LOCUS       sequence-25465-e         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Voutev R, Hubbard EJ
  TITLE     A ""FLP-Out"" system for controlled gene expression in 
            Caenorhabditis elegans.
  JOURNAL   Genetics. 2008 Sep;180(1):103-19. doi: 10.1534/genetics.108.090274.
  PUBMED    18723890
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.108"; journalName: "Genetics"; date: "2008-09"; 
            volume: "180"; issue: "1"; pages: "103-19"
COMMENT     Sequence Label: pGC75
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..42)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    81..128
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tttactcatt ttttctaccg gtactttgcg tttcttttta ggtgccgtca ttttttctga
       61 gctcggtacc cggggatctt gaagttccta ttccgaagtt cctattctct agaaagtata
      121 ggaacttcag agcgcttttg aagctagaac tagtggatcc cccgggctgc agcagattct
      181 tgatagccca gctccgcaaa atgtgagttt ggaagatttg gataattggt taattgaaaa
      241 aaaacacatg gaaattctat gaaattacct attgcaccat gttcttccaa cattttttaa
      301 aactaaaaac tagtgtcaga caacagaaaa tcgctcgatt tatctgaaaa tgacatttat
      361 ttcagctgca attttttttt gaaaatgctt aaaaatggtc tgaaaattgc caatttccga
      421 ttttctacat tttttcatta acttttaatg aaaaatgttg gagcaacatg gtgcaataag
      481 taaatttgta ggattgtcga gaataatttt tttaatcctc ctgaatattt tcaaaagttc
      541 gtattattcc caatttctag gtcaccctaa t
//
