LOCUS       sequence-254645-       11893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To make virus expressing Doc2beta Halotag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Courtney NA, Briguglio JS, Bradberry MM, Greer C, Chapman ER
  TITLE     Excitatory and Inhibitory Neurons Utilize Different Ca(2+) Sensors 
            and Sources to Regulate Spontaneous Release.
  JOURNAL   Neuron. 2018 Jun 6;98(5):977-991.e5. doi: 
            10.1016/j.neuron.2018.04.022. Epub 2018 May 10.
  PUBMED    29754754
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.04.022"; journalName: "Neuron"; date: 
            "2018-06-6- 6"; volume: "98"; issue: "5"; pages: "977-991.e5"
COMMENT     Sequence Label: pFSW Doc2beta Halotag
FEATURES             Location/Qualifiers
     source          1..11893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    493..726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             911..955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1253..1370
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1415..1861
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             3156..3176
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             3192..4073
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEIS"
     misc_feature    4082..4653
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4103..4655
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4247..4264)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4474..4493
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4655..4657,4658..4660,4661..5374)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4655..4657,4658..4660,4661..5371)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4700..4721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4961..4980)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5308..5329
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5398..5986
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5451..5471)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5869..5880)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5989..6005)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5990..6006)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6511..6691
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6717..6734)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6723..6947
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6993..7421
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7080..7099)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7290..7311
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7430..7450)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7435..7764
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7615..7750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7677..7696
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7812..7859
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7878..8252
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8382..8503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8419..8438)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8473..8492
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8552..8568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8552..8568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8565..8587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8576..8592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8600..8606,8607..8624,8625..8630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8645..8666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8783..8800)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8954..9542)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9034..9053)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9713..10504,10505..10573))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10336..10355
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10574..10678)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10753..10772)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10944..11323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11325..11523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11478..11498
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11541..11721
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11768..11893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      361 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
      421 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
      481 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
      541 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
      601 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
      661 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
      721 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
      781 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
      841 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
      901 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
      961 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1021 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1081 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1141 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1201 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     1261 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1321 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     1381 acagggacag cagagatcca gtttggttaa ttaaagtgca agtggtttta ggaccaggat
     1441 gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac
     1501 ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg
     1561 aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg
     1621 cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgta
     1681 aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca
     1741 ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga
     1801 ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca
     1861 ggtacggcta ggccaccatg accctccgaa gacgtggaga gaaggctaca atcagcatcc
     1921 aagagcatat ggcaatcgac gtgtgtcctg gacctatccg acctatcaag cagatctccg
     1981 attacttccc acgattccct cgtggactcc caccaacagc tgcaccacgt gcttcagcac
     2041 ctccagacgc tcctgcacga tcgccagcag ctaccgcagg tcctcgtagc ccctccgacg
     2101 gagcacgcga cgacgacgaa gatgtggacc agctcttcgg agcctacggt gccagcccag
     2161 gccccagccc cggacccagc cccgtgaggc cgccggccaa gccccccgag gacgaaccgg
     2221 acgccgacgg ctacgagtca gacgactgca ccgccctggg tacactggac ttcagtctgc
     2281 tctatgacca ggagaacaac gcactgcact gcaccatcag caaagccaag ggcctgaagc
     2341 cgatggatca caatggactg gctgatccct acgtcaaact acacttgctg cctggagcca
     2401 gcaaggcaaa taagctcaga acaaaaaccc tacggaacac tctgaacccc tcctggaacg
     2461 agaccctcac ctattacggg atcacggatg aggacatgat ccgaaagacc ctgaggatct
     2521 ccgtgtgtga cgaggacaaa ttccgccaca acgagttcat cggagagact cgagtgcccc
     2581 tgaagaagct gaaacccaac cacaccaaga cattcagcat ctgcctggag aagcagctgc
     2641 cggtggacaa ggcagaggac aagtccctgg aggagcgtgg ccgcatcctc atctctctca
     2701 agtacagctc acagaagcag ggcctgctgg tgggcatcgt tcgctgcgca cacctggcgg
     2761 ccatggatgc taacggctac tcagacccct acgtgaaaac atatctgaaa ccagatgtag
     2821 acaagaaatc caagcataag accgcagtca agaagaaaac actaaaccct gaattcaatg
     2881 aggagttctg ttacgagatc aagcatggag acctagccaa aaagaccctg gaggtcactg
     2941 tctgggacta tgatattgga aaatccaatg atttcatcgg tggggtggtt ctgggcatca
     3001 atgccaaggg tgagcgcctg aagcactggt ttgactgtct gaagaacaag gacaagagga
     3061 ttgagcgttg gcacacgctc accaatgaga tcccaggggc tgtactcagc gacggatccg
     3121 gaagttcggg gtccagcggt tcttcaggca gttccgagga tctgtacttt cagagcgata
     3181 acgatggatc cgaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg
     3241 gcgagcgcat gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc
     3301 acggtaaccc gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc
     3361 atcgctgcat tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt
     3421 atttcttcga cgaccacgtc cgcttcatgg atgccttcat cgaagccctg ggtctggaag
     3481 aggtcgtcct ggtcattcac gactggggct ccgctctggg tttccactgg gccaagcgca
     3541 atccagagcg cgtcaaaggt attgcattta tggagttcat ccgccctatc ccgacctggg
     3601 acgaatggcc agaatttgcc cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc
     3661 gcaagctgat catcgatcag aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc
     3721 gcccgctgac tgaagtcgag atggaccatt accgcgagcc gttcctgaat cctgttgacc
     3781 gcgagccact gtggcgcttc ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg
     3841 tcgcgctggt cgaagaatac atggactggc tgcaccagtc ccctgtcccg aagctgctgt
     3901 tctggggcac cccaggcgtt ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc
     3961 tgcctaactg caaggctgtg gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc
     4021 cggacctgat cggcagcgag atcgcgcgct ggctgtctac tctggagatt tcctgaaatt
     4081 ccccctctcc ctcccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4141 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4201 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4261 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4321 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4381 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     4441 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     4501 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac
     4561 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     4621 gacgtggttt tcctttgaaa aacacgatga taatatggtg agcaagggcg aggagctgtt
     4681 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4741 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4801 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4861 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4921 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4981 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5041 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     5101 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     5161 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5221 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     5281 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5341 gatcactctc ggcatggacg agctgtacaa gtaaaattcg atatcaagct tatcgataat
     5401 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5461 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5521 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5581 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5641 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5701 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5761 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     5821 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5881 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5941 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     6001 cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     6061 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     6121 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6181 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     6241 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     6301 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     6361 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     6421 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     6481 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     6541 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     6601 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     6661 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     6721 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6781 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6841 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6901 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6961 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     7021 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7081 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7141 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7201 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7261 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7321 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7381 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7441 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7501 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7561 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7621 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7681 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7741 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7801 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     7861 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     7921 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     7981 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     8041 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     8101 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     8161 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     8221 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     8281 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     8341 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     8401 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     8461 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     8521 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     8581 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8641 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8701 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8761 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8821 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8881 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8941 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     9001 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     9061 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     9121 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     9181 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     9241 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9301 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9361 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9421 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9481 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9541 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9601 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9661 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9721 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9781 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9841 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9901 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9961 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    10021 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    10081 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    10141 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    10201 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    10261 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10321 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10381 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10441 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10501 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10561 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10621 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10681 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    10741 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    10801 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    10861 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
    10921 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
    10981 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    11041 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    11101 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    11161 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    11221 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    11281 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
    11341 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
    11401 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
    11461 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
    11521 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
    11581 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
    11641 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
    11701 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
    11761 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
    11821 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
    11881 gtgcgagagc gtc
//
