc cttctggaga atattagtat ttcaacatca
     7201 gagcttggca atgatgattt ttccgaagta tttgaattta aaactttcaa caagattcag
     7261 agccaggtct tcgagtctct ctacaattcg aatgactcgg tctttgtagg ttcaggaaag
     7321 ggcacaggta aaacagctat ggctgaatta gcactgttaa atcattggag acagaataaa
     7381 ggaagagcgg tttatatcaa tccgtctggg gaaaaaattg attttttgct atctgattgg
     7441 aataaaaggt tctctcatct tgctggtggt aagataataa ataagctagg aaatgatcct
     7501 tcactaaatt taaaattatt agcaaaaagc catgttctct tggctacccc cgtgcaattt
     7561 gagcttttgt cacgtcgttg gagacaaagg aagaacatcc aaagtttaga attaatgata
     7621 tatgatgacg ctcatgagat tagccaaggt gtttacggtg ctgtgtatga aacgctaatt
     7681 tctagaatga tatttatagc aacccaactt gaaaagaaaa ttcgatttgt ttgcctttcg
     7741 aactgtttgg caaatgctcg tgattttggt gaatgggctg gtatgacgaa gtcaaacatc
     7801 tacaactttt ctccaagcga aagaattgag cctcttgaaa ttaatattca gtcatttaaa
     7861 gatgttgagc acatatcatt caatttttcg atgcttcaaa tggcatttga agcatctgct
     7921 gctgcagcgg gaaatcgtaa ttccagttcg gtgttcttac catctagaaa ggattgtatg
     7981 gaagttgcgt ctgcatttat gaagttttca aaagcaattg aatgggatat gttgaacgtt
     8041 gaagaagagc aaattgtacc atacattgaa aagttaacag atggtcatct aagagcacct
     8101 ttaaaacacg gtgttggtat tttgtataag gggatggcat caaatgatga aagaattgtg
     8161 aaaaggttat acgaatatgg tgcggtatcg gttttactca tttctaagga ttgttctgca
     8221 ttcgcttgta aaactgatga agtgattatt ttaggaacca acctttatga tggtgcagag
     8281 cataaatata tgccatatac cataaatgaa cttctagaaa tggtgggact agctagtggt
     8341 aacgattcaa tggcaggcaa ggttttaatt ttgacaagtc acaacatgaa ggcctactac
     8401 aaaaaattct taattgagcc tttgcctact gagagttacc tacagtatat tattcatgat
     8461 actctgaaca acgagatagc aaattctatt atacaaagca aacaagattg tgttgactgg
     8521 tttacatatt cgtatttcta tcgtcgtatc catgtgaatc caagctatta tggcgtaagg
     8581 gatacgtctc cacatggaat ttcagttttt ttgagtaatt tagttgaaac ttgtttgaat
     8641 gacttagttg aatcatcctt cattgaaatt gacgatacag aagctgaagt aaccgcagaa
     8701 gttaatggag gagatgatga agcaactgaa atcatttcta ccttgagcaa cgggctaatt
     8761 gcttctcact atggcgtatc attttttact attcagtcat tcgtttcttc tttatcgaat
     8821 acgtcaacgc tcaaaaacat gctgtatgtt ctttcaacag cagtagaatt cgaaagtgtt
     8881 ccactcagaa aaggagacag agcattactt gtcaaattaa gtaaaagact accattgaga
     8941 ttccctgaac acacctcttc tggttcggtg agctttaaag ttttcttatt gttacaagca
     9001 tatttttcac gtcttgaatt acctgtcgac tttcaaaatg atttaaaaga cattctagaa
     9061 aaggttgttc cactaattaa cgtagtggtt gatatccttt cagcaaacgg gtatttgaat
     9121 gctactaccg ccatggattt ggcgcagatg ttgattcagg gtgtatggga cgttgataat
     9181 ccgttgagac agattccaca cttcaacaac aaaattttgg aaaaatgtaa agaaattaac
     9241 gtcgagacgg tctatgatat aatggccctt gaggatgaag agagggatga aattttaacg
     9301 cttactgatt cacagcttgc gcaggtcgcc gcgtttgtta acaattaccc caacgttgaa
     9361 cttacttatt cgttgaataa ttctgattcg ttaatttcag gtgtaaaaca gaaaattacc
     9421 attcaattga caagagatgt tgaacctgag aacttacaag taacatctga aaagtatccg
     9481 tttgataagc tagaaagttg gtggttggtt ttaggtgaag tatctaaaaa agagctttat
     9541 gcgatcaaga aagttacttt aaataaagag actcagcaat atgaattgga atttgacact
     9601 ccgacatctg gtaaacataa cttaacaatt tggtgtgtct gtgattcata tcttgacgca
     9661 gataaagagt tgtcctttga aataaatgtg aaataaaatc attgtttagt cctggataat
     9721 cgaatggatt tcaatatata acattctctt gcggctattt aatctaattg tgatatatac
     9781 agtttatagc aaaagcgtat cattgcatca ctgcatttct atatcttcat cttcatcttc
     9841 atcctgttct ccttctttgt tttcgttttc aataaatcca ccttgatcct ctagagtcga
     9901 cctgcagggg tggagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag
     9961 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    10021 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    10081 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
    10141 tctccttacg catctgtgcg gtatttcaca ccgcataact agcttttaac ttgtatccta
    10201 ggttatctat gctgtctcac catagagaat attacctatt tcagaatgta tgtccatgat
    10261 tcgccgggta aatacatata atacacaaat ctggcttaat aaagtctata atatatctca
    10321 taaagaagtg ctaaattggc tagtgctata tatttttaag aaaatttctt ttgactaagt
    10381 ccatatcgac tttgtaaaag ttcacattag catacatata ttacacgagc cagaaatagt
    10441 aacttttgcc taaatcacaa attgcaaaat ttaattgctt gcaaaaggtc acatgcttat
    10501 aatcaacttt tttaaaaatt taaaatactt ttttattttt tatttttaaa cataaatgaa
    10561 ataatttatt tattgtttat gattaccgaa acataaaacc tgctcaagaa aaagaaactg
    10621 ttttgtcctt ggaaaaaaag cactacctag gagcggccaa aatgccgagg ctttcatagc
    10681 ttaaactctt tacagaaaat aggcattata gatcagttcg agttttctta ttcttccttc
    10741 cggttttatc gtcacagttt tacagtaaat aagtatcacc tcttagagtt agcttacatt
    10801 ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga ttaaatggcg
    10861 ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac tctaacaaaa
    10921 tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat ttatttaagt
    10981 attgtttgtg cacttgcctg caggcctttt gaaaagcaag cataaaagat ctaaacataa
    11041 aatctgtaaa ataacaagat gtaaagataa tgctaaatca tttggctttt tgattgattg
    11101 tacaggaaaa tatacatcgc agggggttga cttttaccat ttcaccgcaa tggaatcaaa
    11161 cttgttgaag agaatgttca caggcgcata cgctacaatg acccgattct tgctagcctt
    11221 ttctcggtct tgcaaacaac cgccggcagc ttagtatata aatacacatg tacatacctc
    11281 tctccgtatc ctcgtaatca ttttcttgta tttatcgtct tttcgctgta aaaactttat
    11341 cacacttatc tcaaatacac ttattaaccg cttttactat tatcttctac gctgacagta
    11401 atatcaaaca gtgacacata ttaaacacag tggtttcttt gcataaacac catcagcctc
    11461 aagtcgtcaa gtaaagattt cgtgttcatg cagatagata acaatctata tgttgataat
    11521 tagcgttgcc tcatcaatgc gagatccgtt taaccggacc ttttctttga ctacaacaca
    11581 aaaatcaagc acagaacgtc atacgaatcc gcaactacat tctgggcatt atgggctgga
    11641 cttgccacga aggagcaagc acagaaaatg gtggagaaag cactacccaa gttagagatg
    11701 cttggaggtt tagctgcatg tacggagcgt tctcgagttc aagagaaaaa aaaagaaaaa
    11761 gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa tgatgcatta
    11821 ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc ataggtatac
    11881 atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg tcactacata
    11941 agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc ttctcttatg
    12001 gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt gcctcgcaga
    12061 caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa atgcgggatc
    12121 atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac aactgcgtac
    12181 ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg cgcaaatcct
    12241 gatccaaacc tttttactcc acgcacggcc cctagggcct ctttaaaagc ttgaccgaga
    12301 gcaatcccgc agtcttcagt ggtgtgatgg tcgtctatgt gtaagtcacc aatgcactca
    12361 acgattagcg accagccgga atgcttggcc agagcatgta tcatatggtc cagaaaccct
    12421 atacctgtgt ggacgttaat cacttgcgat tgtgtggcct gttctgctac tgcttctgcc
    12481 tctttttctg ggaagatcga gtg
//
